A3CXE7 (DP2L_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Cleaved into the following chain:

1. Memar polC intein
   Alternative name(s):
   Memar pol II intein

Gene names

Name:	polC
Ordered Locus Names:	Memar_2124

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	1285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 870	870	DNA polymerase II large subunit, 1st part Potential		PRO_0000294688
	Chain	871 – 1035	165	Memar polC intein Potential		PRO_0000294689
	Chain	1036 – 1285	250	DNA polymerase II large subunit, 2nd part Potential		PRO_0000294690

Sequences

	Sequence		Length	Mass (Da)
	A3CXE7 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 7DF1F55811763A22	FASTA	1,285	142,303
	10 20 30 40 50 60 MEVSPAIARY FEELEGELDA AIRLAAAARA RGLDPRTEIE IPVASDLADR VEALLGYKGI 70 80 90 100 110 120 AARIRELEAE MSREEAALRI GDDFAARKFG ETTPEEILDH AIRAAMALLT EGVVAAPTEG 130 140 150 160 170 180 IGKVSLGKND DGTDYLKIYY AGPIRSAGGT AQALSVLVGD YVRQALGINR YIPRPEEVER 190 200 210 220 230 240 YIEEIRQYNN IMSLQYLPSE KELRMIIENC PVCIDGEPTE QQEVSGYRNL ERVETNTVRG 250 260 270 280 290 300 GMALVVAEGL ALKAPKVLKN VRKMKMEGWD WIEEMIGGGP KSDDDDASAA IKPKDKYIRD 310 320 330 340 350 360 LIGGRPVFSY PMRKGGFRLR LGRARNTGFA AAGFNPATLH ILGDFLAVGT QMKVERPGKA 370 380 390 400 410 420 AGVVPVDSIQ GPTVKLRSGE VRRVDDAAEA RRLAGQVDEI LDVGEMLVSF GEFMENNHPL 430 440 450 460 470 480 MPPAYCEEWW MLEGGPRHPE NELEAIEFAL DGVPLHPDYT YMWDDVAPAD IARLAEAVGT 490 500 510 520 530 540 GGTVEDGVLM IRNTPETKAI LEELLIPHHL SGDRLAIREH LAFLACLGLT LQLTKRPAWQ 550 560 570 580 590 600 DAPMENSLDL VMHLSGFTVR SRAGTRIGGR MGRPGKSKPR EMRPPPHSLF PIGDEGGARR 610 620 630 640 650 660 SFQAACSSKP RSNTDGGVIE AEVGERQCPA CGAFTYKNLC ECGAHTNPVF RCPRCGKDVG 670 680 690 700 710 720 QDVCPRCNAE TVCLQKVTIN VKAEYLAAME SLGVRESSVA LLKGVKGLIS RERPVEPIEK 730 740 750 760 770 780 GILRALQNLY VFKDGTVRYD MIDLPLTHFR PDEVGVPIER LRELGYTHDT YGRELVSDDQ 790 800 810 820 830 840 VLELRHQDIL VSEGCGEWLV RVAKFVDDLL VRLYGLEPFY KAEKPLDLVG HLLMGLAPHT 850 860 870 880 890 900 SAGVLVRLIG FSKAPVGYGH PFFHAAKRRN CFAGDTEITV SDGRRWMSLP IRQFVTENFD 910 920 930 940 950 960 ISKPGLDHVG TFYSDPRQPF YVRSIDSQGK TSLKRVTSVS VHRAPAHLVR FATRRGRVLT 970 980 990 1000 1010 1020 VTPDHAMLVW DTDYLRKIKA LEVAVGDRVP VEEGGLVVAD EVVSRETVQA LDDRVYCLTV 1030 1040 1050 1060 1070 1080 AENHTLVANG IFCGQCDGDE DCVMLLLDGL INFSRAYLPE TRGGTMDAPL VLTTRIDPSE 1090 1100 1110 1120 1130 1140 VDKESHNVDV CDHYPIEVYN GCLAYAHPKD LDAFVDRVER RLGTPAQCEG FLFTHQTSNI 1150 1160 1170 1180 1190 1200 SAGPLESTYT RLGSMLDKLE AELDLAKRIR AVDEDDVAER VLNTHFIRDL QGNLNAFSKQ 1210 1220 1230 1240 1250 1260 KVRCMKCNAK YRRMPLAGKC TRCGGHVIPT VHEGSVKKYL EMSRNICATY AISDYTKQRV 1270 1280 EVLFMQIEST FGEPPEKQLG LADFM

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed: 21304656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN58047.1.
RefSeq	YP_001048029.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CXE7.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CXE7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4847324.
GenomeReviews	Gene locus Memar_2124 in contig CP000562_GR.
KEGG	mem:Memar_2124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK14714.
Enzyme and pathway databases
BioCyc	MMAR368407:MEMAR_2124-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006141. Intein_splice_site. IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. IPR019775. WD40_repeat_CS. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01445. Intein_Nterm. 1 hit. TIGR00354. PolC. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DP2L_METMJ

Accession

Primary (citable) accession number: A3CXE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 20, 2007
Last modified:	November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program