SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A3CSI6

- SYI_METMJ

UniProt

A3CSI6 - SYI_METMJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, Memar_0403
Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR368407:GH7L-409-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Memar_0403
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
ProteomesiUP000002146: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10591059Isoleucine--tRNA ligaseUniRule annotationPRO_1000022156Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_0403.

Structurei

3D structure databases

ProteinModelPortaliA3CSI6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotationAdd
BLAST
Motifi591 – 5955"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CSI6-1 [UniParc]FASTAAdd to Basket

« Hide

MKEVTGSYNP RELEAGVQDT WKRENTYARV QEVRKDGKAF FFVDGPPYTT     50
GHIHLGTAWN KIIKDTILRY HRMGGRNIIE RAGYDMHGLP IEVKVEHQLG 100
FTSKKDIEDY GIAAFIEQCR TFAVTHMEIM SEQFRQLGIW LDFDDPYQTI 150
KAEYIESAWW AVQRAEERGL LERGHRVVNW CPRCETAIAD SEVEYWDETD 200
PSIFVKFPVT GRENEYLVIW TTTPWTLPAN VAVAVSPAFT YARVAAKKDG 250
SEEILWIADE LVESVLKMGR YQDYTVLERV NGSDLVGTEY ESPLAGQVPH 300
QAEIRHRVVA ADYVALENTG LVHIAPGHGW DDYLIGIQEG LEAFCPVDAG 350
GCFTREAGAF ADMYVRDAND LVIDALGDYL LARRTITHRY GHCWRCKTSI 400
IYRATAQWFL KATEIREPML QEIAKVKWYP EWAGSARFHD FVRDSRDWCI 450
SRQRYWGIPI PIWQCEQCGE RTVIGTIAEL EERSGARVPD PHRPYVDEVV 500
IPCSCGGEMH RVADIFDVWF DSAVASWATL GFPREREAFD RLWPADFITE 550
GQDQTRGWFY SQLGASTVAF GRAPYKSVLM HGFALDADGR KMSKSFGNVV 600
TPEEVMNQFG VDVLRFYVLW ANAPWDDLKF NWDSVKTIHR TLNILWNVYR 650
FPLPYMVLDS FEPAAGDGGL WDGSFVRGNI NDMPEEDRWI ISRVNSLART 700
TAGDMQEYHL HRVTRALAAF ILEDLSRWYV QLVRPRMWLE EDSPEKRYAY 750
ETVYYVMRRL VALLAPFTPH IAEEIYGNLR LAGDPESVHM LDWPEADDLL 800
IAPDLESAME VVRSFDDAVA TARQNGRRKL RWPVAETVVV TGSDGVKTAL 850
EDLNDLALNR ANSRTVRVVT GRWDRILWQA EPVMRAIGPE FGKEGPKVKA 900
LIEGADGTAL KAAIERDGKA ELGGYEIAER HVTFAEALPE GVFAAPMKDA 950
TVYVDVTLTP ALEAEGYARE VIRRIQEMRR QLDLNVDDFI VAAVDVADDR 1000
VASLIAEEEW QKEIAGEVRA AALTVRRTDG ERPTETFALE KDWDVEGVQM 1050
QIGISRAGE 1059
Length:1,059
Mass (Da):120,495
Last modified:March 20, 2007 - v1
Checksum:i9C554469148FCFD1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000562 Genomic DNA. Translation: ABN56336.1.
RefSeqiWP_011843246.1. NC_009051.1.
YP_001046318.1. NC_009051.1.

Genome annotation databases

EnsemblBacteriaiABN56336; ABN56336; Memar_0403.
GeneIDi4847697.
KEGGimem:Memar_0403.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000562 Genomic DNA. Translation: ABN56336.1 .
RefSeqi WP_011843246.1. NC_009051.1.
YP_001046318.1. NC_009051.1.

3D structure databases

ProteinModelPortali A3CSI6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 368407.Memar_0403.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN56336 ; ABN56336 ; Memar_0403 .
GeneIDi 4847697.
KEGGi mem:Memar_0403.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MMAR368407:GH7L-409-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35101 / DSM 1498 / JR1.

Entry informationi

Entry nameiSYI_METMJ
AccessioniPrimary (citable) accession number: A3CSI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi