A3CU64 (A3CU64_METMJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Delta-aminolevulinic acid dehydratase RuleBase RU000515
EC=4.2.1.24 RuleBase RU000515

Gene names

Ordered Locus Names:

Memar_0981

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O. RuleBase RU000515
Subunit structure	Homooctamer By similarity. RuleBase RU000515 PIRSR PIRSR001415-3
Sequence similarities	Belongs to the ALADH family. RuleBase RU004161

Ontologies

Keywords
Biological process	Porphyrin biosynthesis RuleBase RU000515
Ligand	Magnesium PIRSR PIRSR001415-5 Metal-binding PIRSR PIRSR001415-5 Zinc PIRSR PIRSR001415-3
Molecular function	Lyase RuleBase RU000515 EMBL ABN56914.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	197	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Active site	250	1	Schiff-base intermediate with substrate By similarity PIRSR PIRSR001415-1
	Metal binding	118	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	120	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	128	1	Zinc; catalytic By similarity PIRSR PIRSR001415-3
	Metal binding	235	1	Magnesium By similarity PIRSR PIRSR001415-5
	Binding site	207	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	219	1	Substrate 1 By similarity PIRSR PIRSR001415-2
	Binding site	275	1	Substrate 2 By similarity PIRSR PIRSR001415-2
	Binding site	314	1	Substrate 2 By similarity PIRSR PIRSR001415-2

Sequences

	Sequence		Length	Mass (Da)
	A3CU64 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: EC23E00507C561EA	FASTA	327	35,525
	10 20 30 40 50 60 MFPERRMRRM RRRIIQPLLR ETELRKTDLI APVFVDESIS APLPVASMPG QFRHPTDDVA 70 80 90 100 110 120 DYCERLRAAG IRAVLLFGVP AAKDGEATEA YAADGVVQRA VRNIKERLPE IVVITDVCAC 130 140 150 160 170 180 EYTDHGHCGI VGETADGPDL LNDPSLELMA QIAVSHARSG ADIVAPSCML DGMVRAIRQA 190 200 210 220 230 240 LDAAGYQDVL IMSYSSKFAS ALYGPFRDAA DSGFSFGDRT TYQICPGNAR EAVMESELDM 250 260 270 280 290 300 AEGADILMVK PAGAYLDILA AVTGFGLPVA AYQVSGEYAM IKAAAERGWL DERAVAIETL 310 320 TAIKRAGADL IITYFAEDAA RWLDEEQ

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed: 21304656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN56914.1.
RefSeq	YP_001046896.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CU64.
SMR	A3CU64. Positions 4-321.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CU64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4847478.
GenomeReviews	Gene locus Memar_0981 in contig CP000562_GR.
KEGG	mem:Memar_0981.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05029.
HOGENOM	HBG285270.
OMA	ADLCFCE.
PhylomeDB	A3CU64.
ProtClustDB	PRK09283.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001731. Porphobilinogen_synth. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01698.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
SMART	SM01004. ALAD. 1 hit. [Graphical view]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A3CU64_METMJ

Accession

Primary (citable) accession number: A3CU64

Entry history

Integrated into UniProtKB/TrEMBL:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)