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A3DC45

- A3DC45_CLOTH

UniProt

A3DC45 - A3DC45_CLOTH

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Protein
Isocitrate dehydrogenase [NADP]
Gene
Cthe_0285
Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237)
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771Substrate By similarityUniRule annotation
Binding sitei82 – 821NADPImported
Binding sitei96 – 961NADPImported
Binding sitei109 – 1091Substrate By similarityUniRule annotation
Binding sitei132 – 1321Substrate By similarityUniRule annotation
Sitei139 – 1391Critical for catalysis By similarity
Sitei210 – 2101Critical for catalysis By similarityUniRule annotation
Metal bindingi250 – 2501Magnesium or manganese By similarityUniRule annotation
Binding sitei255 – 2551NADPImported
Metal bindingi273 – 2731Magnesium or manganese By similarityUniRule annotation
Binding sitei325 – 3251NADP; via amide nitrogen and carbonyl oxygenImported
Binding sitei372 – 3721NADPImported

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPImported
Nucleotide bindingi306 – 3127NADPImported

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro

GO - Biological processi

  1. isocitrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotationImported, Nucleotide-bindingImported

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-293-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:Cthe_0285Imported
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237)Imported
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0285.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AOUX-ray2.50A1-402[»]
ProteinModelPortaliA3DC45.
SMRiA3DC45. Positions 3-401.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0538.
HOGENOMiHOG000019858.
KOiK00031.
OMAiCVNTVET.
OrthoDBiEOG628F3X.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3DC45-1 [UniParc]FASTAAdd to Basket

« Hide

MSKIKMKVPL VEMDGDEMTR IIWRLIKENL LEPYIELNTE YYDLGLENRD    50
KTEDQVTIDA ARAIQKYGVG VKCATITPNA QRVEEYNLKK MWKSPNGTIR 100
AILDGTVFRA PIVVNSIKPF VKGWKKPISI ARHAYGDVYK NVEYYVPSAG 150
KAELVFTSEN GEVSRQTIHE FDGPGVIMGM HNTDKSIRSF ARACFNYALD 200
MNQDLWFSTK DTISKTYDHR FKDIFQEIYE NEYKEKFEAK NLQYFYTLID 250
DAVARIIRSE GGMVWACKNY DGDVMSDMVA SAFGSLAMMT SVLVSPDGKY 300
EFEAAHGTVT RHYYKHLKGE ETSTNSMATI FAWTGALKKR GELDGIKELV 350
DFATKLEQAS VQTIENGVMT KDLASLSEVP EKKIVNTEDF LKEIRKTFEG 400
MA 402
Length:402
Mass (Da):45,782
Last modified:March 20, 2007 - v1
Checksum:i5A55781A7AC5B42E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN51524.1.
RefSeqiYP_001036717.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51524; ABN51524; Cthe_0285.
GeneIDi4808568.
KEGGicth:Cthe_0285.
PATRICi19514209. VBICloThe47081_0302.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000568 Genomic DNA. Translation: ABN51524.1 .
RefSeqi YP_001036717.1. NC_009012.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AOU X-ray 2.50 A 1-402 [» ]
ProteinModelPortali A3DC45.
SMRi A3DC45. Positions 3-401.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_0285.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN51524 ; ABN51524 ; Cthe_0285 .
GeneIDi 4808568.
KEGGi cth:Cthe_0285.
PATRICi 19514209. VBICloThe47081_0302.

Phylogenomic databases

eggNOGi COG0538.
HOGENOMi HOG000019858.
KOi K00031.
OMAi CVNTVET.
OrthoDBi EOG628F3X.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-293-MONOMER.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237.
  2. "The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila suggest a new active site locking mechanism."
    Leiros H.K., Fedoy A.E., Leiros I., Steen I.H.
    FEBS Open Bio 2:159-172(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) IN COMPLEX WITH NADP.

Entry informationi

Entry nameiA3DC45_CLOTH
AccessioniPrimary (citable) accession number: A3DC45
Entry historyi
Integrated into UniProtKB/TrEMBL: March 20, 2007
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome

External Data

Dasty 3

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