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A3CQ23 (TRMB_STRSV) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SSA_1902
OrganismStreptococcus sanguinis (strain SK36) [Complete proteome] [HAMAP]
Taxonomic identifier388919 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 212212tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288240

Regions

Region124 – 1296Interaction with RNA Potential
Region191 – 1944Substrate binding By similarity

Sites

Active site1181 By similarity
Binding site441S-adenosyl-L-methionine By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1181S-adenosyl-L-methionine By similarity
Binding site1221Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A3CQ23 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 34DF75E71EED349F

FASTA21224,395
        10         20         30         40         50         60 
MRVRNRKGAT ELLEAHPQYV ILNPADAKGR WQEIFGNDHP IHVEVGSGKG AFVSGMAKAN 

        70         80         90        100        110        120 
PEINYIGIDI QKSVLSYALD KVLATDVPNI KLLWVDGSDL TDYFEEGEID RLYLNFSDPW 

       130        140        150        160        170        180 
PKKRHEKRRL TYQSFLATYQ QILPENGEIH FKTDNRGLFE YSLVSFSQYG MKLKGVWLDL 

       190        200        210 
HASDFEDNVL TEYEQKFANK GQVIYRVEAA FE 

« Hide

References

[1]"Genome of the opportunistic pathogen Streptococcus sanguinis."
Xu P., Alves J.M., Kitten T., Brown A., Chen Z., Ozaki L.S., Manque P., Ge X., Serrano M.G., Puiu D., Hendricks S., Wang Y., Chaplin M.D., Akan D., Paik S., Peterson D.L., Macrina F.L., Buck G.A.
J. Bacteriol. 189:3166-3175(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SK36.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000387 Genomic DNA. Translation: ABN45278.1.
RefSeqYP_001035828.1. NC_009009.1.

3D structure databases

ProteinModelPortalA3CQ23.
SMRA3CQ23. Positions 6-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING388919.SSA_1902.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN45278; ABN45278; SSA_1902.
GeneID4806278.
KEGGssa:SSA_1902.
PATRIC19770723. VBIStrSan33173_1803.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMADNVMTEY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycSSAN388919:GHEN-1872-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_STRSV
AccessionPrimary (citable) accession number: A3CQ23
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 20, 2007
Last modified: June 11, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways