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A3CWP1 (DNLI_METMJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]
Gene names
Name:lig
Ordered Locus Names:Memar_1865
OrganismMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]
Taxonomic identifier368407 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548DNA ligase HAMAP-Rule MF_00407
PRO_0000365255

Sites

Active site2461N6-AMP-lysine intermediate By similarity
Binding site2441ATP By similarity
Binding site2511ATP By similarity
Binding site2661ATP By similarity
Binding site2951ATP By similarity
Binding site3341ATP By similarity
Binding site4051ATP By similarity
Binding site4111ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A3CWP1 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 3544373E6F328D03

FASTA54861,157
        10         20         30         40         50         60 
MQFLEFAQVC EHLEGTPGRL DMIEQVAAVL PRLDDEELPV FVRFVMGRVF PDWSTKKLGV 

        70         80         90        100        110        120 
GPNLLYDAVA YVVGTKRETV REAINTTGDV GLAVEGLLAR KEQTSFFIQE LDLLDVYREL 

       130        140        150        160        170        180 
ERMAAAEGQR SQREKLRVAQ GLFGNARPLE GRYLARLLLE ELRIGMGEGN VRDAVAKAFE 

       190        200        210        220        230        240 
LDVRLVEHAH QALNDLGEVA LLARRDPDAL SGVTIEPFRP VKMMLAQAGT IAAQIEDHGE 

       250        260        270        280        290        300 
VAVEYKYDGS RFQFHKVGDV CRIYSRRLED VTESLPDIAN LLLEATDHDV ILDGEAVAVR 

       310        320        330        340        350        360 
DGKPMPFQYV IRRFRRKHEV DSMMEKIELV PMVFDILYLD GETLMDRPLA ERRKALDEVL 

       370        380        390        400        410        420 
GAHVAPQFPA TDAAAAEAIY AEALDLGHEG VMVKVLDSPY TPGVRGRLWV KVKPGVETLD 

       430        440        450        460        470        480 
LVVVGAEWGE GRRAGTFGSF LLAVQDQGRL LPVGKVATGI TDEVLAELYA LFKDRVIARS 

       490        500        510        520        530        540 
GKEVTLEPEV VFEVGYSEIQ TSPNYESGYA LRFPRFVRVR EDKSVDETET LDSLAERYGR 


QRNGQGSL 

« Hide

References

[1]"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1."
Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. expand/collapse author list , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000562 Genomic DNA. Translation: ABN57791.1.
RefSeqYP_001047773.1. NC_009051.1.

3D structure databases

ProteinModelPortalA3CWP1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING368407.Memar_1865.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN57791; ABN57791; Memar_1865.
GeneID4846179.
KEGGmem:Memar_1865.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAARVQVHK.

Enzyme and pathway databases

BioCycMMAR368407:GH7L-1910-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI_METMJ
AccessionPrimary (citable) accession number: A3CWP1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 20, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families