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Protein

Putative (R)-citramalate synthase CimA

Gene

cimA

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate.UniRule annotation

Catalytic activityi

Acetyl-CoA + pyruvate + H2O = CoA + (2R)-2-hydroxy-2-methylbutanedioate.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-oxobutanoate from pyruvate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative (R)-citramalate synthase CimA (cimA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-oxobutanoate from pyruvate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00047; UER00066.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative (R)-citramalate synthase CimAUniRule annotation (EC:2.3.1.182UniRule annotation)
Gene namesi
Name:cimAUniRule annotation
Ordered Locus Names:Memar_1069
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004079131 – 503Putative (R)-citramalate synthase CimAAdd BLAST503

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_1069.

Structurei

3D structure databases

ProteinModelPortaliA3CUF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 257Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family.UniRule annotation
Contains 1 pyruvate carboxyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG02092. Archaea.
COG0119. LUCA.
HOGENOMiHOG000046859.
KOiK09011.
OMAiGEMFRIV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01028. CimA. 1 hit.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR024890. Citramalate_synthase_CimA.
IPR011830. LEU1_arch.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR02090. LEU1_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CUF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVLFVEPIR FFDTTLRDGE QTPGVSLTPA GKLEIATHLA DVGVHVIEAG
60 70 80 90 100
SAAASVGERE SIRAIADAGL AAECCTYVRA LPGDIDLAAD AGADSVHLVV
110 120 130 140 150
PVSDLHIAKK LRKTREQVSE MAWSAVEYAK ERGLVVELSG EDASRADQDF
160 170 180 190 200
LAEVFREGVE RGADRLCFCD TVGLLTPERA AAIIPPLLFA PLSIHCHDDL
210 220 230 240 250
GFGLATTVAA LRAGATCAHV TVNGLGERAG NTSLEELVMA LEVLYGVDTG
260 270 280 290 300
IATEELYPLS THVARLTGVP LATNKPIVGE MAFTHESGIH AHGVMRDAST
310 320 330 340 350
YEPLQPERVG RRRRIVLGKH SGSAAVEAAL HDMGYAPSAA QLKEIVDRIK
360 370 380 390 400
RLGDAGMRIT DADIMAIADT VMEIEFTPCI ELRQFTIVSG SNAIPTASVT
410 420 430 440 450
MLVRGEEITG AAVGTGPVDA AIRALQRSVA DVGSVRLDEY SVDAITGGTD
460 470 480 490 500
ALVDVSVKLS KDGKTVTSRG ARTDIIMASV EAVIAGMNRL LREEHEDRSQ

DSD
Length:503
Mass (Da):53,275
Last modified:March 20, 2007 - v1
Checksum:iEA9E955BCE0B7118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN57002.1.
RefSeqiWP_011843913.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN57002; ABN57002; Memar_1069.
GeneIDi4847429.
KEGGimem:Memar_1069.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN57002.1.
RefSeqiWP_011843913.1. NC_009051.1.

3D structure databases

ProteinModelPortaliA3CUF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi368407.Memar_1069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN57002; ABN57002; Memar_1069.
GeneIDi4847429.
KEGGimem:Memar_1069.

Phylogenomic databases

eggNOGiarCOG02092. Archaea.
COG0119. LUCA.
HOGENOMiHOG000046859.
KOiK09011.
OMAiGEMFRIV.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00066.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01028. CimA. 1 hit.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR024890. Citramalate_synthase_CimA.
IPR011830. LEU1_arch.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR02090. LEU1_arch. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIMA_METMJ
AccessioniPrimary (citable) accession number: A3CUF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: March 20, 2007
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.