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Protein

Isocitrate dehydrogenase [NADP]

Gene

Cthe_0285

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Binding sitei82NADPCombined sources1
Binding sitei96NADPCombined sources1
Binding sitei109SubstrateUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Sitei139Critical for catalysisUniRule annotation1
Sitei210Critical for catalysisUniRule annotation1
Metal bindingi250MagnesiumCombined sources1
Metal bindingi250Magnesium or manganeseUniRule annotation1
Binding sitei255NADPCombined sources1
Metal bindingi273MagnesiumCombined sources1
Metal bindingi273Magnesium or manganeseUniRule annotation1
Binding sitei325NADP; via amide nitrogen and carbonyl oxygenCombined sources1
Binding sitei372NADPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPCombined sources3
Nucleotide bindingi306 – 312NADPCombined sources7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processTricarboxylic acid cycleUniRule annotation
LigandMagnesiumUniRule annotationCombined sources, ManganeseUniRule annotation, Metal-bindingUniRule annotationCombined sources, NADPUniRule annotationCombined sources, Nucleotide-bindingCombined sources

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:Cthe_0285Imported
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)Imported
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0285.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AOUX-ray2.50A1-402[»]
ProteinModelPortaliA3DC45.
SMRiA3DC45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 394Iso_dhInterPro annotationAdd BLAST388

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D5N. Bacteria.
COG0538. LUCA.
HOGENOMiHOG000019858.
KOiK00031.
OMAiAMGMYNQ.
OrthoDBiPOG091H0JP0.

Family and domain databases

InterProiView protein in InterPro
IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiView protein in Pfam
PF00180. Iso_dh. 1 hit.
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiView protein in SMART
SM01329. Iso_dh. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiView protein in PROSITE
PS00470. IDH_IMDH. 1 hit.

Sequencei

Sequence statusi: Complete.

A3DC45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKMKVPL VEMDGDEMTR IIWRLIKENL LEPYIELNTE YYDLGLENRD
60 70 80 90 100
KTEDQVTIDA ARAIQKYGVG VKCATITPNA QRVEEYNLKK MWKSPNGTIR
110 120 130 140 150
AILDGTVFRA PIVVNSIKPF VKGWKKPISI ARHAYGDVYK NVEYYVPSAG
160 170 180 190 200
KAELVFTSEN GEVSRQTIHE FDGPGVIMGM HNTDKSIRSF ARACFNYALD
210 220 230 240 250
MNQDLWFSTK DTISKTYDHR FKDIFQEIYE NEYKEKFEAK NLQYFYTLID
260 270 280 290 300
DAVARIIRSE GGMVWACKNY DGDVMSDMVA SAFGSLAMMT SVLVSPDGKY
310 320 330 340 350
EFEAAHGTVT RHYYKHLKGE ETSTNSMATI FAWTGALKKR GELDGIKELV
360 370 380 390 400
DFATKLEQAS VQTIENGVMT KDLASLSEVP EKKIVNTEDF LKEIRKTFEG

MA
Length:402
Mass (Da):45,782
Last modified:March 20, 2007 - v1
Checksum:i5A55781A7AC5B42E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA. Translation: ABN51524.1.
RefSeqiWP_003512448.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51524; ABN51524; Cthe_0285.
KEGGicth:Cthe_0285.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA3DC45_CLOTH
AccessioniPrimary (citable) accession number: A3DC45
Entry historyiIntegrated into UniProtKB/TrEMBL: March 20, 2007
Last sequence update: March 20, 2007
Last modified: July 5, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported