Reviewed, UniProtKB/Swiss-Prot A3DBX3 (GUB_CLOTH)

Names and origin

Protein names

Recommended name:
    Beta-glucanase
    EC=3.2.1.73
Alternative name(s):
    Endo-beta-1,3-1,4 glucanase
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase
    Laminarinase

Gene names

Name:	licB
Synonyms:	lam1
Ordered Locus Names:	Cthe_0211

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Subunit structure	May form part of a multienzyme complex (cellulosome) By similarity.
Domain	A 24 residues domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 334	307	Beta-glucanase		PRO_0000284719
Regions
	Repeat	273 – 296	24	1
	Repeat	308 – 331	24	2
	Region	273 – 331	59	2 X 24 AA approximate repeats
	Compositional bias	252 – 269	18	Pro/Thr-rich (linker)
Sites
	Active site	136	1	Nucleophile By similarity
	Active site	140	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3DBX3-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 0837564E9726F281	FASTA	334	37,897
	10 20 30 40 50 60 MKNRVISLLM ASLLLVLSVI VAPFYKAEAA TVVNTPFVAV FSNFDSSQWE KADWANGSVF 70 80 90 100 110 120 NCVWKPSQVT FSNGKMILTL DREYGGSYPY KSGEYRTKSF FGYGYYEVRM KAAKNVGIVS 130 140 150 160 170 180 SFFTYTGPSD NNPWDEIDIE FLGKDTTKVQ FNWYKNGVGG NEYLHNLGFD ASQDFHTYGF 190 200 210 220 230 240 EWRPDYIDFY VDGKKVYRGT RNIPVTPGKI MMNLWPGIGV DEWLGRYDGR TPLQAEYEYV 250 260 270 280 290 300 KYYPNGVPQD NPTPTPTIAP STPTNPNLPL KGDVNGDGHV NSSDYSLFKR YLLRVIDRFP 310 320 330 VGDQSVADVN RDGRIDSTDL TMLKRYLIRA IPSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the Clostridium thermocellum gene licB and the encoded beta-1,3-1,4-glucanase. A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases." Schimming S., Schwarz W.H., Staudenbauer W.L. Eur. J. Biochem. 204:13-19(1992) [PubMed: 1740123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63355 Genomic DNA. Translation: CAA44959.1. CP000568 Genomic DNA. Translation: ABN51452.1.
PIR	S23498.
RefSeq	YP_001036645.1.
3D structure databases
SMR	A3DBX3. Positions 41-245, 270-331.
ModBase	Search...
Protein-protein interaction databases
STRING	A3DBX3.
Genome annotation databases
GeneID	4808629.
GenomeReviews	Gene locus Cthe_0211 in contig CP000568_GR.
KEGG	cth:Cthe_0211.
NMPDR	fig|203119.1.peg.2310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2273.
HOGENOM	HBG338471.
OMA	NIFAHGK.
Family and domain databases
InterPro	IPR008264. Beta_glucanase. IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR018242. Dockerin_1. IPR018247. EF_Hand_1_Ca_BS. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Gene3D	G3DSA:1.10.1330.10. Cellulos_enz_dockerin_1. 1 hit. G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00404. Dockerin_1. 2 hits. PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PRINTS	PR00737. GLHYDRLASE16.
PROSITE	PS00448. CLOS_CELLULOSOME_RPT. 2 hits. PS00018. EF_HAND_1. 2 hits. Uncertain. PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_CLOTH

Accession

Primary (citable) accession number: A3DBX3
Secondary accession number(s): P29716, P37074

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	March 20, 2007
Last modified:	January 19, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)