A3CW66 (PYRB_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	Memar_1689

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Subunit structure	Heterooligomer of catalytic and regulatory chains.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000321187

Sequences

	Sequence		Length	Mass (Da)
	A3CW66 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 6A7E87E39D4AA32B	FASTA	298	33,146
	10 20 30 40 50 60 MYHIISIRDF ERSDLDYLLD RAQEFDTGKY RPGMLDDKLV ALLFFEPSTR TRMSFATAMA 70 80 90 100 110 120 RLGGRSISVD SVEASSIVKG ETLADTIRVV SGYADAIVLR HPKEGAARLA SEFATVPVIN 130 140 150 160 170 180 AGDGAGQHPS QTLLDLYTIR QSMPVDGIDV GLLGDLRYGR TAHSLALALS LYGVTLHTIA 190 200 210 220 230 240 PVGLEMPANI ALELRERGME VVEHPNVEEA IRELDVLYVT RIQRERFPDS ASYYNVASSY 250 260 270 280 290 RITTDLLDGV KERLMILHPL PRAGEIDPAV DRTPYARYFE QARNGVPIRM ALLHEVMK

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed: 21304656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN57616.1.
RefSeq	YP_001047598.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CW66.
SMR	A3CW66. Positions 2-297.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CW66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4847953.
GenomeReviews	Gene locus Memar_1689 in contig CP000562_GR.
KEGG	mem:Memar_1689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04260.
HOGENOM	HBG579429.
OMA	DLYTIRQ.
PhylomeDB	A3CW66.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	MMAR368407:MEMAR_1689-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_METMJ

Accession

Primary (citable) accession number: A3CW66

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 20, 2007
Last modified:	November 16, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program