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A3DBX3

- GUB_CLOTH

UniProt

A3DBX3 - GUB_CLOTH

Protein

Beta-glucanase

Gene

licB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei136 – 1361NucleophilePROSITE-ProRule annotation
    Active sitei140 – 1401Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. licheninase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-216-MONOMER.
    MetaCyc:MONOMER-16465.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucanase (EC:3.2.1.73)
    Alternative name(s):
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Endo-beta-1,3-1,4 glucanase
    Laminarinase
    Lichenase
    Gene namesi
    Name:licB
    Synonyms:lam1
    Ordered Locus Names:Cthe_0211
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 334307Beta-glucanasePRO_0000284719Add
    BLAST

    Interactioni

    Subunit structurei

    May form part of a multienzyme complex (cellulosome).By similarity

    Protein-protein interaction databases

    STRINGi203119.Cthe_0211.

    Structurei

    3D structure databases

    ProteinModelPortaliA3DBX3.
    SMRiA3DBX3. Positions 41-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati273 – 296241Add
    BLAST
    Repeati308 – 331242Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni273 – 331592 X 24 AA approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi252 – 26918Pro/Thr-rich (linker)Add
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component By similarity.By similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000250723.
    OMAiTAPGEAC.
    OrthoDBiEOG6JB18P.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view]
    PfamiPF00404. Dockerin_1. 2 hits.
    PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 2 hits.
    PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A3DBX3-1 [UniParc]FASTAAdd to Basket

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    MKNRVISLLM ASLLLVLSVI VAPFYKAEAA TVVNTPFVAV FSNFDSSQWE    50
    KADWANGSVF NCVWKPSQVT FSNGKMILTL DREYGGSYPY KSGEYRTKSF 100
    FGYGYYEVRM KAAKNVGIVS SFFTYTGPSD NNPWDEIDIE FLGKDTTKVQ 150
    FNWYKNGVGG NEYLHNLGFD ASQDFHTYGF EWRPDYIDFY VDGKKVYRGT 200
    RNIPVTPGKI MMNLWPGIGV DEWLGRYDGR TPLQAEYEYV KYYPNGVPQD 250
    NPTPTPTIAP STPTNPNLPL KGDVNGDGHV NSSDYSLFKR YLLRVIDRFP 300
    VGDQSVADVN RDGRIDSTDL TMLKRYLIRA IPSL 334
    Length:334
    Mass (Da):37,897
    Last modified:March 20, 2007 - v1
    Checksum:i0837564E9726F281
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63355 Genomic DNA. Translation: CAA44959.1.
    CP000568 Genomic DNA. Translation: ABN51452.1.
    PIRiS23498.
    RefSeqiYP_001036645.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN51452; ABN51452; Cthe_0211.
    GeneIDi4808629.
    KEGGicth:Cthe_0211.
    PATRICi19514051. VBICloThe47081_0224.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63355 Genomic DNA. Translation: CAA44959.1 .
    CP000568 Genomic DNA. Translation: ABN51452.1 .
    PIRi S23498.
    RefSeqi YP_001036645.1. NC_009012.1.

    3D structure databases

    ProteinModelPortali A3DBX3.
    SMRi A3DBX3. Positions 41-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203119.Cthe_0211.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN51452 ; ABN51452 ; Cthe_0211 .
    GeneIDi 4808629.
    KEGGi cth:Cthe_0211.
    PATRICi 19514051. VBICloThe47081_0224.

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000250723.
    OMAi TAPGEAC.
    OrthoDBi EOG6JB18P.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-216-MONOMER.
    MetaCyc:MONOMER-16465.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view ]
    Pfami PF00404. Dockerin_1. 2 hits.
    PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 2 hits.
    PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the Clostridium thermocellum gene licB and the encoded beta-1,3-1,4-glucanase. A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases."
      Schimming S., Schwarz W.H., Staudenbauer W.L.
      Eur. J. Biochem. 204:13-19(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiGUB_CLOTH
    AccessioniPrimary (citable) accession number: A3DBX3
    Secondary accession number(s): P29716, P37074
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3