A3CN34 (A3CN34_STRSV) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I, putative EMBL ABN44589.1
EC=5.99.1.2 EMBL ABN44589.1

Gene names

Name:	topA EMBL ABN44589.1
Ordered Locus Names:	SSA_1184

Organism

Streptococcus sanguinis (strain SK36) [Complete proteome] [HAMAP] EMBL ABN44589.1

Taxonomic identifier

388919 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A3CN34 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: ACE4F578DC826F54	FASTA	697	79,209
	10 20 30 40 50 60 MSKLVTKTKK KSTTKKNLVI VESPAKAKTI EKYLGRNYKV LASVGHIRDL KKSTMSIDFE 70 80 90 100 110 120 NNYEPQYINI RGKGPLINDL KKEAKKAKQV FLASDPDREG EAISWHLAHI LNLDEKEKNR 130 140 150 160 170 180 VVFNEITKDA VKNAFKDPRQ IDMDLVDAQQ ARRVLDRLVG YSISPILWKK VKKGLSAGRV 190 200 210 220 230 240 QSVALKLIID RENEINAFKP EEYWTIDGTF KKGTRQFQAS FYGMNGKKMK LTNNEYVKEV 250 260 270 280 290 300 LSHLKGDDFT VDSVEKKERR RNAPLPYTTS SMQQDAANKI NFRTRKTMMV AQQLYEGINI 310 320 330 340 350 360 GSGVQGLITY MRTDSTRISP VAQNEAASFI TDKFGAKYSK HGSKAKNASG AQDAHEAIRP 370 380 390 400 410 420 SSVFNTPESI AKYLDKDQLK LYTLIWNRFV ASQMTAAVFD TMNVKLGQNG VQFAANGSQV 430 440 450 460 470 480 KFDGYLAIYN DSDKNKMLPD MEKGDTVKRV NTNPEQHFTQ PPARYSEATL IKTLEENGVG 490 500 510 520 530 540 RPSTYAPTIE TIQKRYYVKL VSKRFEPTEL GEIVNSLIVE FFPGIVNVKF TAEMEAKLDD 550 560 570 580 590 600 VEVGKEQWQK VIDEFYRPFE KEVAKAETEM EKIQIKDEPA GFDCEVCGSP MVIKLGRFGK 610 620 630 640 650 660 FYACSNFPDC RHTQAIVKEI GVTCPQCQKG QVIERKTKRN RIFYGCDRYP DCDFTSWDKP 670 680 690 VGRNCPKCDH YLIEKKVRGG GKQVVCSNGD YEEEKVK

References

[1]

"Genome of the opportunistic pathogen Streptococcus sanguinis." Xu P., Alves J.M., Kitten T., Brown A., Chen Z., Ozaki L.S., Manque P., Ge X., Serrano M.G., Puiu D., Hendricks S., Wang Y., Chaplin M.D., Akan D., Paik S., Peterson D.L., Macrina F.L., Buck G.A. J. Bacteriol. 189:3166-3175(2007) [PubMed: 17277061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000387 Genomic DNA. Translation: ABN44589.1.
RefSeq	YP_001035139.1. NC_009009.1.
3D structure databases
ProteinModelPortal	A3CN34.
ModBase	Search...
Protein-protein interaction databases
STRING	A3CN34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000052749; EBSTRP00000049013; EBSTRG00000052739.
GeneID	4807818.
GenomeReviews	Gene locus SSA_1184 in contig CP000387_GR.
KEGG	ssa:SSA_1184.
NMPDR	fig|388919.8.peg.1062.
PATRIC	19769330. VBIStrSan33173_1126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000027862.
HOGENOM	HBG512935.
OMA	PREIDIK.
PhylomeDB	A3CN34.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A3CN34_STRSV

Accession

Primary (citable) accession number: A3CN34

Entry history

Integrated into UniProtKB/TrEMBL:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)