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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58Substrate; via amide nitrogenUniRule annotation1
Binding sitei134ATPUniRule annotation1
Binding sitei180ATPUniRule annotation1
Binding sitei207Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR368407:G1G85-251-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Memar_0262
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003839161 – 279Hydroxyethylthiazole kinaseAdd BLAST279

Interactioni

Protein-protein interaction databases

STRINGi368407.Memar_0262

Structurei

3D structure databases

ProteinModelPortaliA3CS46
SMRiA3CS46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00019 Archaea
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG093Z0A6M

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

A3CS46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDNTREGSS ARFVDGAIPA GLLAAVRSQR PLVHHITNGV TINDCANITI
60 70 80 90 100
CAGAAPVMAE APEEVAGMVA AAGALVLNIG TLSAAQVEAM LIAGRRANEL
110 120 130 140 150
GIPVVLDPVG VGATEFRTAT ARKLLDTLDI AVLKGNAGEI GVLAGTGGSV
160 170 180 190 200
RGVDSGGVAG DPVETARECA RSTGTVVSMT GAVDVVTDGS RVFLVGNGNP
210 220 230 240 250
AMDRLSGTGC MASSVTAAFV AVAEDYAVAS AAALAAFGLA GEWGAAGARG
260 270
PYTFRTALFD ELAGLSPDDL AGHARIEER
Length:279
Mass (Da):27,828
Last modified:March 20, 2007 - v1
Checksum:iC70D21965BB467F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA Translation: ABN56196.1
RefSeqiWP_011843117.1, NC_009051.1

Genome annotation databases

EnsemblBacteriaiABN56196; ABN56196; Memar_0262
GeneIDi4847849
KEGGimem:Memar_0262

Similar proteinsi

Entry informationi

Entry nameiTHIM_METMJ
AccessioniPrimary (citable) accession number: A3CS46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: March 20, 2007
Last modified: April 25, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health