Reviewed, UniProtKB/Swiss-Prot A3DBI2 (SYN_CLOTH)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Cthe_0070

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000051387

Sequences

	Sequence		Length	Mass (Da)
	A3DBI2-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 144AAD7751D2EDE2	FASTA	464	53,526
	10 20 30 40 50 60 MKTTLVKSLY RNTKDYVGKE IVVAGWVRTV RDSKAFGFIE LNDGSFFKNL QIVFEEKNLP 70 80 90 100 110 120 NFKEIAKLSV GSAIIAHGEL VETPGAKQPF ELKATKIEIE GASTPDYPLQ KKRHSFEYLR 130 140 150 160 170 180 TIAHLRPRTN TFSAVFRVRS LLAYAIHKFF QERGFVYVHT PIITGSDAEG AGQMFKVTTL 190 200 210 220 230 240 DINNPPRKED GTVDFSKDFF DRETNLTVSG QLEGETYSMA FRNIYTFGPT FRAENSNTAR 250 260 270 280 290 300 HAAEFWMVEP EIAFADLEDD MELAEDMLKY IINYCLENAP EEMEFFNNFI DNTLLDRLNN 310 320 330 340 350 360 IVSSDFAHVT YTEAIDILSK ADQKFEYPVK WGNDLQTEHE RYLTEKVFKK PVFVTDYPKD 370 380 390 400 410 420 IKAFYMRLND DNKTVAAMDL LVPGVGEIIG GSQREERLDY LENRMKELGL KMEDYWWYLD 430 440 450 460 LRRYGSTRHA GFGLGFERAI MYITGMSNIR DVIPFPRTVH SAEF

References

[1]

"Complete sequence of Clostridium thermocellum ATCC 27405." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000568 Genomic DNA. Translation: ABN51311.1.
RefSeq	YP_001036504.1.
3D structure databases
SMR	A3DBI2. Positions 13-462.
ModBase	Search...
Protein-protein interaction databases
STRING	A3DBI2.
Genome annotation databases
GeneID	4808765.
GenomeReviews	Gene locus Cthe_0070 in contig CP000568_GR.
KEGG	cth:Cthe_0070.
NMPDR	fig|203119.1.peg.846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOTH

Accession

Primary (citable) accession number: A3DBI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 20, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)