Reviewed, UniProtKB/Swiss-Prot A3CWS6 (GUAAB_METMJ)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Memar_1900

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_1000048379
Regions
	Domain	33 – 185	153	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3CWS6-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 3C7E7255A2FD49A7	FASTA	305	33,177
	10 20 30 40 50 60 MVNIEKFIDQ AVREIRDAAG EDKVVMALSG GVDSSVCAAL ATRAIGDQLV PIYVDTGLMR 70 80 90 100 110 120 KGETDRIRSL FADANLRVVD AADEFFEALA GIVDPEEKRK AIGAKFIRIF EREAKRTGAT 130 140 150 160 170 180 MLLQGTIYPD RIESEGGIKS HHNVGGMPLD IEFKGVIEPL ADLYKDEVRE VAGGLGLPAE 190 200 210 220 230 240 IQHRMPFPGP GLAVRVLGEV TKEKIAIVRE ANAIVEECLV EEFRPWQCFA ALIGLGTGVK 250 260 270 280 290 300 GDVRLHGWIV AVRAVGSRDG MTADPLLLPY ETLSRMATRI TAEIPGVARV VYDVTPKPPA TIEYE

References

[1]

"Complete sequence of Methanoculleus marisnigri JR1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Anderson I., Sieprawska-Lupa M., Whitman W., Woese C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000562 Genomic DNA. Translation: ABN57826.1.
RefSeq	YP_001047808.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A3CWS6.
Genome annotation databases
GeneID	4848091.
GenomeReviews	Gene locus Memar_1900 in contig CP000562_GR.
KEGG	mem:Memar_1900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GIKSHHN.
Family and domain databases
HAMAP	MF_00345. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. IPR018318. tRNA_MeTrfase-like. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00958. GMP_synt_C. 1 hit. PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_METMJ

Accession

Primary (citable) accession number: A3CWS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 20, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)