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A3CU83 (PUR2_METMJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Memar_1000
OrganismMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]
Taxonomic identifier368407 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 430430Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018826

Regions

Domain111 – 315205ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Sites

Metal binding2731Magnesium or manganese 1 By similarity
Metal binding2851Magnesium or manganese 1 By similarity
Metal binding2851Magnesium or manganese 2 By similarity
Metal binding2871Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3CU83 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: EF618C6B8DAE8F24

FASTA43046,875
        10         20         30         40         50         60 
MDMKVLVVGG GGREHAITRA LSCNSDTKIF SVMARKNPGI ARLSERVLLE KETNIEKVIP 

        70         80         90        100        110        120 
FATNCGVDAA VIGPEAPLEA GIVDRLEAAG IPSLGPTRAA ARIETDKAFC RRLMERHGIA 

       130        140        150        160        170        180 
GCPEYRVCRD PEEARRFIES YDGDLAVKPI GLTGGKGVRI MGEHVDVAGA VEYAREIGGS 

       190        200        210        220        230        240 
VVLEERLVGE EFTLQAFVDG EHLIPMPLVQ DHKRAYEGDV GPNTGGMGSY SLADHMLPFV 

       250        260        270        280        290        300 
SRRDYEKALR IMTDTVAAMR AEGTPYRGIL YGQFMNTREG PKVIEFNARF GDPEAMNVLS 

       310        320        330        340        350        360 
LLESDFAGIV GHIIEGDLAP SHVRFAKKAT VCKYLVPEGY PDAPRANEPL TLGDYGDALL 

       370        380        390        400        410        420 
YYASVEERDG TLHTLTSRTL AFVGRGETLE EAEAIAEKAA SSVSGSVFHR RDIGTPEILE 

       430 
KRCQHMREIL 

« Hide

References

[1]"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1."
Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. expand/collapse author list , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000562 Genomic DNA. Translation: ABN56933.1.
RefSeqYP_001046915.1. NC_009051.1.

3D structure databases

ProteinModelPortalA3CU83.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING368407.Memar_1000.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN56933; ABN56933; Memar_1000.
GeneID4847274.
KEGGmem:Memar_1000.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycMMAR368407:GH7L-1018-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METMJ
AccessionPrimary (citable) accession number: A3CU83
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways