Isocitrate dehydrogenase [NADP] - Clostridium thermocellum (strain ATCC 27405 / DSM 1237)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Ordered Locus Names:

Cthe_0285

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP] EMBL ABN51524.1

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 PIRSR PIRSR000108-3 Manganese PIRNR PIRNR000108 PIRSR PIRSR000108-3 Metal-binding PIRNR PIRNR000108 PIRSR PIRSR000108-3 NADP PIRNR PIRNR000108
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444
Technical term	3D-structure PDB 4AOU Complete proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

94 – 100

7

Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding

250

1

Magnesium or manganese By similarity PIRSR PIRSR000108-3

Metal binding

273

1

Magnesium or manganese By similarity PIRSR PIRSR000108-3

Binding site

77

1

Substrate By similarity PIRSR PIRSR000108-2

Binding site

109

1

Substrate By similarity PIRSR PIRSR000108-2

Binding site

132

1

Substrate By similarity PIRSR PIRSR000108-2

Site

139

1

Critical for catalysis By similarity PIRSR PIRSR000108-1

Site

210

1

Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence

Length

Mass (Da)

Tools

A3DC45 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 5A55781A7AC5B42E
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FASTA

402

45,782

        10         20         30         40         50         60 
MSKIKMKVPL VEMDGDEMTR IIWRLIKENL LEPYIELNTE YYDLGLENRD KTEDQVTIDA 

        70         80         90        100        110        120 
ARAIQKYGVG VKCATITPNA QRVEEYNLKK MWKSPNGTIR AILDGTVFRA PIVVNSIKPF 

       130        140        150        160        170        180 
VKGWKKPISI ARHAYGDVYK NVEYYVPSAG KAELVFTSEN GEVSRQTIHE FDGPGVIMGM 

       190        200        210        220        230        240 
HNTDKSIRSF ARACFNYALD MNQDLWFSTK DTISKTYDHR FKDIFQEIYE NEYKEKFEAK 

       250        260        270        280        290        300 
NLQYFYTLID DAVARIIRSE GGMVWACKNY DGDVMSDMVA SAFGSLAMMT SVLVSPDGKY 

       310        320        330        340        350        360 
EFEAAHGTVT RHYYKHLKGE ETSTNSMATI FAWTGALKKR GELDGIKELV DFATKLEQAS 

       370        380        390        400 
VQTIENGVMT KDLASLSEVP EKKIVNTEDF LKEIRKTFEG MA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of Clostridium thermocellum ATCC 27405." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.
[2]	"The Complex Structures of Isocitrate Dehydrogenase from Clostridium Thermocellum and Desulfotalea Psychrophila, Support a New Active Site Locking Mechanism." Leiros H.-K.S., Fedoy A.-E., Leiros I., Steen I.H. Submitted (MAR-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000568 Genomic DNA. Translation: ABN51524.1.

RefSeq

YP_001036717.1. NC_009012.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AOU	X-ray	2.50	A	1-402	[»]

ProteinModelPortal

A3DC45.

SMR

A3DC45. Positions 3-401.

ModBase

Search...

Protein-protein interaction databases

STRING

203119.Cthe_0285.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ABN51524; ABN51524; Cthe_0285.

GeneID

4808568.

KEGG

cth:Cthe_0285.

PATRIC

19514209. VBICloThe47081_0302.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0538.

HOGENOM

HOG000019858.

KO

K00031.

OMA

QHQQGKP.

ProtClustDB

PRK08299.

Enzyme and pathway databases

BioCyc

CTHE203119:GIW8-293-MONOMER.

Family and domain databases

Gene3D

3.40.718.10. 1 hit.

InterPro

IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]

PANTHER

PTHR11822. PTHR11822. 1 hit.

Pfam

PF00180. Iso_dh. 1 hit.
[Graphical view]

PIRSF

PIRSF000108. IDH_NADP. 1 hit.

TIGRFAMs

TIGR00127. nadp_idh_euk. 1 hit.

PROSITE

PS00470. IDH_IMDH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

A3DC45_CLOTH

Accession

Primary (citable) accession number: A3DC45

Entry history

Integrated into UniProtKB/TrEMBL:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)