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A3CLP4

- SYI_STRSV

UniProt

A3CLP4 - SYI_STRSV

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SSA_0661
Organism
Streptococcus sanguinis (strain SK36)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity
Metal bindingi888 – 8881Zinc By similarity
Metal bindingi891 – 8911Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSAN388919:GHEN-670-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SSA_0661
OrganismiStreptococcus sanguinis (strain SK36)
Taxonomic identifieri388919 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000002148: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022132Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi388919.SSA_0661.

Structurei

3D structure databases

ProteinModelPortaliA3CLP4.
SMRiA3CLP4. Positions 1-917.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CLP4-1 [UniParc]FASTAAdd to Basket

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MKLKETLNLG KTAFPMRAGL PNKEPIWQKE WDQAKLYQRR QELNEGKPHF    50
ILHDGPPYAN GNIHVGHAMN KISKDIIVRS KSMAGYYAPY IPGWDTHGLP 100
IEQVLAKQGV KRKELDLVEY LNMCRDYALS QVEKQKEDFK RLGVSGDWEN 150
PYVTLTPDYE AAQIRVFGEM AKKGYIYRGA KPVYWSWSSE SALAEAEIEY 200
HDLVSTSLYY ANRVKDGKGV LDTDTYIVVW TTTPFTITAS RGLTVGADID 250
YVVVQPAGES RKFVVASELL NSLSEKFGWA DVQVLATYRG QELNHIVTVH 300
PWDTAVDELV ILGDHVTTDS GTGIVHTAPG FGEDDYNVGV ANDLEVFVTV 350
NERGIMMENA GPDFAGQFYD KVAPTVIEKL GDLLLAQEEI SHSYPFDWRT 400
KKPIIWRAVP QWFASVSKFR QEILDEIEKV KFHSEWGKVR LYNMIRDRGD 450
WVISRQRAWG VPLPIFYAED GTPIMTAETI EHVAQLFEEH GSIVWWKREA 500
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSWNGVVV NRPELTYPAD 550
LYLEGSDQYR GWFNSSLITS VANHGVAPYK QILSQGFALD GKGEKMSKSL 600
GNTIAPSDVE KQFGAEILRL WVTSVDSSND VRISMDILSQ VSETYRKIRN 650
TLRFLIANTS DFNPVEDAVA YEELRSVDKY MTIRFNQLVE TIRKAYADFE 700
FLTIYKAIVN FVTVDLSAFY LDFAKDIVYI EAAKSLERRQ MQTVFYDVLV 750
KITKLLTPIL PHTAEEIWSY LEHEEEEFVQ LSELPEAQTF PNQEEILDTW 800
SAFMDFRSQA QKALEEARNE KVIGKSLEAH LTVYPNEVIK TLLEAVNSDV 850
AQLLIVSQLT IAEGPAPEGA LVFEDVAFVV EHAQGQVCDR CRRIDTTVQE 900
RSYQALVCDH CAEILEENFA QAVSEGFEAN 930
Length:930
Mass (Da):105,550
Last modified:March 20, 2007 - v1
Checksum:i8C7E2BCC6478234E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000387 Genomic DNA. Translation: ABN44099.1.
RefSeqiWP_011836649.1. NC_009009.1.
YP_001034649.1. NC_009009.1.

Genome annotation databases

EnsemblBacteriaiABN44099; ABN44099; SSA_0661.
GeneIDi4806788.
KEGGissa:SSA_0661.
PATRICi19768350. VBIStrSan33173_0637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000387 Genomic DNA. Translation: ABN44099.1 .
RefSeqi WP_011836649.1. NC_009009.1.
YP_001034649.1. NC_009009.1.

3D structure databases

ProteinModelPortali A3CLP4.
SMRi A3CLP4. Positions 1-917.
ModBasei Search...

Protein-protein interaction databases

STRINGi 388919.SSA_0661.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN44099 ; ABN44099 ; SSA_0661 .
GeneIDi 4806788.
KEGGi ssa:SSA_0661.
PATRICi 19768350. VBIStrSan33173_0637.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSAN388919:GHEN-670-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SK36.

Entry informationi

Entry nameiSYI_STRSV
AccessioniPrimary (citable) accession number: A3CLP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi