Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A3DCH1

- CELK_CLOTH

UniProt

A3DCH1 - CELK_CLOTH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Cellulose 1,4-beta-cellobiosidase

Gene

celK

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei737 – 7371By similarity
Active sitei786 – 7861By similarity
Active sitei795 – 7951By similarity

GO - Molecular functioni

  1. cellulase activity Source: InterPro
  2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-429-MONOMER.
MetaCyc:MONOMER-16421.

Protein family/group databases

CAZyiCBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Cellulose 1,4-beta-cellobiosidase (EC:3.2.1.91)
Gene namesi
Name:celK
Ordered Locus Names:Cthe_0412
ORF Names:CtheDRAFT_2165
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 895868Cellulose 1,4-beta-cellobiosidasePRO_0000284718Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0412.

Structurei

3D structure databases

ProteinModelPortaliA3DCH1.
SMRiA3DCH1. Positions 208-813, 831-895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 199160CBM-cenCAdd
BLAST
Domaini834 – 85421Dockerin 1Add
BLAST
Domaini866 – 88621Dockerin 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 24042LinkerBy similarityAdd
BLAST
Regioni241 – 815575CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Contains 2 dockerin domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG326123.
HOGENOMiHOG000245359.
OMAiQAGVCDY.
OrthoDBiEOG6QRW4D.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DCH1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSPKLPDYKN DLLYERTFDE
60 70 80 90 100
GLCFPWHTCE DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH
110 120 130 140 150
RGITLEQGHT YTVRFTIWSD KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT
160 170 180 190 200
PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE LAAGTPYYVY LDDVSLYDPR
210 220 230 240 250
FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK WQLLNSANQV
260 270 280 290 300
VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP
310 320 330 340 350
FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGVAPN
360 370 380 390 400
KGDTNVPTWP QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT
410 420 430 440 450
LMNMYERAKI RGIANQGAYK DGGMNIPERN NGYPDILDEA RWEIEFFKKM
460 470 480 490 500
QVTEKEDPSI AGMVHHKIHD FRWTALGMLP HEDPQPRYLR PVSTAATLNF
510 520 530 540 550
AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY AEYTPGSGGP
560 570 580 590 600
GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN
610 620 630 640 650
GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPSADIQ KARNNIAKAA
660 670 680 690 700
DKWLENIEEQ GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN
710 720 730 740 750
SKYLDGMQDG MSYLLGRNGL DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP
760 770 780 790 800
APPPGIIAGG PNSRFEDPTI TAAVKKDTPP QKCYIDHTDS WSTNEITINW
810 820 830 840 850
NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK VNSTDAVALK
860 870 880 890
RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK
Length:895
Mass (Da):100,622
Last modified:March 20, 2007 - v1
Checksum:i9354DFE9EC75BE67
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN51650.1.
RefSeqiYP_001036843.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51650; ABN51650; Cthe_0412.
GeneIDi4808415.
KEGGicth:Cthe_0412.
PATRICi19514485. VBICloThe47081_0437.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN51650.1 .
RefSeqi YP_001036843.1. NC_009012.1.

3D structure databases

ProteinModelPortali A3DCH1.
SMRi A3DCH1. Positions 208-813, 831-895.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_0412.

Protein family/group databases

CAZyi CBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN51650 ; ABN51650 ; Cthe_0412 .
GeneIDi 4808415.
KEGGi cth:Cthe_0412.
PATRICi 19514485. VBICloThe47081_0437.

Phylogenomic databases

eggNOGi NOG326123.
HOGENOMi HOG000245359.
OMAi QAGVCDY.
OrthoDBi EOG6QRW4D.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-429-MONOMER.
MetaCyc:MONOMER-16421.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
Pfami PF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiCELK_CLOTH
AccessioniPrimary (citable) accession number: A3DCH1
Secondary accession number(s): O68438, Q10748, Q4CGG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3