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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR368407:G1G85-1817-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Memar_1817
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003310091 – 561Glutamate--tRNA ligaseAdd BLAST561

Interactioni

Protein-protein interaction databases

STRINGi368407.Memar_1817

Structurei

3D structure databases

ProteinModelPortaliA3CWJ3
SMRiA3CWJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi107 – 117"HIGH" regionUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04302 Archaea
COG0008 LUCA
HOGENOMiHOG000073585
KOiK01885
OMAiMRFAPNP
OrthoDBiPOG093Z013J

Family and domain databases

Gene3Di2.40.240.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A3CWJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGDIEHLLF IYALQNAVKH DSAPKSGTVI GTVLGKHPEF RSRARELGPL
60 70 80 90 100
AGKAVAQVGK MSAADRKSRL EALAPELLAE LTETHEHVRK LPDLEGAENG
110 120 130 140 150
VVMRFAPNPS GPLHLGHARA SILNDYYVRR YGGRYVLRVE DTDPKRVDPD
160 170 180 190 200
AYAMVLEDVE WLGLGITDIV YQSDRLDIYY DWCRKLIELG GAYVCVCDSE
210 220 230 240 250
RFRELKLKGK ACPCRELTVE ENLELWQQML DGEFYEGDVT VRVKTDLAHP
260 270 280 290 300
DPAMRDYSAM RIVNAPLHPR VDATVFPLMN FSVAVDDHLL GITHVIRGKD
310 320 330 340 350
HIANTRRQRY IFDYFGWKPP VYRHYGRMGI SGVVLSTSGM REGINSGLYT
360 370 380 390 400
GWDDIHLGTM RAIARRGIEP EAVRNAMVDI GIGETDISFS WENLYSRNKE
410 420 430 440 450
LVDPKANRYF FVPDPVEVTV EGAPRREAHA ALHPNDPSRG VRTLVAEEAI
460 470 480 490 500
LLPRADIEGK SMVRLKDLYN VKIAWDGDTP RVSYAGDSLE EARHEKAPII
510 520 530 540 550
QWLPADAKLP CTLLRQDGSL EGFCEPLVAG ETDRVVQFER IGFARIDSAD
560
GGRVSAYFAH R
Length:561
Mass (Da):62,974
Last modified:March 20, 2007 - v1
Checksum:i824484630EBCA2A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA Translation: ABN57743.1
RefSeqiWP_011844652.1, NC_009051.1

Genome annotation databases

EnsemblBacteriaiABN57743; ABN57743; Memar_1817
GeneIDi4846059
KEGGimem:Memar_1817

Similar proteinsi

Entry informationi

Entry nameiSYE_METMJ
AccessioniPrimary (citable) accession number: A3CWJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health