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A3DCH1 (CELK_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cellulose 1,4-beta-cellobiosidase

EC=3.2.1.91
Gene names
Name:celK
Ordered Locus Names:Cthe_0412
ORF Names:CtheDRAFT_2165
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length895 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Contains 1 CBM-cenC (cenC-type cellulose-binding) domain.

Contains 2 dockerin domains.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: InterPro

cellulose 1,4-beta-cellobiosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 By similarity
Chain28 – 895868Cellulose 1,4-beta-cellobiosidase
PRO_0000284718

Regions

Domain40 – 199160CBM-cenC
Domain834 – 85421Dockerin 1
Domain866 – 88621Dockerin 2
Region199 – 24042Linker By similarity
Region241 – 815575Catalytic By similarity

Sites

Active site7371 By similarity
Active site7861 By similarity
Active site7951 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3DCH1 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 9354DFE9EC75BE67

FASTA895100,622
        10         20         30         40         50         60 
MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSPKLPDYKN DLLYERTFDE GLCFPWHTCE 

        70         80         90        100        110        120 
DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH RGITLEQGHT YTVRFTIWSD 

       130        140        150        160        170        180 
KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE 

       190        200        210        220        230        240 
LAAGTPYYVY LDDVSLYDPR FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK 

       250        260        270        280        290        300 
WQLLNSANQV VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP 

       310        320        330        340        350        360 
FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGVAPN KGDTNVPTWP 

       370        380        390        400        410        420 
QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT LMNMYERAKI RGIANQGAYK 

       430        440        450        460        470        480 
DGGMNIPERN NGYPDILDEA RWEIEFFKKM QVTEKEDPSI AGMVHHKIHD FRWTALGMLP 

       490        500        510        520        530        540 
HEDPQPRYLR PVSTAATLNF AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY 

       550        560        570        580        590        600 
AEYTPGSGGP GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN 

       610        620        630        640        650        660 
GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPSADIQ KARNNIAKAA DKWLENIEEQ 

       670        680        690        700        710        720 
GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN SKYLDGMQDG MSYLLGRNGL 

       730        740        750        760        770        780 
DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP APPPGIIAGG PNSRFEDPTI TAAVKKDTPP 

       790        800        810        820        830        840 
QKCYIDHTDS WSTNEITINW NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK 

       850        860        870        880        890 
VNSTDAVALK RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK 

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References

[1]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000568 Genomic DNA. Translation: ABN51650.1.
RefSeqYP_001036843.1. NC_009012.1.

3D structure databases

ProteinModelPortalA3DCH1.
SMRA3DCH1. Positions 208-813, 831-895.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_0412.

Protein family/group databases

CAZyCBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN51650; ABN51650; Cthe_0412.
GeneID4808415.
KEGGcth:Cthe_0412.
PATRIC19514485. VBICloThe47081_0437.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG326123.
HOGENOMHOG000245359.
OMAYYTQRSG.
OrthoDBEOG6QRW4D.
ProtClustDBCLSK734822.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-429-MONOMER.
MetaCyc:MONOMER-16421.

Family and domain databases

Gene3D1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR003305. CenC_carb-bd.
IPR018242. Dockerin_1.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR004197. Glyco_hydro_9_Ig-like.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF02018. CBM_4_9. 1 hit.
PF02927. CelD_N. 1 hit.
PF00404. Dockerin_1. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF63446. SSF63446. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCELK_CLOTH
AccessionPrimary (citable) accession number: A3DCH1
Secondary accession number(s): O68438, Q10748, Q4CGG7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: November 13, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries