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A3CSI6

- SYI_METMJ

UniProt

A3CSI6 - SYI_METMJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR368407:GH7L-409-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Memar_0403
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000002146: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10591059Isoleucine--tRNA ligasePRO_1000022156Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_0403.

Structurei

3D structure databases

ProteinModelPortaliA3CSI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CSI6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEVTGSYNP RELEAGVQDT WKRENTYARV QEVRKDGKAF FFVDGPPYTT
60 70 80 90 100
GHIHLGTAWN KIIKDTILRY HRMGGRNIIE RAGYDMHGLP IEVKVEHQLG
110 120 130 140 150
FTSKKDIEDY GIAAFIEQCR TFAVTHMEIM SEQFRQLGIW LDFDDPYQTI
160 170 180 190 200
KAEYIESAWW AVQRAEERGL LERGHRVVNW CPRCETAIAD SEVEYWDETD
210 220 230 240 250
PSIFVKFPVT GRENEYLVIW TTTPWTLPAN VAVAVSPAFT YARVAAKKDG
260 270 280 290 300
SEEILWIADE LVESVLKMGR YQDYTVLERV NGSDLVGTEY ESPLAGQVPH
310 320 330 340 350
QAEIRHRVVA ADYVALENTG LVHIAPGHGW DDYLIGIQEG LEAFCPVDAG
360 370 380 390 400
GCFTREAGAF ADMYVRDAND LVIDALGDYL LARRTITHRY GHCWRCKTSI
410 420 430 440 450
IYRATAQWFL KATEIREPML QEIAKVKWYP EWAGSARFHD FVRDSRDWCI
460 470 480 490 500
SRQRYWGIPI PIWQCEQCGE RTVIGTIAEL EERSGARVPD PHRPYVDEVV
510 520 530 540 550
IPCSCGGEMH RVADIFDVWF DSAVASWATL GFPREREAFD RLWPADFITE
560 570 580 590 600
GQDQTRGWFY SQLGASTVAF GRAPYKSVLM HGFALDADGR KMSKSFGNVV
610 620 630 640 650
TPEEVMNQFG VDVLRFYVLW ANAPWDDLKF NWDSVKTIHR TLNILWNVYR
660 670 680 690 700
FPLPYMVLDS FEPAAGDGGL WDGSFVRGNI NDMPEEDRWI ISRVNSLART
710 720 730 740 750
TAGDMQEYHL HRVTRALAAF ILEDLSRWYV QLVRPRMWLE EDSPEKRYAY
760 770 780 790 800
ETVYYVMRRL VALLAPFTPH IAEEIYGNLR LAGDPESVHM LDWPEADDLL
810 820 830 840 850
IAPDLESAME VVRSFDDAVA TARQNGRRKL RWPVAETVVV TGSDGVKTAL
860 870 880 890 900
EDLNDLALNR ANSRTVRVVT GRWDRILWQA EPVMRAIGPE FGKEGPKVKA
910 920 930 940 950
LIEGADGTAL KAAIERDGKA ELGGYEIAER HVTFAEALPE GVFAAPMKDA
960 970 980 990 1000
TVYVDVTLTP ALEAEGYARE VIRRIQEMRR QLDLNVDDFI VAAVDVADDR
1010 1020 1030 1040 1050
VASLIAEEEW QKEIAGEVRA AALTVRRTDG ERPTETFALE KDWDVEGVQM

QIGISRAGE
Length:1,059
Mass (Da):120,495
Last modified:March 20, 2007 - v1
Checksum:i9C554469148FCFD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN56336.1.
RefSeqiWP_011843246.1. NC_009051.1.
YP_001046318.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN56336; ABN56336; Memar_0403.
GeneIDi4847697.
KEGGimem:Memar_0403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA. Translation: ABN56336.1 .
RefSeqi WP_011843246.1. NC_009051.1.
YP_001046318.1. NC_009051.1.

3D structure databases

ProteinModelPortali A3CSI6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 368407.Memar_0403.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN56336 ; ABN56336 ; Memar_0403 .
GeneIDi 4847697.
KEGGi mem:Memar_0403.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MMAR368407:GH7L-409-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35101 / DSM 1498 / JR1.

Entry informationi

Entry nameiSYI_METMJ
AccessioniPrimary (citable) accession number: A3CSI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3