A3CVP2 (SYA_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Memar_1513

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	915 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 915	915	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_1000074512
Sites
	Metal binding	609	1	Zinc By similarity
	Metal binding	613	1	Zinc By similarity
	Metal binding	712	1	Zinc By similarity
	Metal binding	716	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3CVP2 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: B9F5C81F183BA4FE	FASTA	915	101,646
	10 20 30 40 50 60 MLEEEYTLDY FRSEGFERKV CKSCGAAFWT RDPEQEFCGD APCVTYNFIG NPVFKPHNVS 70 80 90 100 110 120 EMREAFLSFF ERHGHTRLER YPVAARWRDD IYLTIASIAD FQPFVTSGVV PPPANPLTIS 130 140 150 160 170 180 QPCIRLNDLD SVGRSGRHLT LFEMMAHHAF NTPEEQIYWK DQTVALCDEF IKSIGGDPAR 190 200 210 220 230 240 VSYKEHPWYG GGNAGASVEV LIGGLEVATL VFMNLGRQKT DQPPVDVNGV PYYPMRLNIV 250 260 270 280 290 300 DTGYGLERLV WASKGSPTIY DAVFPEMVSR LMRSARLEDL LDNPEFTKIM GLSARFAGVM 310 320 330 340 350 360 DISGTNLYNL RRKVAEAIDV PVERLERIVV PIEKVYSIAD HTRCLAYMLG DCIVPSNVRE 370 380 390 400 410 420 GYLARLVLRR TLRMMNDLSM DDALTDLIEA QMQVVGAENF EQDVDAVREI VENEEARYAS 430 440 450 460 470 480 TLERGARIVQ KIARNYRAKS SRVPLEEVIT LYDSHGIPPE MVKEVAAAEG AVVEIPDNFY 490 500 510 520 530 540 SLIAETHSEA QKEARGEDPL DAYRERAVSL PPTKKLYYEL PNEVEFEAMV LDYFDGMAVL 550 560 570 580 590 600 DQTLFYPEGG GQPSDTGTLV TSESMVRVEE VVKLGEVILH RVTGGPLMRG DRVKGMVDEE 610 620 630 640 650 660 RRWSLMRHHT ATHVLLHAAQ QVLGVHVHQA GAQKGSEVSR LDIRHYRHIT PDELRRIELE 670 680 690 700 710 720 ANRLVMADTP VYIHVEERTK AEQKYGFGLY QGGVPPGREI RTVQVGADVQ ACAGTHVRTT 730 740 750 760 770 780 GEIGPIRVLG VEHIQDGVER LVFAAGIAAV HAVQHLGDLL QESADMVSVQ PENLPATVAR 790 800 810 820 830 840 FFSEWKEQKK EIERLQKKVV DLEMQNLDGE VVDGVRVVVR TLDATHKELV ALATTVADEG 850 860 870 880 890 900 GVALFASSDG TVKVVATSGA PTVNAVDIVR EVCGILGGKG GGKPNLAQGA GPDASRLEEA 910 LEYGRNRIIE ALHGE

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed: 21304656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN57442.1.
RefSeq	YP_001047424.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CVP2.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CVP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4846756.
GenomeReviews	Gene locus Memar_1513 in contig CP000562_GR.
KEGG	mem:Memar_1513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04130.
HOGENOM	HBG392147.
OMA	EIDIFRT.
PhylomeDB	A3CVP2.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MMAR368407:MEMAR_1513-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METMJ

Accession

Primary (citable) accession number: A3CVP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 20, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program