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Protein

Endoglucanase A

Gene

celA

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Proton donor1
Active sitei152NucleophileCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16413.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Short name:
EGA
Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase
Gene namesi
Name:celA
Ordered Locus Names:Cthe_0269
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32By similarityAdd BLAST32
ChainiPRO_000028472133 – 477Endoglucanase AAdd BLAST445

Proteomic databases

PRIDEiA3DC29.

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0269.

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 44Combined sources3
Beta strandi48 – 50Combined sources3
Helixi52 – 70Combined sources19
Helixi86 – 88Combined sources3
Helixi94 – 106Combined sources13
Helixi110 – 121Combined sources12
Beta strandi128 – 130Combined sources3
Beta strandi132 – 134Combined sources3
Turni142 – 147Combined sources6
Helixi151 – 168Combined sources18
Beta strandi170 – 174Combined sources5
Helixi176 – 191Combined sources16
Turni194 – 196Combined sources3
Beta strandi201 – 205Combined sources5
Helixi213 – 215Combined sources3
Helixi218 – 228Combined sources11
Helixi232 – 247Combined sources16
Turni248 – 250Combined sources3
Beta strandi259 – 261Combined sources3
Beta strandi274 – 276Combined sources3
Helixi279 – 281Combined sources3
Helixi282 – 293Combined sources12
Helixi296 – 310Combined sources15
Helixi314 – 316Combined sources3
Turni334 – 336Combined sources3
Helixi337 – 344Combined sources8
Helixi350 – 362Combined sources13
Helixi367 – 369Combined sources3
Helixi372 – 384Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CEMX-ray1.65A33-395[»]
1IS9X-ray1.03A33-395[»]
1KWFX-ray0.94A33-395[»]
ProteinModelPortaliA3DC29.
SMRiA3DC29.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA3DC29.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini411 – 477DockerinPROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Contains 1 dockerin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F5Q. Bacteria.
ENOG410Y1JM. LUCA.
HOGENOMiHOG000069581.
OMAiMDIAYAL.
OrthoDBiPOG091H07QZ.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DC29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVKKRVGV VLLILAVLGV YMLAMPANTV SAAGVPFNTK YPYGPTSIAD
60 70 80 90 100
NQSEVTAMLK AEWEDWKSKR ITSNGAGGYK RVQRDASTNY DTVSEGMGYG
110 120 130 140 150
LLLAVCFNEQ ALFDDLYRYV KSHFNGNGLM HWHIDANNNV TSHDGGDGAA
160 170 180 190 200
TDADEDIALA LIFADKLWGS SGAINYGQEA RTLINNLYNH CVEHGSYVLK
210 220 230 240 250
PGDRWGGSSV TNPSYFAPAW YKVYAQYTGD TRWNQVADKC YQIVEEVKKY
260 270 280 290 300
NNGTGLVPDW CTASGTPASG QSYDYKYDAT RYGWRTAVDY SWFGDQRAKA
310 320 330 340 350
NCDMLTKFFA RDGAKGIVDG YTIQGSKISN NHNASFIGPV AAASMTGYDL
360 370 380 390 400
NFAKELYRET VAVKDSEYYG YYGNSLRLLT LLYITGNFPN PLSDLSGQPT
410 420 430 440 450
PPSNPTPSLP PQVVYGDVNG DGNVNSTDLT MLKRYLLKSV TNINREAADV
460 470
NRDGAINSSD MTILKRYLIK SIPHLPY
Length:477
Mass (Da):52,594
Last modified:March 20, 2007 - v1
Checksum:iBA5A0AD5022E8A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03088 Genomic DNA. Translation: AAA83521.1.
CP000568 Genomic DNA. Translation: ABN51508.1.
PIRiA23100. CZCLAM.
RefSeqiWP_003512420.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51508; ABN51508; Cthe_0269.
KEGGicth:Cthe_0269.
PATRICi19514173. VBICloThe47081_0284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03088 Genomic DNA. Translation: AAA83521.1.
CP000568 Genomic DNA. Translation: ABN51508.1.
PIRiA23100. CZCLAM.
RefSeqiWP_003512420.1. NC_009012.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CEMX-ray1.65A33-395[»]
1IS9X-ray1.03A33-395[»]
1KWFX-ray0.94A33-395[»]
ProteinModelPortaliA3DC29.
SMRiA3DC29.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_0269.

Protein family/group databases

CAZyiGH8. Glycoside Hydrolase Family 8.

Proteomic databases

PRIDEiA3DC29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN51508; ABN51508; Cthe_0269.
KEGGicth:Cthe_0269.
PATRICi19514173. VBICloThe47081_0284.

Phylogenomic databases

eggNOGiENOG4105F5Q. Bacteria.
ENOG410Y1JM. LUCA.
HOGENOMiHOG000069581.
OMAiMDIAYAL.
OrthoDBiPOG091H07QZ.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16413.

Miscellaneous databases

EvolutionaryTraceiA3DC29.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002037. Glyco_hydro_8.
IPR019834. Glyco_hydro_8_CS.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF01270. Glyco_hydro_8. 1 hit.
[Graphical view]
PRINTSiPR00735. GLHYDRLASE8.
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS00812. GLYCOSYL_HYDROL_F8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUNA_CLOTH
AccessioniPrimary (citable) accession number: A3DC29
Secondary accession number(s): P04955
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.