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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno), Enolase (eno)
  5. Pyruvate kinase (Memar_0527)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110NAD; via amide nitrogenUniRule annotation1
Active sitei140NucleophileUniRule annotation1
Binding sitei168NADUniRule annotation1
Binding sitei302NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDHUniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gapUniRule annotation
Ordered Locus Names:Memar_2497
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003009731 – 341Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST341

Proteomic databases

PRIDEiA3CYG8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_2497.

Structurei

3D structure databases

ProteinModelPortaliA3CYG8.
SMRiA3CYG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni139 – 141Glyceraldehyde 3-phosphate bindingUniRule annotation3
Regioni194 – 195Glyceraldehyde 3-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiNAIVPNP.
OrthoDBiPOG093Z05Z6.

Family and domain databases

HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiView protein in InterPro
IPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PTHR10836:SF76. PTHR10836:SF76. 1 hit.
PfamiView protein in Pfam
PF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.

Sequencei

Sequence statusi: Complete.

A3CYG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVAINGYG TIGKRVADAV AAQPDMEVIG VSKTSVSAEA YIAKERGYPL
60 70 80 90 100
YIADLGKKPA FEKAGIEVAG DVEAMLKAAD IVVDATPGGV GEKNRPIYEK
110 120 130 140 150
LGKKAIFQGG EDHEVAGFSF NAHANYNEAE NHQFARVVSC NSTGLVRIIH
160 170 180 190 200
ALDQAFGVAR VRAVMVRRGA DPDDVKRGPI DAIVLNPASI PSHHGPDVNT
210 220 230 240 250
VLPHINIVTL AMIVPTTFMH MHSIQMDLKK ETTREEVLKV FENHSRIGLV
260 270 280 290 300
RKTMGIKSNA QLREYTQDLG RPRTDLWENG VFEESVSILD GKEFYCFQAI
310 320 330 340
HQEADVVPEN IDCIRALMGT VKDPQESIRM TNEALGLVAI G
Length:341
Mass (Da):37,212
Last modified:March 20, 2007 - v1
Checksum:iFBFCE0F69EA81953
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN58418.1.
RefSeqiWP_011845327.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN58418; ABN58418; Memar_2497.
GeneIDi4848447.
KEGGimem:Memar_2497.

Similar proteinsi

Entry informationi

Entry nameiG3P_METMJ
AccessioniPrimary (citable) accession number: A3CYG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 20, 2007
Last modified: November 22, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families