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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR368407:G1G85-395-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Memar_0403
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221561 – 1059Isoleucine--tRNA ligaseAdd BLAST1059

Proteomic databases

PRIDEiA3CSI6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_0403

Structurei

3D structure databases

ProteinModelPortaliA3CSI6
SMRiA3CSI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi591 – 595"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A3CSI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEVTGSYNP RELEAGVQDT WKRENTYARV QEVRKDGKAF FFVDGPPYTT
60 70 80 90 100
GHIHLGTAWN KIIKDTILRY HRMGGRNIIE RAGYDMHGLP IEVKVEHQLG
110 120 130 140 150
FTSKKDIEDY GIAAFIEQCR TFAVTHMEIM SEQFRQLGIW LDFDDPYQTI
160 170 180 190 200
KAEYIESAWW AVQRAEERGL LERGHRVVNW CPRCETAIAD SEVEYWDETD
210 220 230 240 250
PSIFVKFPVT GRENEYLVIW TTTPWTLPAN VAVAVSPAFT YARVAAKKDG
260 270 280 290 300
SEEILWIADE LVESVLKMGR YQDYTVLERV NGSDLVGTEY ESPLAGQVPH
310 320 330 340 350
QAEIRHRVVA ADYVALENTG LVHIAPGHGW DDYLIGIQEG LEAFCPVDAG
360 370 380 390 400
GCFTREAGAF ADMYVRDAND LVIDALGDYL LARRTITHRY GHCWRCKTSI
410 420 430 440 450
IYRATAQWFL KATEIREPML QEIAKVKWYP EWAGSARFHD FVRDSRDWCI
460 470 480 490 500
SRQRYWGIPI PIWQCEQCGE RTVIGTIAEL EERSGARVPD PHRPYVDEVV
510 520 530 540 550
IPCSCGGEMH RVADIFDVWF DSAVASWATL GFPREREAFD RLWPADFITE
560 570 580 590 600
GQDQTRGWFY SQLGASTVAF GRAPYKSVLM HGFALDADGR KMSKSFGNVV
610 620 630 640 650
TPEEVMNQFG VDVLRFYVLW ANAPWDDLKF NWDSVKTIHR TLNILWNVYR
660 670 680 690 700
FPLPYMVLDS FEPAAGDGGL WDGSFVRGNI NDMPEEDRWI ISRVNSLART
710 720 730 740 750
TAGDMQEYHL HRVTRALAAF ILEDLSRWYV QLVRPRMWLE EDSPEKRYAY
760 770 780 790 800
ETVYYVMRRL VALLAPFTPH IAEEIYGNLR LAGDPESVHM LDWPEADDLL
810 820 830 840 850
IAPDLESAME VVRSFDDAVA TARQNGRRKL RWPVAETVVV TGSDGVKTAL
860 870 880 890 900
EDLNDLALNR ANSRTVRVVT GRWDRILWQA EPVMRAIGPE FGKEGPKVKA
910 920 930 940 950
LIEGADGTAL KAAIERDGKA ELGGYEIAER HVTFAEALPE GVFAAPMKDA
960 970 980 990 1000
TVYVDVTLTP ALEAEGYARE VIRRIQEMRR QLDLNVDDFI VAAVDVADDR
1010 1020 1030 1040 1050
VASLIAEEEW QKEIAGEVRA AALTVRRTDG ERPTETFALE KDWDVEGVQM

QIGISRAGE
Length:1,059
Mass (Da):120,495
Last modified:March 20, 2007 - v1
Checksum:i9C554469148FCFD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA Translation: ABN56336.1
RefSeqiWP_011843246.1, NC_009051.1

Genome annotation databases

EnsemblBacteriaiABN56336; ABN56336; Memar_0403
GeneIDi4847697
KEGGimem:Memar_0403

Similar proteinsi

Entry informationi

Entry nameiSYI_METMJ
AccessioniPrimary (citable) accession number: A3CSI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health