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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Shewanella baltica (strain OS155 / ATCC BAA-1091)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSBAL325240:GCTA-3375-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Sbal_3269
OrganismiShewanella baltica (strain OS155 / ATCC BAA-1091)
Taxonomic identifieri325240 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001557 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 859859Leucine--tRNA ligasePRO_0000334813Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi325240.Sbal_3269.

Structurei

3D structure databases

ProteinModelPortaliA3D7N6.
SMRiA3D7N6. Positions 228-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3D7N6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYNPSEI EALVQKHWHD NKTFEVTEDA NKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVARFQRLQG KNVLQPIGWD SFGLPAENAA INNKTAPAPW
110 120 130 140 150
TYENIEYMKN QLKLLGFGYD WSREIATCTP EYYRWEQWFF TKLYEKGLVY
160 170 180 190 200
KKTASVNWCP NDETVLANEQ VQDGCCWRCD TPVEQKEIPQ WFIKITAYAE
210 220 230 240 250
ELLNDIDTLD GWPDQVKTMQ RNWIGRSEGV EMTFGVAGHD KSFDIYTTRP
260 270 280 290 300
DTLMGVTYVA IAAGHPLAEI AAHTNPELAA FIDECKNSTT SEAELATMEK
310 320 330 340 350
RGVATGLFAI HPITGKQVPI WAANFVLMNY GTGAVMSVPG HDQRDFEFAK
360 370 380 390 400
KYGLAIEAVI KPVDGDVDIS EAAYTEKGVL FNSGEFDGLD FEAGFNAIAN
410 420 430 440 450
KLVAEGKGKR QVNYRLRDWG VSRQRYWGAP IPMVTLADGT VIPTPADQLP
460 470 480 490 500
VLLPEDVVMD GIQSPIKADK EWAKTQVNGQ DALRETDTFD TFMESSWYYA
510 520 530 540 550
RYCSPHADEM LDPAKANYWL PVDQYIGGIE HACMHLLYFR FFHKLLRDAG
560 570 580 590 600
LVNSNEPAKQ LLTQGMVLAD AFYYINEKGA RVWVSPLDVA TTEKDDKGRI
610 620 630 640 650
TKAIDKDGNE LVYTGMSKMS KSKNNGIDPQ VMVEKYGADT VRLFMMFASP
660 670 680 690 700
PELTLEWQES GVEGAHRFIK RLWKLANEHV NQDNSEALDV STLTSDQKAL
710 720 730 740 750
RREVHKTIAK VTDDIGRRQM FNTAVAAVME LMNHLQKAPQ TTGQDNAIIG
760 770 780 790 800
EALSAIVRLL YPIIPHVSFN LWNELGNASN IEDSQWPVVD EAALVEDSKL
810 820 830 840 850
IVVQVNGKVR AKITVAADAD KESVEALGMS DEHVIKYLDG LTVRKVIYVP

GKLLSIVAN
Length:859
Mass (Da):96,606
Last modified:May 19, 2008 - v2
Checksum:i819251F1C94B704B
GO

Sequence cautioni

The sequence ABN62749.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000563 Genomic DNA. Translation: ABN62749.1. Different initiation.
RefSeqiYP_001051618.1. NC_009052.1.

Genome annotation databases

EnsemblBacteriaiABN62749; ABN62749; Sbal_3269.
KEGGisbl:Sbal_3269.
PATRICi37185750. VBISheBal55297_3641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000563 Genomic DNA. Translation: ABN62749.1. Different initiation.
RefSeqiYP_001051618.1. NC_009052.1.

3D structure databases

ProteinModelPortaliA3D7N6.
SMRiA3D7N6. Positions 228-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi325240.Sbal_3269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN62749; ABN62749; Sbal_3269.
KEGGisbl:Sbal_3269.
PATRICi37185750. VBISheBal55297_3641.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSBAL325240:GCTA-3375-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS155 / ATCC BAA-1091.

Entry informationi

Entry nameiSYL_SHEB5
AccessioniPrimary (citable) accession number: A3D7N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 19, 2008
Last sequence update: May 19, 2008
Last modified: March 31, 2015
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.