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Protein

Endoglucanase A

Gene

celA

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Proton donor1
Active sitei152NucleophileCurated1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-281-MONOMER
MetaCyc:MONOMER-16413

Protein family/group databases

CAZyiGH8 Glycoside Hydrolase Family 8

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Short name:
EGA
Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase
Gene namesi
Name:celA
Ordered Locus Names:Cthe_0269
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32By similarityAdd BLAST32
ChainiPRO_000028472133 – 477Endoglucanase AAdd BLAST445

Proteomic databases

PRIDEiA3DC29

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0269

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 44Combined sources3
Beta strandi48 – 50Combined sources3
Helixi52 – 70Combined sources19
Helixi86 – 88Combined sources3
Helixi94 – 106Combined sources13
Helixi110 – 121Combined sources12
Beta strandi128 – 130Combined sources3
Beta strandi132 – 134Combined sources3
Turni142 – 147Combined sources6
Helixi151 – 168Combined sources18
Beta strandi170 – 174Combined sources5
Helixi176 – 191Combined sources16
Turni194 – 196Combined sources3
Beta strandi201 – 205Combined sources5
Helixi213 – 215Combined sources3
Helixi218 – 228Combined sources11
Helixi232 – 247Combined sources16
Turni248 – 250Combined sources3
Beta strandi259 – 261Combined sources3
Beta strandi274 – 276Combined sources3
Helixi279 – 281Combined sources3
Helixi282 – 293Combined sources12
Helixi296 – 310Combined sources15
Helixi314 – 316Combined sources3
Turni334 – 336Combined sources3
Helixi337 – 344Combined sources8
Helixi350 – 362Combined sources13
Helixi367 – 369Combined sources3
Helixi372 – 384Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CEMX-ray1.65A33-395[»]
1IS9X-ray1.03A33-395[»]
1KWFX-ray0.94A33-395[»]
ProteinModelPortaliA3DC29
SMRiA3DC29
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA3DC29

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini411 – 477DockerinPROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F5Q Bacteria
ENOG410Y1JM LUCA
HOGENOMiHOG000069581
OMAiEAHGYGM
OrthoDBiPOG091H07QZ

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR018247 EF_Hand_1_Ca_BS
IPR002037 Glyco_hydro_8
IPR019834 Glyco_hydro_8_CS
PfamiView protein in Pfam
PF00404 Dockerin_1, 2 hits
PF01270 Glyco_hydro_8, 1 hit
PRINTSiPR00735 GLHYDRLASE8
SUPFAMiSSF48208 SSF48208, 1 hit
SSF63446 SSF63446, 1 hit
PROSITEiView protein in PROSITE
PS00448 CLOS_CELLULOSOME_RPT, 2 hits
PS51766 DOCKERIN, 1 hit
PS00018 EF_HAND_1, 2 hits
PS00812 GLYCOSYL_HYDROL_F8, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DC29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVKKRVGV VLLILAVLGV YMLAMPANTV SAAGVPFNTK YPYGPTSIAD
60 70 80 90 100
NQSEVTAMLK AEWEDWKSKR ITSNGAGGYK RVQRDASTNY DTVSEGMGYG
110 120 130 140 150
LLLAVCFNEQ ALFDDLYRYV KSHFNGNGLM HWHIDANNNV TSHDGGDGAA
160 170 180 190 200
TDADEDIALA LIFADKLWGS SGAINYGQEA RTLINNLYNH CVEHGSYVLK
210 220 230 240 250
PGDRWGGSSV TNPSYFAPAW YKVYAQYTGD TRWNQVADKC YQIVEEVKKY
260 270 280 290 300
NNGTGLVPDW CTASGTPASG QSYDYKYDAT RYGWRTAVDY SWFGDQRAKA
310 320 330 340 350
NCDMLTKFFA RDGAKGIVDG YTIQGSKISN NHNASFIGPV AAASMTGYDL
360 370 380 390 400
NFAKELYRET VAVKDSEYYG YYGNSLRLLT LLYITGNFPN PLSDLSGQPT
410 420 430 440 450
PPSNPTPSLP PQVVYGDVNG DGNVNSTDLT MLKRYLLKSV TNINREAADV
460 470
NRDGAINSSD MTILKRYLIK SIPHLPY
Length:477
Mass (Da):52,594
Last modified:March 20, 2007 - v1
Checksum:iBA5A0AD5022E8A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03088 Genomic DNA Translation: AAA83521.1
CP000568 Genomic DNA Translation: ABN51508.1
PIRiA23100 CZCLAM
RefSeqiWP_003512420.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51508; ABN51508; Cthe_0269
GeneIDi35806170
KEGGicth:Cthe_0269

Similar proteinsi

Entry informationi

Entry nameiGUNA_CLOTH
AccessioniPrimary (citable) accession number: A3DC29
Secondary accession number(s): P04955
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health