A3CWW3 (NIFH_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogenase iron protein
EC=1.18.6.1

Alternative name(s):
Nitrogenase Fe protein
Nitrogenase component II
Nitrogenase reductase

Gene names

Name:	nifH
Ordered Locus Names:	Memar_1937

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus › Methanoculleus marisnigri

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein By similarity. HAMAP-Rule MF_00533
Catalytic activity	8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate. HAMAP-Rule MF_00533
Cofactor	Binds 1 4Fe-4S cluster per dimer By similarity.
Subunit structure	Homodimer By similarity.
Post-translational modification	The reversible ADP-ribosylation of Arg-98 inactivates the nitrogenase reductase and regulates nitrogenase activity By similarity. HAMAP-Rule MF_00533
Sequence similarities	Belongs to the NifH/BchL/ChlL family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	ADP-ribosylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Cellular_component	molybdenum-iron nitrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: HAMAP carbonyl sulfide nitrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Nitrogenase iron protein HAMAP-Rule MF_00533		PRO_1000211875
Regions
	Nucleotide binding	8 – 15	8	ATP Potential
Sites
	Metal binding	95	1	Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	128	1	Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Amino acid modifications
	Modified residue	98	1	ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3CWW3 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 412E1422A02E1AD9	FASTA	272	29,646
	10 20 30 40 50 60 MKQIALYGKG GIGKSTTAAN LSAALAGEGL DILQIGCDPK HDSTRMLMHG TWIPTVLDLI 70 80 90 100 110 120 RERGDEKITV DDVVYRGFRG VRCVEAGGPE PGIGCAGRGI IATFQLLERL NALKGDVIVY 130 140 150 160 170 180 DVLGDVVCGG FAMPMREGYA QEIYLVTSGE LMSIYAANNI AKAIARLSRR VRSRCTLGGV 190 200 210 220 230 240 ICNAKNIEGE RELVDEFARR IGSRLIAYIP RSRIVQVAEL QKQTVVEYAP ESDQAAVYQE 250 260 270 LARTVYGNET TSIPTPLEMD ELESFALEFV QV

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN57863.1.
RefSeq	YP_001047845.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CWW3.
ModBase	Search...
Protein-protein interaction databases
STRING	368407.Memar_1937.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN57863; ABN57863; Memar_1937.
GeneID	4847172.
KEGG	mem:Memar_1937.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1348.
HOGENOM	HOG000228825.
KO	K02588.
OMA	HDSTRML.
ProtClustDB	CLSK949511.
Enzyme and pathway databases
BioCyc	MMAR368407:GH7L-1990-MONOMER.
Family and domain databases
HAMAP	MF_00533. NifH.
InterPro	IPR000392. Nitogenase_NifH/Reductase_ChlL. IPR005977. Nitrogenase_Fe_NifH. [Graphical view]
Pfam	PF00142. Fer4_NifH. 1 hit. [Graphical view]
PIRSF	PIRSF000363. Nitrogenase_iron. 1 hit.
PRINTS	PR00091. NITROGNASEII.
TIGRFAMs	TIGR01287. nifH. 1 hit.
PROSITE	PS00746. NIFH_FRXC_1. 1 hit. PS00692. NIFH_FRXC_2. 1 hit. PS51026. NIFH_FRXC_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIFH_METMJ

Accession

Primary (citable) accession number: A3CWW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	March 20, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program