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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43S-adenosyl-L-methionineUniRule annotation1
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei101S-adenosyl-L-methionineUniRule annotation1
Binding sitei124S-adenosyl-L-methionineUniRule annotation1
Binding sitei128SubstrateUniRule annotation1
Binding sitei160SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCTHE203119:G1G86-106-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Cthe_0102
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881421 – 218tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST218

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0102

Structurei

3D structure databases

ProteinModelPortaliA3DBL4
SMRiA3DBL4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000251689
KOiK03439
OMAiWITFPDP
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR022272 Lipocalin_CS
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A3DBL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRKKPWAR PALEACSFFV INPTEYKGKW REVFGNSNEI WLELGCGKGG
60 70 80 90 100
FISKLASTNP DKNFIAVDIK DEVLALAMKK IENEYALIGA ETKNIRLMAH
110 120 130 140 150
EIMLIHRMLD ENDQIGRIFI NFCNPWPKNR HKARRLTHPN QLNQYRTFLA
160 170 180 190 200
PNGQIWFKTD DTMLFQDSIK YFEQCNFNIV YLTEDLHASG FEGNIETEHE
210
RMFLEQGCKI KFLIAEKK
Length:218
Mass (Da):25,550
Last modified:March 20, 2007 - v1
Checksum:i6AC2B754C1F2485F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000568 Genomic DNA Translation: ABN51343.1
RefSeqiWP_003512099.1, NC_009012.1

Genome annotation databases

EnsemblBacteriaiABN51343; ABN51343; Cthe_0102
GeneIDi35803802
KEGGicth:Cthe_0102

Similar proteinsi

Entry informationi

Entry nameiTRMB_CLOTH
AccessioniPrimary (citable) accession number: A3DBL4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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