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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation
Metal bindingi178 – 1781Magnesium; via carbamate groupUniRule annotation
Metal bindingi180 – 1801MagnesiumUniRule annotation
Metal bindingi181 – 1811MagnesiumUniRule annotation
Active sitei270 – 2701Proton acceptorUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Binding sitei303 – 3031SubstrateUniRule annotation
Sitei310 – 3101Transition state stabilizerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciMMAR368407:GH7L-1351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Memar_1325Imported
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)Imported
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000002146 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_1325.

Structurei

3D structure databases

ProteinModelPortaliA3CV55.
SMRiA3CV55. Positions 3-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni353 – 3553Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230831.
KOiK01601.
OMAiHAAFTRN.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CV55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDWYEEFV DLNHTPGPDE LVALYYFEPA AGISKEEAVG RIASESSTGT
60 70 80 90 100
WTTLFTLPPR MRDLQAKAFE IEGNYVKIAY PLALWEEGNA AQLLSGIAGN
110 120 130 140 150
VFGMKALDRL RLIDASLPAE YLRHFKGPHF GMEGIRDMMK IRGRPLTGAV
160 170 180 190 200
PKPKVGFTAE EHAEVGYETW MGGFDFVKDD ENLTSQSFNR FDDRVRAMAK
210 220 230 240 250
MRDRAEQETG DVKSAFINIT ADTETMEKRA KMLADYGWNY AMIDVVVAGT
260 270 280 290 300
AAVATLRDYC SDLGLAIHAH RAMHAAFDRD EKHGITMQFL AKMMRLVGVA
310 320 330 340 350
QIHTGTAVGK LVGTRAEATV LADMLREKHT NAVDHMALDQ DWGNIKSAFP
360 370 380 390 400
VSSGGLHPGL VPDVLDIYGS ELVLLVSGGI HGHPKGTRAG AEAAMQAIEA
410 420 430
WKEGETLEEK AKKAPALSEA LEKWGRYKPK
Length:430
Mass (Da):47,331
Last modified:March 20, 2007 - v1
Checksum:i08D87E9C4B244477
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN57255.1.
RefSeqiWP_011844166.1. NC_009051.1.
YP_001047237.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN57255; ABN57255; Memar_1325.
GeneIDi4848035.
KEGGimem:Memar_1325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN57255.1.
RefSeqiWP_011844166.1. NC_009051.1.
YP_001047237.1. NC_009051.1.

3D structure databases

ProteinModelPortaliA3CV55.
SMRiA3CV55. Positions 3-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368407.Memar_1325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN57255; ABN57255; Memar_1325.
GeneIDi4848035.
KEGGimem:Memar_1325.

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230831.
KOiK01601.
OMAiHAAFTRN.

Enzyme and pathway databases

BioCyciMMAR368407:GH7L-1351-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35101 / DSM 1498 / JR1Imported.

Entry informationi

Entry nameiA3CV55_METMJ
AccessioniPrimary (citable) accession number: A3CV55
Entry historyi
Integrated into UniProtKB/TrEMBL: March 20, 2007
Last sequence update: March 20, 2007
Last modified: May 27, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.