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A3CU51 (NADK_METMJ) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:Memar_0968
OrganismMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]
Taxonomic identifier368407 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270NAD kinase HAMAP-Rule MF_00361
PRO_1000059880

Regions

Nucleotide binding63 – 642NAD By similarity
Nucleotide binding131 – 1322NAD By similarity
Nucleotide binding172 – 1776NAD By similarity

Sites

Active site631Proton acceptor By similarity
Binding site1421NAD By similarity
Binding site1591NAD By similarity
Binding site1611NAD By similarity
Binding site1961NAD; via carbonyl oxygen By similarity
Binding site2301NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A3CU51 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: B2A5C11BB1101CBE

FASTA27029,135
        10         20         30         40         50         60 
MKIVLVSRID EADALRYTAS LARELEGLGH EVALEEGTAA HLGEGGISFE EIDGDLVVVV 

        70         80         90        100        110        120 
GGDGSVLLTV HQMKKQVPVL GINWGEVGFL ADLEPDEAGT FFAAHTEGFH IERRMRVSLS 

       130        140        150        160        170        180 
VNGVPLGDAL NEGLVVTDRP AKMLRFGVYV DGTPAERFRA DGLLVSTPTG STAYAMSAGG 

       190        200        210        220        230        240 
PIVDPQIEGF LLVPLAPYML SSRPHLISTG RNLEITLETE KPAHLVIDGQ STFELEKEAT 

       250        260        270 
LTVKKSDQPA LFVHTGKPFF EKVNHKLRNL 

« Hide

References

[1]"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1."
Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. expand/collapse author list , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.
Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000562 Genomic DNA. Translation: ABN56901.1.
RefSeqYP_001046883.1. NC_009051.1.

3D structure databases

ProteinModelPortalA3CU51.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING368407.Memar_0968.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN56901; ABN56901; Memar_0968.
GeneID4845934.
KEGGmem:Memar_0968.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMAHPSIPGW.

Enzyme and pathway databases

BioCycMMAR368407:GH7L-985-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_METMJ
AccessionPrimary (citable) accession number: A3CU51
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 20, 2007
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families