A3CRT9 (ARGB_METMJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	Memar_0154

Organism

Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) [Complete proteome] [HAMAP]

Taxonomic identifier

368407 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Methanomicrobiaceae › Methanoculleus › Methanoculleus marisnigri

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP-Rule MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP-Rule MF_00082
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Acetylglutamate kinase HAMAP-Rule MF_00082		PRO_1000010509
Regions
	Region	60 – 61	2	Substrate binding By similarity
Sites
	Binding site	82	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Site	25	1	Transition state stabilizer By similarity
	Site	247	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A3CRT9 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 0623127845D9795F	FASTA	289	30,949
	10 20 30 40 50 60 MKREDVLMEA LPYIQKFYGK TIVIKLGGHA MVDQSILETV IRDAVLLRYV GMKVVLVHGG 70 80 90 100 110 120 GPEITAKMQA MGKEPKFVGG LRITDPETLE IAQMVLVGKI NDGIVSLIAN CGTRAVGISG 130 140 150 160 170 180 NDGNLLIARK MDPQRVQVGE VMQEVDLGQV GEIEEVDPEV LHCLLAQNYI PVVAPIAIDR 190 200 210 220 230 240 QGMSLNINAD TAAAEIAIAL SAFKLVNLTD VDGVMDADRT MVYHRLALTE AEAMIGAGVI 250 260 270 280 AGGMIPKLEG CMKAVRNGVA SAHVVNGNRE HNLLLELFTD QGVGTMLTL

References

[1]

"Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981 type strain JR1." Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A., Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C., Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L., Land M. , Lucas S., Richardson P., Whitman W.B., Kyrpides N.C. Stand. Genomic Sci. 1:189-196(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35101 / DSM 1498 / JR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000562 Genomic DNA. Translation: ABN56089.1.
RefSeq	YP_001046071.1. NC_009051.1.
3D structure databases
ProteinModelPortal	A3CRT9.
ModBase	Search...
Protein-protein interaction databases
STRING	368407.Memar_0154.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN56089; ABN56089; Memar_0154.
GeneID	4848336.
KEGG	mem:Memar_0154.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HOG000233259.
KO	K00930.
OMA	GNMVFAE.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	MMAR368407:GH7L-207-MONOMER.
UniPathway	UPA00068; UER00107.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00082_A. ArgB_A.
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_METMJ

Accession

Primary (citable) accession number: A3CRT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 20, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program