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A3DCH1

- CELK_CLOTH

UniProt

A3DCH1 - CELK_CLOTH

Protein

Cellulose 1,4-beta-cellobiosidase

Gene

celK

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei737 – 7371By similarity
    Active sitei786 – 7861By similarity
    Active sitei795 – 7951By similarity

    GO - Molecular functioni

    1. cellulase activity Source: InterPro
    2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-429-MONOMER.
    MetaCyc:MONOMER-16421.

    Protein family/group databases

    CAZyiCBM4. Carbohydrate-Binding Module Family 4.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cellulose 1,4-beta-cellobiosidase (EC:3.2.1.91)
    Gene namesi
    Name:celK
    Ordered Locus Names:Cthe_0412
    ORF Names:CtheDRAFT_2165
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727By similarityAdd
    BLAST
    Chaini28 – 895868Cellulose 1,4-beta-cellobiosidasePRO_0000284718Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi203119.Cthe_0412.

    Structurei

    3D structure databases

    ProteinModelPortaliA3DCH1.
    SMRiA3DCH1. Positions 208-813, 831-895.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 199160CBM-cenCAdd
    BLAST
    Domaini834 – 85421Dockerin 1Add
    BLAST
    Domaini866 – 88621Dockerin 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni199 – 24042LinkerBy similarityAdd
    BLAST
    Regioni241 – 815575CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 2 dockerin domains.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG326123.
    HOGENOMiHOG000245359.
    OMAiQAGVCDY.
    OrthoDBiEOG6QRW4D.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    2.60.120.260. 1 hit.
    2.60.40.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR003305. CenC_carb-bd.
    IPR018242. Dockerin_1.
    IPR008979. Galactose-bd-like.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR004197. Glyco_hydro_9_Ig-like.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF02018. CBM_4_9. 1 hit.
    PF02927. CelD_N. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF63446. SSF63446. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A3DCH1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNFRRMLCAA IVLTIVLSIM LPSTVFALED KSPKLPDYKN DLLYERTFDE    50
    GLCFPWHTCE DSGGKCDFAV VDVPGEPGNK AFRLTVIDKG QNKWSVQMRH 100
    RGITLEQGHT YTVRFTIWSD KSCRVYAKIG QMGEPYTEYW NNNWNPFNLT 150
    PGQKLTVEQN FTMNYPTDDT CEFTFHLGGE LAAGTPYYVY LDDVSLYDPR 200
    FVKPVEYVLP QPDVRVNQVG YLPFAKKYAT VVSSSTSPLK WQLLNSANQV 250
    VLEGNTIPKG LDKDSQDYVH WIDFSNFKTE GKGYYFKLPT VNSDTNYSHP 300
    FDISADIYSK MKFDALAFFY HKRSGIPIEM PYAGGEQWTR PAGHIGVAPN 350
    KGDTNVPTWP QDDEYAGRPQ KYYTKDVTGG WYDAGDHGKY VVNGGIAVWT 400
    LMNMYERAKI RGIANQGAYK DGGMNIPERN NGYPDILDEA RWEIEFFKKM 450
    QVTEKEDPSI AGMVHHKIHD FRWTALGMLP HEDPQPRYLR PVSTAATLNF 500
    AATLAQSARL WKDYDPTFAA DCLEKAEIAW QAALKHPDIY AEYTPGSGGP 550
    GGGPYNDDYV GDEFYWAACE LYVTTGKDEY KNYLMNSPHY LEMPAKMGEN 600
    GGANGEDNGL WGCFTWGTTQ GLGTITLALV ENGLPSADIQ KARNNIAKAA 650
    DKWLENIEEQ GYRLPIKQAE DERGGYPWGS NSFILNQMIV MGYAYDFTGN 700
    SKYLDGMQDG MSYLLGRNGL DQSYVTGYGE RPLQNPHDRF WTPQTSKKFP 750
    APPPGIIAGG PNSRFEDPTI TAAVKKDTPP QKCYIDHTDS WSTNEITINW 800
    NAPFAWVTAY LDEIDLITPP GGVDPEEPEV IYGDCNGDGK VNSTDAVALK 850
    RYILRSGISI NTDNADVNAD GRVNSTDLAI LKRYILKEID VLPHK 895
    Length:895
    Mass (Da):100,622
    Last modified:March 20, 2007 - v1
    Checksum:i9354DFE9EC75BE67
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN51650.1.
    RefSeqiYP_001036843.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN51650; ABN51650; Cthe_0412.
    GeneIDi4808415.
    KEGGicth:Cthe_0412.
    PATRICi19514485. VBICloThe47081_0437.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN51650.1 .
    RefSeqi YP_001036843.1. NC_009012.1.

    3D structure databases

    ProteinModelPortali A3DCH1.
    SMRi A3DCH1. Positions 208-813, 831-895.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203119.Cthe_0412.

    Protein family/group databases

    CAZyi CBM4. Carbohydrate-Binding Module Family 4.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN51650 ; ABN51650 ; Cthe_0412 .
    GeneIDi 4808415.
    KEGGi cth:Cthe_0412.
    PATRICi 19514485. VBICloThe47081_0437.

    Phylogenomic databases

    eggNOGi NOG326123.
    HOGENOMi HOG000245359.
    OMAi QAGVCDY.
    OrthoDBi EOG6QRW4D.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-429-MONOMER.
    MetaCyc:MONOMER-16421.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    1.50.10.10. 1 hit.
    2.60.120.260. 1 hit.
    2.60.40.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR003305. CenC_carb-bd.
    IPR018242. Dockerin_1.
    IPR008979. Galactose-bd-like.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    IPR004197. Glyco_hydro_9_Ig-like.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF02018. CBM_4_9. 1 hit.
    PF02927. CelD_N. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF63446. SSF63446. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiCELK_CLOTH
    AccessioniPrimary (citable) accession number: A3DCH1
    Secondary accession number(s): O68438, Q10748, Q4CGG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3