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A3DC45

- A3DC45_CLOTH

UniProt

A3DC45 - A3DC45_CLOTH

Protein

Isocitrate dehydrogenase [NADP]

Gene

Cthe_0285

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771SubstrateUniRule annotation
    Binding sitei82 – 821NADPImported
    Binding sitei96 – 961NADPImported
    Binding sitei109 – 1091SubstrateUniRule annotation
    Binding sitei132 – 1321SubstrateUniRule annotation
    Sitei139 – 1391Critical for catalysisUniRule annotation
    Sitei210 – 2101Critical for catalysisUniRule annotation
    Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation
    Binding sitei255 – 2551NADPImported
    Metal bindingi273 – 2731Magnesium or manganeseUniRule annotation
    Binding sitei325 – 3251NADP; via amide nitrogen and carbonyl oxygenImported
    Binding sitei372 – 3721NADPImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 773NADPImported
    Nucleotide bindingi306 – 3127NADPImported

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotationImported, Nucleotide-bindingImported

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-293-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    Ordered Locus Names:Cthe_0285Imported
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)Imported
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi203119.Cthe_0285.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AOUX-ray2.50A1-402[»]
    ProteinModelPortaliA3DC45.
    SMRiA3DC45. Positions 3-401.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni94 – 1007Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0538.
    HOGENOMiHOG000019858.
    KOiK00031.
    OMAiCVNTVET.
    OrthoDBiEOG628F3X.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A3DC45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIKMKVPL VEMDGDEMTR IIWRLIKENL LEPYIELNTE YYDLGLENRD    50
    KTEDQVTIDA ARAIQKYGVG VKCATITPNA QRVEEYNLKK MWKSPNGTIR 100
    AILDGTVFRA PIVVNSIKPF VKGWKKPISI ARHAYGDVYK NVEYYVPSAG 150
    KAELVFTSEN GEVSRQTIHE FDGPGVIMGM HNTDKSIRSF ARACFNYALD 200
    MNQDLWFSTK DTISKTYDHR FKDIFQEIYE NEYKEKFEAK NLQYFYTLID 250
    DAVARIIRSE GGMVWACKNY DGDVMSDMVA SAFGSLAMMT SVLVSPDGKY 300
    EFEAAHGTVT RHYYKHLKGE ETSTNSMATI FAWTGALKKR GELDGIKELV 350
    DFATKLEQAS VQTIENGVMT KDLASLSEVP EKKIVNTEDF LKEIRKTFEG 400
    MA 402
    Length:402
    Mass (Da):45,782
    Last modified:March 20, 2007 - v1
    Checksum:i5A55781A7AC5B42E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN51524.1.
    RefSeqiYP_001036717.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN51524; ABN51524; Cthe_0285.
    GeneIDi4808568.
    KEGGicth:Cthe_0285.
    PATRICi19514209. VBICloThe47081_0302.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000568 Genomic DNA. Translation: ABN51524.1 .
    RefSeqi YP_001036717.1. NC_009012.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AOU X-ray 2.50 A 1-402 [» ]
    ProteinModelPortali A3DC45.
    SMRi A3DC45. Positions 3-401.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203119.Cthe_0285.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN51524 ; ABN51524 ; Cthe_0285 .
    GeneIDi 4808568.
    KEGGi cth:Cthe_0285.
    PATRICi 19514209. VBICloThe47081_0302.

    Phylogenomic databases

    eggNOGi COG0538.
    HOGENOMi HOG000019858.
    KOi K00031.
    OMAi CVNTVET.
    OrthoDBi EOG628F3X.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-293-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372Imported.
    2. "The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila suggest a new active site locking mechanism."
      Leiros H.K., Fedoy A.E., Leiros I., Steen I.H.
      FEBS Open Bio 2:159-172(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH NADP.

    Entry informationi

    Entry nameiA3DC45_CLOTH
    AccessioniPrimary (citable) accession number: A3DC45
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 20, 2007
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3