Reviewed, UniProtKB/Swiss-Prot A3CTG9 (GUAAA_METMJ)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Memar_0736

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_01510
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000294267
Regions
	Domain	3 – 184	182	Glutamine amidotransferase type-1
Sites
	Active site	75	1	Nucleophile By similarity
	Active site	162	1	By similarity
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3CTG9-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 86F8B26A50B931C0	FASTA	184	19,764
	10 20 30 40 50 60 MLPLYVVNNH GQFNHLILRT LRDMDIEATM ISNETPPAEV ARGCRGIVLG GGPTLERAGV 70 80 90 100 110 120 ASAYLDLGLP VLGICLGLHI MATARGGAIR RGASGGFGAV EVEIFEQNPL LRGYPDRMQV 130 140 150 160 170 180 WASHADEVSV VPEGFVRLAG SSICGVEAMA SPDEHLYGIQ WHPEVSHTVN GRLLFENFDG ICSE

References

[1]

"Complete sequence of Methanoculleus marisnigri JR1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Anderson I., Sieprawska-Lupa M., Whitman W., Woese C., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN56669.1.
RefSeq	YP_001046651.1.
3D structure databases
SMR	A3CTG9. Positions 4-183.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CTG9.
Genome annotation databases
GeneID	4846221.
GenomeReviews	Gene locus Memar_0736 in contig CP000562_GR.
KEGG	mem:Memar_0736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05587.
HOGENOM	HBG292341.
OMA	GQYVHRI.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR006220. Anth_synthII. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METMJ

Accession

Primary (citable) accession number: A3CTG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 20, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)