bioB

Functionⁱ

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityⁱ

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactorⁱ

Protein has several cofactor binding sites:

[4Fe-4S] cluster
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
[2Fe-2S] cluster
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694
Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01694

Pathwayⁱ: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

ATP-dependent dethiobiotin synthetase BioD (bioD)
Biotin synthase (bioB)

This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	64	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	68	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	71	Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation	1
Metal bindingⁱ	108	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	140	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	200	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1
Metal bindingⁱ	270	Iron-sulfur 2 (2Fe-2S)UniRule annotation	1

GO - Molecular functionⁱ

2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
biotin synthase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

biotin biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Biotin biosynthesis
Ligand	2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	CTHE203119:G1G86-20-MONOMER.
UniPathwayⁱ	UPA00078; UER00162.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesⁱ	Name:bioBUniRule annotation Ordered Locus Names:Cthe_0020
Organismⁱ	Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifierⁱ	203119 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ruminiclostridium › Clostridium thermocellum
Proteomesⁱ	UP000002145 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000381323	1 – 320	Biotin synthaseAdd BLAST		320

Proteomic databases

PRIDEⁱ	A3DBD3.

PRIDEⁱ

A3DBD3.

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	203119.Cthe_0020.

STRINGⁱ

203119.Cthe_0020.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	A3DBD3.
SMRⁱ	A3DBD3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105CZF. Bacteria. COG0502. LUCA.
HOGENOMⁱ	HOG000239958.
KEGG Orthology (KO) More...KOⁱ	K01012.
OMAⁱ	ADRFCMG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01DF.

Family and domain databases

Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_01694. BioB. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM.
PANTHERⁱ	PTHR22976. PTHR22976. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit.
PIRSFⁱ	PIRSF001619. Biotin_synth. 1 hit.
SFLDⁱ	SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit.
SMARTⁱ	View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit.
TIGRFAMsⁱ	TIGR00433. bioB. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

A3DBD3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDFLTLMENK LDEGRMITFE EAVELAKGKI EDEKLFLLAD KLCKKNMGRR 
        60         70         80         90        100
VDLCSIINAK SGGCSENCKF CAQSGHYDTG VKIYPLLDVD DVLKAAKENE 
       110        120        130        140        150
KEGVHRFSLV TSGKSVSDEE FEKILGIYSV LRKETNLKLC ASLGSLSYER 
       160        170        180        190        200
AVRLKEAGVS MYHHNIETCR EYYPKICDTH TYDDRINTVK NAAKAGLEIC 
       210        220        230        240        250
CGGIIGMGES MEQRIKMAFE IRELGVKSVP INVLNPIKGT PFENVRSLSP 
       260        270        280        290        300
DEILRTIALF RLIMPYAHIR YAGGRMCLGE HQSKGFKAGV SAMLVGNYLT 
       310        320
TVGNKISDDL EMIQRMGLEI

Length:320

Mass (Da):35,743

Last modified:March 20, 2007 - v1

Checksum:ⁱBC7DA44046DA398F

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000568 Genomic DNA. Translation: ABN51262.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003511922.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaⁱ	ABN51262; ABN51262; Cthe_0020.
KEGGⁱ	cth:Cthe_0020.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A3DBD3	UPI0001B1F02D B9E2A2 A5N8V7 A0A1L5F8K8 G8LVA4 A0A037Z8Q0 K0AWM1 A0A1M6VVV4 UPI000513A523 U6ERG2 +7	Clostridium thermocellum (Ruminiclostridium thermocellum) Clostridium kluyveri (strain NBRC 12016) Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) Clostridium kluyveri Clostridium clariflavum (strain DSM 19732 / NBRC 101661 / EBR45) Clostridium tetanomorphum DSM 665 Clostridium acidurici (strain ATCC 7906 / DSM 604 / BCRC 14475 / CIP 104303 / NCIMB 10678 / 9a) (Gottschalkia acidurici) Desulfotomaculum aeronauticum DSM 10349 Clostridium tetani Clostridium tetani 12124569 And more	320	UniRef50_A3DBD3	Cluster: Biotin synthase	18

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000568 Genomic DNA. Translation: ABN51262.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_003511922.1. NC_009012.1.

3D structure databases

ProteinModelPortalⁱ	A3DBD3.
SMRⁱ	A3DBD3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	203119.Cthe_0020.

STRINGⁱ

203119.Cthe_0020.

Proteomic databases

PRIDEⁱ	A3DBD3.

PRIDEⁱ

A3DBD3.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABN51262; ABN51262; Cthe_0020.
KEGGⁱ	cth:Cthe_0020.

Phylogenomic databases

eggNOGⁱ	ENOG4105CZF. Bacteria. COG0502. LUCA.
HOGENOMⁱ	HOG000239958.
KEGG Orthology (KO) More...KOⁱ	K01012.
OMAⁱ	ADRFCMG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01DF.

Enzyme and pathway databases

UniPathwayⁱ	UPA00078; UER00162.
BioCycⁱ	CTHE203119:G1G86-20-MONOMER.

Family and domain databases

Gene3Dⁱ	3.20.20.70. 1 hit.
HAMAPⁱ	MF_01694. BioB. 1 hit.
InterProⁱ	View protein in InterPro IPR013785. Aldolase_TIM. IPR010722. BATS_dom. IPR034416. BATS_domain_containing. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM.
PANTHERⁱ	PTHR22976. PTHR22976. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit.
PIRSFⁱ	PIRSF001619. Biotin_synth. 1 hit.
SFLDⁱ	SFLDG01060. BATS_domain_containing. 1 hit. SFLDG01278. biotin_synthase_like. 1 hit. SFLDS00029. Radical_SAM. 1 hit.
SMARTⁱ	View protein in SMART SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit.
TIGRFAMsⁱ	TIGR00433. bioB. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BIOB_CLOTH
Accessionⁱ	A3DBD3Primary (citable) accession number: A3DBD3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
	Last sequence update:	March 20, 2007
	Last modified:	February 28, 2018
	This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A3DBD3 (BIOB_CLOTH)

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Biotin synthase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: biotin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: biotin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ