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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADUniRule annotation1
Binding sitei63NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei127NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi156Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi171IronUniRule annotation1
Binding sitei219NAD; via amide nitrogenUniRule annotation1
Binding sitei229GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 56NADUniRule annotation2
Nucleotide bindingi154 – 156NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Memar_2251
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003007381 – 254Thiamine thiazole synthaseAdd BLAST254

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_2251.

Structurei

3D structure databases

ProteinModelPortaliA3CXS4.
SMRiA3CXS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG093Z09PU.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

A3CXS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNEVTISR AILEEQHRAL IDHLEMDAAV IGGGPSGLAC AALLGEKGVK
60 70 80 90 100
CALIEKKLSI GGGMWGGGMM FPRIVVQEDA RRLLDRFGIA YKAFEEGYYV
110 120 130 140 150
AKSVEAVAKL TAAACDAGVE FFNLTTVEDV MIRGDGRIGG LVVNWTPVDM
160 170 180 190 200
AGLHVDPLTM ACTCTVDATG HDAMIARMVE KKGGALTVKG ESFMWAERAE
210 220 230 240 250
SQILAHTKEV FPGLFVTGMA ANAVAGECRM GPIFGGMLLS GERAAELVAE

RLGR
Length:254
Mass (Da):26,950
Last modified:March 20, 2007 - v1
Checksum:i70DDE680D7D782B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN58174.1.
RefSeqiWP_011845083.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN58174; ABN58174; Memar_2251.
GeneIDi4846229.
KEGGimem:Memar_2251.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN58174.1.
RefSeqiWP_011845083.1. NC_009051.1.

3D structure databases

ProteinModelPortaliA3CXS4.
SMRiA3CXS4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368407.Memar_2251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN58174; ABN58174; Memar_2251.
GeneIDi4846229.
KEGGimem:Memar_2251.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG093Z09PU.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_METMJ
AccessioniPrimary (citable) accession number: A3CXS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 20, 2007
Last modified: May 10, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.