Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella baltica (strain OS155 / ATCC BAA-1091)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathway:ifatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1161Important for catalytic activityUniRule annotation
Sitei138 – 1381Important for catalytic activityUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSBAL325240:GCTA-2831-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:Sbal_2760
OrganismiShewanella baltica (strain OS155 / ATCC BAA-1091)
Taxonomic identifieri325240 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001557 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Fatty acid oxidation complex subunit alphaPRO_0000323526Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi325240.Sbal_2760.

Structurei

3D structure databases

ProteinModelPortaliA3D684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 188188Enoyl-CoA hydrataseUniRule annotationAdd
BLAST
Regioni308 – 7063993-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.

Sequencei

Sequence statusi: Complete.

A3D684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKTFNLTRR DDGIAILTMD VPGETMNTLK AQFGPEISEI LADIKSDSSI
60 70 80 90 100
RGLVLISGKK DSFVAGADIS MLDACKTAGD AKALSQQGHV VFNELEALTI
110 120 130 140 150
PVVAAIHGAC LGGGLELALA CHQRVCSDDG KTMLGVPEVQ LGLLPGGGGT
160 170 180 190 200
QRLPRLVGIT TALDMMLTGK QIRPKQALKM GLVNDVVPQT ILLQTAVEMA
210 220 230 240 250
LAGKRAPKPV KKSLVNQVLE GTSFGRNIIF DQATKQVEKK TQGNYPAPAK
260 270 280 290 300
IIDCVRQGIT KGMQKGLEVE ASHFAELVVS KESEALRSIF FATTEMKKET
310 320 330 340 350
GAEGASPRKV KKAVILGGGL MGGGIASVTT TKAKIPVRVK DISEKGLSNA
360 370 380 390 400
LAYAYKLLDK GVKRRHMTPA ARDNLMALMT TTTEYKGVKD ADIVVEAVFE
410 420 430 440 450
DLALKHQMVK DIERECGEHT IFASNTSSLP ISQIAEAATR PENVIGLHYF
460 470 480 490 500
SPVEKMPLVE VIAHAKTSPE TIATTVAFAR KQGKTPIVVQ DGAGFYVNRI
510 520 530 540 550
LALYMNEAAQ LLLEGQSVEH LDKALVKFGF PVGPITLLDE VGIDVGAKIS
560 570 580 590 600
PILEKELGER FKAPAAFDKL LGDDRKGRKN GKGFYQYGAS SKKAKAVDES
610 620 630 640 650
VYGVLGIKPG TNKDAKALAE RCVVQMLNEA VRCLDDGIIA SPRDGDIGAI
660 670 680 690 700
FGIGFPPFLG GPFHYIDTLG AANLVRILEG YQSQLGSRFE PCERLKTMAQ

ENAHFF
Length:706
Mass (Da):75,726
Last modified:March 20, 2007 - v1
Checksum:i39592DC283F9D7D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000563 Genomic DNA. Translation: ABN62247.1.
RefSeqiWP_011847190.1. NC_009052.1.

Genome annotation databases

EnsemblBacteriaiABN62247; ABN62247; Sbal_2760.
KEGGisbl:Sbal_2760.
PATRICi37184602. VBISheBal55297_3106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000563 Genomic DNA. Translation: ABN62247.1.
RefSeqiWP_011847190.1. NC_009052.1.

3D structure databases

ProteinModelPortaliA3D684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi325240.Sbal_2760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN62247; ABN62247; Sbal_2760.
KEGGisbl:Sbal_2760.
PATRICi37184602. VBISheBal55297_3106.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiMMMLNEA.
OrthoDBiEOG6M9F0M.

Enzyme and pathway databases

UniPathwayiUPA00659.
BioCyciSBAL325240:GCTA-2831-MONOMER.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OS155 / ATCC BAA-1091.

Entry informationi

Entry nameiFADJ_SHEB5
AccessioniPrimary (citable) accession number: A3D684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 20, 2007
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.