Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-glucanase

Gene

licB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei136NucleophilePROSITE-ProRule annotation1
Active sitei140Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-220-MONOMER
MetaCyc:MONOMER-16465

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Laminarinase
Lichenase
Gene namesi
Name:licB
Synonyms:lam1
Ordered Locus Names:Cthe_0211
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000028471928 – 334Beta-glucanaseAdd BLAST307

Interactioni

Subunit structurei

May form part of a multienzyme complex (cellulosome).By similarity

Protein-protein interaction databases

STRINGi203119.Cthe_0211

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 40Combined sources4
Helixi46 – 48Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi62 – 64Combined sources3
Helixi66 – 68Combined sources3
Beta strandi69 – 72Combined sources4
Beta strandi75 – 82Combined sources8
Beta strandi84 – 92Combined sources9
Beta strandi94 – 101Combined sources8
Beta strandi103 – 111Combined sources9
Beta strandi118 – 126Combined sources9
Helixi128 – 130Combined sources3
Beta strandi135 – 142Combined sources8
Beta strandi148 – 155Combined sources8
Beta strandi163 – 166Combined sources4
Turni171 – 173Combined sources3
Beta strandi176 – 183Combined sources8
Beta strandi186 – 191Combined sources6
Beta strandi194 – 199Combined sources6
Beta strandi208 – 219Combined sources12
Helixi221 – 224Combined sources4
Beta strandi233 – 245Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WVJX-ray1.95A/B30-249[»]
ProteinModelPortaliA3DBX3
SMRiA3DBX3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 248GH16PROSITE-ProRule annotationAdd BLAST221
Domaini267 – 334DockerinPROSITE-ProRule annotationAdd BLAST68

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 269Pro/Thr-rich (linker)Add BLAST18

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108RZ2 Bacteria
COG2273 LUCA
HOGENOMiHOG000250723
OMAiWYVSDGW
OrthoDBiPOG091H03QV

Family and domain databases

InterProiView protein in InterPro
IPR008264 Beta_glucanase
IPR013320 ConA-like_dom_sf
IPR002105 Dockerin_1_rpt
IPR016134 Dockerin_dom
IPR036439 Dockerin_dom_sf
IPR018247 EF_Hand_1_Ca_BS
IPR000757 GH16
IPR008263 GH16_AS
PfamiView protein in Pfam
PF00404 Dockerin_1, 2 hits
PF00722 Glyco_hydro_16, 1 hit
PRINTSiPR00737 GLHYDRLASE16
SUPFAMiSSF49899 SSF49899, 1 hit
SSF63446 SSF63446, 1 hit
PROSITEiView protein in PROSITE
PS00448 CLOS_CELLULOSOME_RPT, 2 hits
PS51766 DOCKERIN, 1 hit
PS00018 EF_HAND_1, 2 hits
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DBX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNRVISLLM ASLLLVLSVI VAPFYKAEAA TVVNTPFVAV FSNFDSSQWE
60 70 80 90 100
KADWANGSVF NCVWKPSQVT FSNGKMILTL DREYGGSYPY KSGEYRTKSF
110 120 130 140 150
FGYGYYEVRM KAAKNVGIVS SFFTYTGPSD NNPWDEIDIE FLGKDTTKVQ
160 170 180 190 200
FNWYKNGVGG NEYLHNLGFD ASQDFHTYGF EWRPDYIDFY VDGKKVYRGT
210 220 230 240 250
RNIPVTPGKI MMNLWPGIGV DEWLGRYDGR TPLQAEYEYV KYYPNGVPQD
260 270 280 290 300
NPTPTPTIAP STPTNPNLPL KGDVNGDGHV NSSDYSLFKR YLLRVIDRFP
310 320 330
VGDQSVADVN RDGRIDSTDL TMLKRYLIRA IPSL
Length:334
Mass (Da):37,897
Last modified:March 20, 2007 - v1
Checksum:i0837564E9726F281
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63355 Genomic DNA Translation: CAA44959.1
CP000568 Genomic DNA Translation: ABN51452.1
PIRiS23498
RefSeqiWP_003512300.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51452; ABN51452; Cthe_0211
GeneIDi35805480
KEGGicth:Cthe_0211

Similar proteinsi

Entry informationi

Entry nameiGUB_CLOTH
AccessioniPrimary (citable) accession number: A3DBX3
Secondary accession number(s): P29716, P37074
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health