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Protein

Flap endonuclease 1

Gene

fen

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity).By similarity

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Magnesium 1UniRule annotation1
Metal bindingi81Magnesium 1UniRule annotation1
Metal bindingi153Magnesium 1UniRule annotation1
Metal bindingi155Magnesium 1UniRule annotation1
Metal bindingi174Magnesium 2UniRule annotation1
Metal bindingi176Magnesium 2UniRule annotation1
Metal bindingi235Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMMAR368407:G1G85-1933-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:fenUniRule annotation
Ordered Locus Names:Memar_1926
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000613261 – 333Flap endonuclease 1Add BLAST333

Interactioni

Subunit structurei

Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_1926

Structurei

3D structure databases

ProteinModelPortaliA3CWV2
SMRiA3CWV2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 99N-domainAdd BLAST99
Regioni117 – 255I-domainAdd BLAST139
Regioni325 – 333Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04050 Archaea
COG0258 LUCA
HOGENOMiHOG000193852
KOiK04799
OMAiGSQDYDS
OrthoDBiPOG093Z0642

Family and domain databases

CDDicd09867 PIN_FEN1, 1 hit
HAMAPiMF_00614 Fen, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR023426 Flap_endonuc
IPR019973 Flap_structure-sp_endonuc_arc
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR03674 fen_arch, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit

Sequencei

Sequence statusi: Complete.

A3CWV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVAIRDILA DCKETLTWDD LSGIAALDAH NALYQFLSII RQPDGTPLMN
60 70 80 90 100
GAGRITSHLS GILFRTVNFL EKGIRPVFVF DGKPPEFKQE TINERREHRA
110 120 130 140 150
RADEAWKTAL REGDMEEAYK QASASARIDS HTIASSRELL DLLGIPWVQA
160 170 180 190 200
PSEGEAQAAY MARQGKVTYA VSQDYDSLLF GSPVLVRNLT VSGRRKTRGR
210 220 230 240 250
TITVNPERIV LSSFLDRLGV TREQLVKIGI LVGTDFNPGI RGVGGKTALK
260 270 280 290 300
IVRNGEFESV IAEKQPDFNP APIRDFFLNP PVTDDYTLEW RTPDVEGVVE
310 320 330
MLCGRYDFSE ERVRSALAKV SVKATQKTLD AWF
Length:333
Mass (Da):37,100
Last modified:March 20, 2007 - v1
Checksum:i92928D5B4B34BC0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA Translation: ABN57852.1
RefSeqiWP_011844761.1, NC_009051.1

Genome annotation databases

EnsemblBacteriaiABN57852; ABN57852; Memar_1926
GeneIDi4846283
KEGGimem:Memar_1926

Similar proteinsi

Entry informationi

Entry nameiFEN_METMJ
AccessioniPrimary (citable) accession number: A3CWV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health