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Protein

Argininosuccinate synthase

Gene

argG

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei88CitrullineUniRule annotation1
Binding sitei93CitrullineUniRule annotation1
Binding sitei118ATP; via amide nitrogenUniRule annotation1
Binding sitei120AspartateUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei124CitrullineUniRule annotation1
Binding sitei125AspartateUniRule annotation1
Binding sitei128CitrullineUniRule annotation1
Binding sitei177CitrullineUniRule annotation1
Binding sitei186CitrullineUniRule annotation1
Binding sitei263CitrullineUniRule annotation1
Binding sitei275CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-186-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Cthe_0179
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003213091 – 405Argininosuccinate synthaseAdd BLAST405

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi203119.Cthe_0179

Structurei

3D structure databases

ProteinModelPortaliA3DBU1
SMRiA3DBU1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM
OrthoDBiPOG091H01U0

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.90.1260.10, 1 hit
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

A3DBU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKEKVILA YSGGLDTSII IPWLKENYDY EVIAMAADVG QGEELEPLRE
60 70 80 90 100
KAIKTGASKI YIEDLKEEFV TDFIFPTLKA GAVYEGKYLL GTSFARPLIA
110 120 130 140 150
KRMVEIALKE GATAVAHGAT GKGNDQVRFE LTVKALAPHL KIIAPWRIWD
160 170 180 190 200
IKSREDEIEY AQARNIPIPV SKEDNYSMDR NLWHLSHEGL DLEDPWNEPQ
210 220 230 240 250
YDKILKLMVP PEKAPDKPTY VEIYFEKGIP KKVNGVEYGP VELIEVLNKI
260 270 280 290 300
GGENGIGIVD IVENRLVGMK SRGVYETPGG TILYAAHREL ELLCLDRDTL
310 320 330 340 350
HYKDLVAQRF AELVYYGQWY TPLREAISAF VDVTQETVTG TVRLKLYKGN
360 370 380 390 400
IISAGAKSDY SLYSEELSTF GEDNVYNQKD AEGFINLFGL PMKVQALMKE

KNKGK
Length:405
Mass (Da):45,622
Last modified:March 20, 2007 - v1
Checksum:i55DD311637177784
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN51420.1
RefSeqiWP_003512250.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51420; ABN51420; Cthe_0179
GeneIDi35805053
KEGGicth:Cthe_0179

Similar proteinsi

Entry informationi

Entry nameiASSY_CLOTH
AccessioniPrimary (citable) accession number: A3DBU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health