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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Streptococcus sanguinis (strain SK36)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771Charge relay systemUniRule annotation
Active sitei152 – 1521Charge relay systemUniRule annotation
Active sitei176 – 1761Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSAN388919:GHEN-584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:SSA_0570
OrganismiStreptococcus sanguinis (strain SK36)
Taxonomic identifieri388919 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002148 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Glutamyl-tRNA(Gln) amidotransferase subunit APRO_1000015917Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi388919.SSA_0570.

Structurei

3D structure databases

ProteinModelPortaliA3CLF8.
SMRiA3CLF8. Positions 1-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A3CLF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFNHKTIEE LHDLLVKKEI SAVELTQATL ADIKEREAAV DSFITVSEEE
60 70 80 90 100
ALAQAAALDA KGIDADNLMS GIPLAVKDNI STKGILTTAA SKILYNYKPI
110 120 130 140 150
FDATSVEKLY GKDMIIVGKT NMDEFAMGGS SENSYFKTTK NAWDSSKVPG
160 170 180 190 200
GSSGGSATAV ASGQVRLSLG SDTGGSIRQP ASFNGVVGLK PTYGRVSRFG
210 220 230 240 250
LIAFGSSLDQ IGPFSQTVKE NAQLLNVISG NDPKDSTSSQ EEVPDFTSKI
260 270 280 290 300
GQDIKGMKIA LPKEYMGEGI DSKVKETILA AAKHLESLGA IVEEVSLPHS
310 320 330 340 350
KYGVAVYYII ASSEASSNLQ RFDGIRYGYR AEGIENLEDV YVKSRSEGFG
360 370 380 390 400
EEVKRRIMLG TFSLSSGYYD AYFKKAGQVR TLIMQDFAKV FEKYDLILGP
410 420 430 440 450
TAPTVAYDLG SQNQDPVAMY LADLLTIPVN LAGLPGISIP AGFVDGLPVG
460 470 480
LQLIGNHFDE ATIYQTAAAF EATTDYHKQQ PVIFGGEK
Length:488
Mass (Da):52,414
Last modified:March 20, 2007 - v1
Checksum:iFDB0429FDF2D09C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000387 Genomic DNA. Translation: ABN44013.1.
RefSeqiWP_011836596.1. NC_009009.1.
YP_001034563.1. NC_009009.1.

Genome annotation databases

EnsemblBacteriaiABN44013; ABN44013; SSA_0570.
GeneIDi4807934.
KEGGissa:SSA_0570.
PATRICi19768174. VBIStrSan33173_0549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000387 Genomic DNA. Translation: ABN44013.1.
RefSeqiWP_011836596.1. NC_009009.1.
YP_001034563.1. NC_009009.1.

3D structure databases

ProteinModelPortaliA3CLF8.
SMRiA3CLF8. Positions 1-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi388919.SSA_0570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN44013; ABN44013; SSA_0570.
GeneIDi4807934.
KEGGissa:SSA_0570.
PATRICi19768174. VBIStrSan33173_0549.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiHASPLEM.

Enzyme and pathway databases

BioCyciSSAN388919:GHEN-584-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_STRSV
AccessioniPrimary (citable) accession number: A3CLF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 20, 2007
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.