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A3CS12 (A3CS12_METMJ) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Ordered Locus Names:Memar_0228
OrganismMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]
Taxonomic identifier368407 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site21For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2381Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding3841Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4351Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4381Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
A3CS12 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: FF2236FF54ECC672

FASTA47852,362
        10         20         30         40         50         60 
MCGIVGIVDA GGVSFPLYYA LYALQHRGQE SAGISTFEGT TLYTHKAQGL VAEVFNSQTL 

        70         80         90        100        110        120 
QDLRGNAGIG HVRYPTTGSK VPENVQPFNF RYRGLDLSIA HNGNLVNTVE LREEYERRGQ 

       130        140        150        160        170        180 
IFCTTTDTEI IGNIIADALR TSKSMEDAVL LCMRRLRGSY ATVALLNNTV YAFRDPLGIK 

       190        200        210        220        230        240 
PLCIGKLDNG YIVASESVAI DALDGTFVRD VRPGELVRID ESGLTSTQIA TANRKAYCIF 

       250        260        270        280        290        300 
EYVYFARADS VMDGTLVYDV RRRIGQKLYD ANPVEADTAC PVPDSGIAYA AGYAEQSGIP 

       310        320        330        340        350        360 
FIEGLMKNRY MGRTFIMPTQ EQRERAVRIK LNTVRGNLKD KRVVLIDDSV VRGTTSRRIV 

       370        380        390        400        410        420 
NMIRDAGAEE IHLRVGSPPI IAPCYLGVDM PTRTELIASG KEVEAVRESV GATSLTYIPL 

       430        440        450        460        470 
DDLVEAIGCG ERNLCTGCLT GCYPVEINGE KSCHSIVDYV AGTHQSDLST FKAGKERT

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References

[1]"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1."
Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. expand/collapse author list , Lucas S., Richardson P., Whitman W., Kyrpides N.C.
Stand. Genomic Sci. 1:189-196(2009) [PubMed: 21304656] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000562 Genomic DNA. Translation: ABN56162.1.
RefSeqYP_001046144.1. NC_009051.1.

3D structure databases

ProteinModelPortalA3CS12.
ModBaseSearch...

Protein-protein interaction databases

STRINGA3CS12.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4847844.
GenomeReviewsGene locus Memar_0228 in contig CP000562_GR.
KEGGmem:Memar_0228.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGarNOG04070.
HOGENOMHBG392416.
OMADSMFEDQ.
PhylomeDBA3CS12.
ProtClustDBCLSK964665.

Family and domain databases

InterProIPR005854. Amd_phspho_trans.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR000836. PRibTrfase.
[Graphical view]
KOK00764.
PANTHERPTHR11907. Amd_phspho_trans. 1 hit.
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMsTIGR01134. PurF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA3CS12_METMJ
AccessionPrimary (citable) accession number: A3CS12
Entry history
Integrated into UniProtKB/TrEMBL: March 20, 2007
Last sequence update: March 20, 2007
Last modified: January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info