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Protein

Beta-glucanase

Gene

licB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei136NucleophilePROSITE-ProRule annotation1
Active sitei140Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16465.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Laminarinase
Lichenase
Gene namesi
Name:licB
Synonyms:lam1
Ordered Locus Names:Cthe_0211
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000028471928 – 334Beta-glucanaseAdd BLAST307

Interactioni

Subunit structurei

May form part of a multienzyme complex (cellulosome).By similarity

Protein-protein interaction databases

STRINGi203119.Cthe_0211.

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 40Combined sources4
Helixi46 – 48Combined sources3
Beta strandi49 – 51Combined sources3
Beta strandi62 – 64Combined sources3
Helixi66 – 68Combined sources3
Beta strandi69 – 72Combined sources4
Beta strandi75 – 82Combined sources8
Beta strandi84 – 92Combined sources9
Beta strandi94 – 101Combined sources8
Beta strandi103 – 111Combined sources9
Beta strandi118 – 126Combined sources9
Helixi128 – 130Combined sources3
Beta strandi135 – 142Combined sources8
Beta strandi148 – 155Combined sources8
Beta strandi163 – 166Combined sources4
Turni171 – 173Combined sources3
Beta strandi176 – 183Combined sources8
Beta strandi186 – 191Combined sources6
Beta strandi194 – 199Combined sources6
Beta strandi208 – 219Combined sources12
Helixi221 – 224Combined sources4
Beta strandi233 – 245Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WVJX-ray1.95A/B30-249[»]
ProteinModelPortaliA3DBX3.
SMRiA3DBX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 248GH16PROSITE-ProRule annotationAdd BLAST221
Domaini267 – 334DockerinPROSITE-ProRule annotationAdd BLAST68

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 269Pro/Thr-rich (linker)Add BLAST18

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 dockerin domain.PROSITE-ProRule annotation
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108RZ2. Bacteria.
COG2273. LUCA.
HOGENOMiHOG000250723.
OMAiTAPGEAC.
OrthoDBiPOG091H03QV.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A3DBX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNRVISLLM ASLLLVLSVI VAPFYKAEAA TVVNTPFVAV FSNFDSSQWE
60 70 80 90 100
KADWANGSVF NCVWKPSQVT FSNGKMILTL DREYGGSYPY KSGEYRTKSF
110 120 130 140 150
FGYGYYEVRM KAAKNVGIVS SFFTYTGPSD NNPWDEIDIE FLGKDTTKVQ
160 170 180 190 200
FNWYKNGVGG NEYLHNLGFD ASQDFHTYGF EWRPDYIDFY VDGKKVYRGT
210 220 230 240 250
RNIPVTPGKI MMNLWPGIGV DEWLGRYDGR TPLQAEYEYV KYYPNGVPQD
260 270 280 290 300
NPTPTPTIAP STPTNPNLPL KGDVNGDGHV NSSDYSLFKR YLLRVIDRFP
310 320 330
VGDQSVADVN RDGRIDSTDL TMLKRYLIRA IPSL
Length:334
Mass (Da):37,897
Last modified:March 20, 2007 - v1
Checksum:i0837564E9726F281
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63355 Genomic DNA. Translation: CAA44959.1.
CP000568 Genomic DNA. Translation: ABN51452.1.
PIRiS23498.
RefSeqiWP_003512300.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51452; ABN51452; Cthe_0211.
KEGGicth:Cthe_0211.
PATRICi19514051. VBICloThe47081_0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63355 Genomic DNA. Translation: CAA44959.1.
CP000568 Genomic DNA. Translation: ABN51452.1.
PIRiS23498.
RefSeqiWP_003512300.1. NC_009012.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WVJX-ray1.95A/B30-249[»]
ProteinModelPortaliA3DBX3.
SMRiA3DBX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_0211.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN51452; ABN51452; Cthe_0211.
KEGGicth:Cthe_0211.
PATRICi19514051. VBICloThe47081_0224.

Phylogenomic databases

eggNOGiENOG4108RZ2. Bacteria.
COG2273. LUCA.
HOGENOMiHOG000250723.
OMAiTAPGEAC.
OrthoDBiPOG091H03QV.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16465.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00404. Dockerin_1. 2 hits.
PF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUB_CLOTH
AccessioniPrimary (citable) accession number: A3DBX3
Secondary accession number(s): P29716, P37074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 20, 2007
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.