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A3CWJ3 (SYE_METMJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Memar_1817
OrganismMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]
Taxonomic identifier368407 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus

Protein attributes

Sequence length561 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 561561Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000331009

Regions

Motif107 – 11711"HIGH" region HAMAP-Rule MF_00022

Sequences

Sequence LengthMass (Da)Tools
A3CWJ3 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 824484630EBCA2A5

FASTA56162,974
        10         20         30         40         50         60 
MDGDIEHLLF IYALQNAVKH DSAPKSGTVI GTVLGKHPEF RSRARELGPL AGKAVAQVGK 

        70         80         90        100        110        120 
MSAADRKSRL EALAPELLAE LTETHEHVRK LPDLEGAENG VVMRFAPNPS GPLHLGHARA 

       130        140        150        160        170        180 
SILNDYYVRR YGGRYVLRVE DTDPKRVDPD AYAMVLEDVE WLGLGITDIV YQSDRLDIYY 

       190        200        210        220        230        240 
DWCRKLIELG GAYVCVCDSE RFRELKLKGK ACPCRELTVE ENLELWQQML DGEFYEGDVT 

       250        260        270        280        290        300 
VRVKTDLAHP DPAMRDYSAM RIVNAPLHPR VDATVFPLMN FSVAVDDHLL GITHVIRGKD 

       310        320        330        340        350        360 
HIANTRRQRY IFDYFGWKPP VYRHYGRMGI SGVVLSTSGM REGINSGLYT GWDDIHLGTM 

       370        380        390        400        410        420 
RAIARRGIEP EAVRNAMVDI GIGETDISFS WENLYSRNKE LVDPKANRYF FVPDPVEVTV 

       430        440        450        460        470        480 
EGAPRREAHA ALHPNDPSRG VRTLVAEEAI LLPRADIEGK SMVRLKDLYN VKIAWDGDTP 

       490        500        510        520        530        540 
RVSYAGDSLE EARHEKAPII QWLPADAKLP CTLLRQDGSL EGFCEPLVAG ETDRVVQFER 

       550        560 
IGFARIDSAD GGRVSAYFAH R 

« Hide

References

[1]"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1."
Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. expand/collapse author list , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000562 Genomic DNA. Translation: ABN57743.1.
RefSeqYP_001047725.1. NC_009051.1.

3D structure databases

ProteinModelPortalA3CWJ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING368407.Memar_1817.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN57743; ABN57743; Memar_1817.
GeneID4846059.
KEGGmem:Memar_1817.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000073585.
KOK01885.
OMAYLGWDDP.

Enzyme and pathway databases

BioCycMMAR368407:GH7L-1856-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_A. Glu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00463. gltX_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_METMJ
AccessionPrimary (citable) accession number: A3CWJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 20, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries