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A3DBX3

- GUB_CLOTH

UniProt

A3DBX3 - GUB_CLOTH

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Protein

Beta-glucanase

Gene

licB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei136 – 1361NucleophilePROSITE-ProRule annotation
Active sitei140 – 1401Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. licheninase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-216-MONOMER.
MetaCyc:MONOMER-16465.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Laminarinase
Lichenase
Gene namesi
Name:licB
Synonyms:lam1
Ordered Locus Names:Cthe_0211
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 334307Beta-glucanasePRO_0000284719Add
BLAST

Interactioni

Subunit structurei

May form part of a multienzyme complex (cellulosome).By similarity

Protein-protein interaction databases

STRINGi203119.Cthe_0211.

Structurei

3D structure databases

ProteinModelPortaliA3DBX3.
SMRiA3DBX3. Positions 41-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati273 – 296241Add
BLAST
Repeati308 – 331242Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 331592 X 24 AA approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi252 – 26918Pro/Thr-rich (linker)Add
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component (By similarity).By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000250723.
OMAiTAPGEAC.
OrthoDBiEOG6JB18P.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR013320. ConA-like_dom.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DBX3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNRVISLLM ASLLLVLSVI VAPFYKAEAA TVVNTPFVAV FSNFDSSQWE
60 70 80 90 100
KADWANGSVF NCVWKPSQVT FSNGKMILTL DREYGGSYPY KSGEYRTKSF
110 120 130 140 150
FGYGYYEVRM KAAKNVGIVS SFFTYTGPSD NNPWDEIDIE FLGKDTTKVQ
160 170 180 190 200
FNWYKNGVGG NEYLHNLGFD ASQDFHTYGF EWRPDYIDFY VDGKKVYRGT
210 220 230 240 250
RNIPVTPGKI MMNLWPGIGV DEWLGRYDGR TPLQAEYEYV KYYPNGVPQD
260 270 280 290 300
NPTPTPTIAP STPTNPNLPL KGDVNGDGHV NSSDYSLFKR YLLRVIDRFP
310 320 330
VGDQSVADVN RDGRIDSTDL TMLKRYLIRA IPSL
Length:334
Mass (Da):37,897
Last modified:March 20, 2007 - v1
Checksum:i0837564E9726F281
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63355 Genomic DNA. Translation: CAA44959.1.
CP000568 Genomic DNA. Translation: ABN51452.1.
PIRiS23498.
RefSeqiYP_001036645.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51452; ABN51452; Cthe_0211.
GeneIDi4808629.
KEGGicth:Cthe_0211.
PATRICi19514051. VBICloThe47081_0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63355 Genomic DNA. Translation: CAA44959.1 .
CP000568 Genomic DNA. Translation: ABN51452.1 .
PIRi S23498.
RefSeqi YP_001036645.1. NC_009012.1.

3D structure databases

ProteinModelPortali A3DBX3.
SMRi A3DBX3. Positions 41-245.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_0211.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN51452 ; ABN51452 ; Cthe_0211 .
GeneIDi 4808629.
KEGGi cth:Cthe_0211.
PATRICi 19514051. VBICloThe47081_0224.

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000250723.
OMAi TAPGEAC.
OrthoDBi EOG6JB18P.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-216-MONOMER.
MetaCyc:MONOMER-16465.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
2.60.120.200. 1 hit.
InterProi IPR008264. Beta_glucanase.
IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR013320. ConA-like_dom.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view ]
Pfami PF00404. Dockerin_1. 2 hits.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PRINTSi PR00737. GLHYDRLASE16.
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the Clostridium thermocellum gene licB and the encoded beta-1,3-1,4-glucanase. A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases."
    Schimming S., Schwarz W.H., Staudenbauer W.L.
    Eur. J. Biochem. 204:13-19(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiGUB_CLOTH
AccessioniPrimary (citable) accession number: A3DBX3
Secondary accession number(s): P29716, P37074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3