Reviewed, UniProtKB/Swiss-Prot A3DCH1 (CELK_CLOTH)

Names and origin

Protein names

Recommended name:
    Cellulose 1,4-beta-cellobiosidase
    EC=3.2.1.91

Gene names

Name:	celK
Ordered Locus Names:	Cthe_0412
ORF Names:	CtheDRAFT_2165

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	895 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM-cenC (cenC-type cellulose-binding) domain. Contains 2 dockerin domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: InterPro cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Chain	28 – 895	868	Cellulose 1,4-beta-cellobiosidase		PRO_0000284718
Regions
	Domain	40 – 199	160	CBM-cenC
	Domain	834 – 854	21	Dockerin 1
	Domain	866 – 886	21	Dockerin 2
	Region	199 – 240	42	Linker By similarity
	Region	241 – 815	575	Catalytic By similarity
Sites
	Active site	737	1	By similarity
	Active site	786	1	By similarity
	Active site	795	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3DCH1-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 9354DFE9EC75BE67	FASTA	895	100,622
	10 20 30 40 50 60 MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSPKLPDYKN DLLYERTFDE GLCFPWHTCE 70 80 90 100 110 120 DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH RGITLEQGHT YTVRFTIWSD 130 140 150 160 170 180 KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE 190 200 210 220 230 240 LAAGTPYYVY LDDVSLYDPR FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK 250 260 270 280 290 300 WQLLNSANQV VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP 310 320 330 340 350 360 FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGVAPN KGDTNVPTWP 370 380 390 400 410 420 QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT LMNMYERAKI RGIANQGAYK 430 440 450 460 470 480 DGGMNIPERN NGYPDILDEA RWEIEFFKKM QVTEKEDPSI AGMVHHKIHD FRWTALGMLP 490 500 510 520 530 540 HEDPQPRYLR PVSTAATLNF AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY 550 560 570 580 590 600 AEYTPGSGGP GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN 610 620 630 640 650 660 GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPSADIQ KARNNIAKAA DKWLENIEEQ 670 680 690 700 710 720 GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN SKYLDGMQDG MSYLLGRNGL 730 740 750 760 770 780 DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP APPPGIIAGG PNSRFEDPTI TAAVKKDTPP 790 800 810 820 830 840 QKCYIDHTDS WSTNEITINW NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK 850 860 870 880 890 VNSTDAVALK RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK

References

[1]

"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000568 Genomic DNA. Translation: ABN51650.1.
RefSeq	YP_001036843.1.
3D structure databases
SMR	A3DCH1. Positions 831-895.
ModBase	Search...
Protein-protein interaction databases
STRING	A3DCH1.
Genome annotation databases
GeneID	4808415.
GenomeReviews	Gene locus Cthe_0412 in contig CP000568_GR.
KEGG	cth:Cthe_0412.
NMPDR	fig|203119.1.peg.2880.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LAHDFTG
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR003305. CenC_carb_bd. IPR018242. Dockerin_1. IPR008979. Galactose-bd-like. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. IPR004197. Glyco_hydro_9_Ig-like. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF02018. CBM_4_9. 1 hit. PF02927. CelD_N. 1 hit. PF00404. Dockerin_1. 2 hits. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00448. CLOS_CELLULOSOME_RPT. 1 hit. PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CELK_CLOTH

Accession

Primary (citable) accession number: A3DCH1
Secondary accession number(s): O68438, Q10748, Q4CGG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	March 20, 2007
Last modified:	November 24, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)