A3CX68 (SYP_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Memar_2042

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Proline--tRNA ligase HAMAP MF_01571		PRO_0000288421

Sequences

	Sequence		Length	Mass (Da)
	A3CX68 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 7E6C1AF9AA7B9E9C	FASTA	477	53,180
	10 20 30 40 50 60 MEEDTGALPR KENFSEWYND ILWRAEIMDV RYPVKGLYVW YPHGFGIRKR AYGVLRDLMD 70 80 90 100 110 120 RDHAETMFPL LIPKTEFMKE AEHIKGFEDE VYWVTHGGRN ELDVPLALRP TSETAIYPMY 130 140 150 160 170 180 ALWIRSHTDL PLRLYQVVNT FRYETKHTRP LIRLREITSF KEAHTVHATR EEAAAQVETA 190 200 210 220 230 240 IALYREFYDT LRVPVILSRR PAWDKFPGAD YTIAADTIMP DGKTLQIGTV HMLGDHFSRT 250 260 270 280 290 300 YAITYEDANG EQQYAYQTCY GISERSIAAV VSVHGDDKGL VLPPEVAPLE VVIVPIIVGK 310 320 330 340 350 360 RRDEVLASAA ALEAELRGAG FAVKLDARDM RPGAKYYHWE MRGVPLRVEV GPRDIDANTV 370 380 390 400 410 420 VAVARTGKKT TLDRRGVVEG ITSVLAEFGE DLSQAARQAM AARITAAETL EETAEAVKSG 430 440 450 460 470 VAVVHWCGSQ ECAEKIEAAV DASIIGSDIR SDLIAVSDGP CVACGGEGTS ALVARTY

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed: 21304656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN57968.1.
RefSeq	YP_001047950.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CX68.
SMR	A3CX68. Positions 13-477.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CX68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4847873.
GenomeReviews	Gene locus Memar_2042 in contig CP000562_GR.
KEGG	mem:Memar_2042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04466.
HOGENOM	HBG334108.
OMA	KFAEYEL.
PhylomeDB	A3CX68.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MMAR368407:MEMAR_2042-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METMJ

Accession

Primary (citable) accession number: A3CX68

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 20, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program