Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA ligase

Gene

lig

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei244 – 2441ATPUniRule annotation
Active sitei246 – 2461N6-AMP-lysine intermediateUniRule annotation
Binding sitei251 – 2511ATPUniRule annotation
Binding sitei266 – 2661ATPUniRule annotation
Binding sitei295 – 2951ATPUniRule annotation
Binding sitei334 – 3341ATPUniRule annotation
Binding sitei405 – 4051ATPUniRule annotation
Binding sitei411 – 4111ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR368407:GH7L-1910-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Memar_1865
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000002146 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548DNA ligasePRO_0000365255Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi368407.Memar_1865.

Structurei

3D structure databases

ProteinModelPortaliA3CWP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiGRPRPFQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CWP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFLEFAQVC EHLEGTPGRL DMIEQVAAVL PRLDDEELPV FVRFVMGRVF
60 70 80 90 100
PDWSTKKLGV GPNLLYDAVA YVVGTKRETV REAINTTGDV GLAVEGLLAR
110 120 130 140 150
KEQTSFFIQE LDLLDVYREL ERMAAAEGQR SQREKLRVAQ GLFGNARPLE
160 170 180 190 200
GRYLARLLLE ELRIGMGEGN VRDAVAKAFE LDVRLVEHAH QALNDLGEVA
210 220 230 240 250
LLARRDPDAL SGVTIEPFRP VKMMLAQAGT IAAQIEDHGE VAVEYKYDGS
260 270 280 290 300
RFQFHKVGDV CRIYSRRLED VTESLPDIAN LLLEATDHDV ILDGEAVAVR
310 320 330 340 350
DGKPMPFQYV IRRFRRKHEV DSMMEKIELV PMVFDILYLD GETLMDRPLA
360 370 380 390 400
ERRKALDEVL GAHVAPQFPA TDAAAAEAIY AEALDLGHEG VMVKVLDSPY
410 420 430 440 450
TPGVRGRLWV KVKPGVETLD LVVVGAEWGE GRRAGTFGSF LLAVQDQGRL
460 470 480 490 500
LPVGKVATGI TDEVLAELYA LFKDRVIARS GKEVTLEPEV VFEVGYSEIQ
510 520 530 540
TSPNYESGYA LRFPRFVRVR EDKSVDETET LDSLAERYGR QRNGQGSL
Length:548
Mass (Da):61,157
Last modified:March 20, 2007 - v1
Checksum:i3544373E6F328D03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN57791.1.
RefSeqiWP_011844700.1. NC_009051.1.
YP_001047773.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN57791; ABN57791; Memar_1865.
GeneIDi4846179.
KEGGimem:Memar_1865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN57791.1.
RefSeqiWP_011844700.1. NC_009051.1.
YP_001047773.1. NC_009051.1.

3D structure databases

ProteinModelPortaliA3CWP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368407.Memar_1865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN57791; ABN57791; Memar_1865.
GeneIDi4846179.
KEGGimem:Memar_1865.

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK10747.
OMAiGRPRPFQ.

Enzyme and pathway databases

BioCyciMMAR368407:GH7L-1910-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35101 / DSM 1498 / JR1.

Entry informationi

Entry nameiDNLI_METMJ
AccessioniPrimary (citable) accession number: A3CWP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 20, 2007
Last modified: April 29, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.