A3CUF2 (CIMA_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative (R)-citramalate synthase CimA
EC=2.3.1.182

Gene names

Name:	cimA
Ordered Locus Names:	Memar_1069

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus › Methanoculleus marisnigri

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate By similarity. HAMAP-Rule MF_01028
Catalytic activity	Acetyl-CoA + pyruvate + H2O = CoA + (2R)-2-hydroxy-2-methylbutanedioate. HAMAP-Rule MF_01028
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3. HAMAP-Rule MF_01028
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP leucine biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	(R)-citramalate synthase activity Inferred from electronic annotation. Source: InterPro 2-isopropylmalate synthase activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Putative (R)-citramalate synthase CimA HAMAP-Rule MF_01028		PRO_0000407913

Sequences

	Sequence		Length	Mass (Da)
	A3CUF2 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: EA9E955BCE0B7118	FASTA	503	53,275
	10 20 30 40 50 60 MIVLFVEPIR FFDTTLRDGE QTPGVSLTPA GKLEIATHLA DVGVHVIEAG SAAASVGERE 70 80 90 100 110 120 SIRAIADAGL AAECCTYVRA LPGDIDLAAD AGADSVHLVV PVSDLHIAKK LRKTREQVSE 130 140 150 160 170 180 MAWSAVEYAK ERGLVVELSG EDASRADQDF LAEVFREGVE RGADRLCFCD TVGLLTPERA 190 200 210 220 230 240 AAIIPPLLFA PLSIHCHDDL GFGLATTVAA LRAGATCAHV TVNGLGERAG NTSLEELVMA 250 260 270 280 290 300 LEVLYGVDTG IATEELYPLS THVARLTGVP LATNKPIVGE MAFTHESGIH AHGVMRDAST 310 320 330 340 350 360 YEPLQPERVG RRRRIVLGKH SGSAAVEAAL HDMGYAPSAA QLKEIVDRIK RLGDAGMRIT 370 380 390 400 410 420 DADIMAIADT VMEIEFTPCI ELRQFTIVSG SNAIPTASVT MLVRGEEITG AAVGTGPVDA 430 440 450 460 470 480 AIRALQRSVA DVGSVRLDEY SVDAITGGTD ALVDVSVKLS KDGKTVTSRG ARTDIIMASV 490 500 EAVIAGMNRL LREEHEDRSQ DSD

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN57002.1.
RefSeq	YP_001046984.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CUF2.
ModBase	Search...
Protein-protein interaction databases
STRING	368407.Memar_1069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN57002; ABN57002; Memar_1069.
GeneID	4847429.
KEGG	mem:Memar_1069.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
HOGENOM	HOG000046859.
KO	K09011.
OMA	GARTDII.
ProtClustDB	CLSK949635.
Enzyme and pathway databases
BioCyc	MMAR368407:GH7L-1122-MONOMER.
UniPathway	UPA00047; UER00066.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01028. CimA.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR024890. Citramalate_synthase_CimA. IPR011830. LEU1_arch. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR02090. LEU1_arch. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CIMA_METMJ

Accession

Primary (citable) accession number: A3CUF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	March 20, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program