UniProtKB - A3DBD3 (BIOB_CLOTH)
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Protein
Biotin synthase
Gene
bioB
Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Functioni
Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation
Catalytic activityi
Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
- [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation
: biotin biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- ATP-dependent dethiobiotin synthetase BioD (bioD)
- Biotin synthase (bioB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 68 | Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 71 | Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 108 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 140 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 200 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 270 | Iron-sulfur 2 (2Fe-2S)UniRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- biotin synthase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- biotin biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Biotin biosynthesis |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | CTHE203119:G1G86-20-MONOMER. |
UniPathwayi | UPA00078; UER00162. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:bioBUniRule annotation Ordered Locus Names:Cthe_0020 |
Organismi | Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum) |
Taxonomic identifieri | 203119 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ruminiclostridium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000381323 | 1 – 320 | Biotin synthaseAdd BLAST | 320 |
Proteomic databases
PRIDEi | A3DBD3. |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 203119.Cthe_0020. |
Structurei
3D structure databases
ProteinModelPortali | A3DBD3. |
SMRi | A3DBD3. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105CZF. Bacteria. COG0502. LUCA. |
HOGENOMi | HOG000239958. |
KOi | K01012. |
OMAi | ADRFCMG. |
OrthoDBi | POG091H01DF. |
Family and domain databases
Gene3Di | 3.20.20.70. 1 hit. |
HAMAPi | MF_01694. BioB. 1 hit. |
InterProi | View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. |
PANTHERi | PTHR22976. PTHR22976. 1 hit. |
Pfami | View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. |
PIRSFi | PIRSF001619. Biotin_synth. 1 hit. |
SFLDi | SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit. |
SMARTi | View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. |
TIGRFAMsi | TIGR00433. bioB. 1 hit. |
i Sequence
Sequence statusi: Complete.
A3DBD3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFLTLMENK LDEGRMITFE EAVELAKGKI EDEKLFLLAD KLCKKNMGRR
60 70 80 90 100
VDLCSIINAK SGGCSENCKF CAQSGHYDTG VKIYPLLDVD DVLKAAKENE
110 120 130 140 150
KEGVHRFSLV TSGKSVSDEE FEKILGIYSV LRKETNLKLC ASLGSLSYER
160 170 180 190 200
AVRLKEAGVS MYHHNIETCR EYYPKICDTH TYDDRINTVK NAAKAGLEIC
210 220 230 240 250
CGGIIGMGES MEQRIKMAFE IRELGVKSVP INVLNPIKGT PFENVRSLSP
260 270 280 290 300
DEILRTIALF RLIMPYAHIR YAGGRMCLGE HQSKGFKAGV SAMLVGNYLT
310 320
TVGNKISDDL EMIQRMGLEI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000568 Genomic DNA. Translation: ABN51262.1. |
RefSeqi | WP_003511922.1. NC_009012.1. |
Genome annotation databases
EnsemblBacteriai | ABN51262; ABN51262; Cthe_0020. |
KEGGi | cth:Cthe_0020. |
Similar proteinsi
Entry informationi
Entry namei | BIOB_CLOTH | |
Accessioni | A3DBD3Primary (citable) accession number: A3DBD3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 28, 2009 |
Last sequence update: | March 20, 2007 | |
Last modified: | February 28, 2018 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |