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A3D7P9

- LIPA_SHEB5

UniProt

A3D7P9 - LIPA_SHEB5

Protein

Lipoyl synthase

Gene

lipA

Organism
Shewanella baltica (strain OS155 / ATCC BAA-1091)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSBAL325240:GCTA-3388-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Sbal_3282
    OrganismiShewanella baltica (strain OS155 / ATCC BAA-1091)
    Taxonomic identifieri325240 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000001557: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321Lipoyl synthasePRO_1000012272Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi325240.Sbal_3282.

    Structurei

    3D structure databases

    ProteinModelPortaliA3D7P9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A3D7P9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRPERLQPG VKLRDADKVS RIPVKIVPSE RDTMLRKPDW LRVKLPASNQ    50
    RILEIKQALR KNGLHSVCEE ASCPNLAECF NHGTATFMIL GAICTRRCPF 100
    CDVAHGRPLK PDAEEPVKLA QTIRDMKLKY VVITSVDRDD LRDGGAQHFA 150
    DCIREIRKLN PDIKIETLVP DFRGRIDAAL DILSTEPPDV FNHNLETAPM 200
    HYRKARPGAN YQWSLDLLKR FKERHPNVPT KSGLMMGLGE TNEEIAQVLR 250
    DLRAHNVEML TLGQYLQPSK FHLPVERYVP PAEFDELKAL ADELGFTHAA 300
    CGPLVRSSYH ADLQAQGKEV K 321
    Length:321
    Mass (Da):36,376
    Last modified:March 20, 2007 - v1
    Checksum:iE2B1C9A4F1F2F056
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000563 Genomic DNA. Translation: ABN62762.1.
    RefSeqiYP_001051631.1. NC_009052.1.

    Genome annotation databases

    EnsemblBacteriaiABN62762; ABN62762; Sbal_3282.
    GeneIDi4844038.
    KEGGisbl:Sbal_3282.
    PATRICi37185776. VBISheBal55297_3654.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000563 Genomic DNA. Translation: ABN62762.1 .
    RefSeqi YP_001051631.1. NC_009052.1.

    3D structure databases

    ProteinModelPortali A3D7P9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 325240.Sbal_3282.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN62762 ; ABN62762 ; Sbal_3282 .
    GeneIDi 4844038.
    KEGGi sbl:Sbal_3282.
    PATRICi 37185776. VBISheBal55297_3654.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci SBAL325240:GCTA-3388-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OS155 / ATCC BAA-1091.

    Entry informationi

    Entry nameiLIPA_SHEB5
    AccessioniPrimary (citable) accession number: A3D7P9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3