ID   FTHS1_STRSV             Reviewed;         557 AA.
AC   A3CL27;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   24-JAN-2024, entry version 84.
DE   RecName: Full=Formate--tetrahydrofolate ligase 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS 1 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs1 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=SSA_0432;
OS   Streptococcus sanguinis (strain SK36).
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=388919;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SK36;
RX   PubMed=17277061; DOI=10.1128/jb.01808-06;
RA   Xu P., Alves J.M., Kitten T., Brown A., Chen Z., Ozaki L.S., Manque P.,
RA   Ge X., Serrano M.G., Puiu D., Hendricks S., Wang Y., Chaplin M.D., Akan D.,
RA   Paik S., Peterson D.L., Macrina F.L., Buck G.A.;
RT   "Genome of the opportunistic pathogen Streptococcus sanguinis.";
RL   J. Bacteriol. 189:3166-3175(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABN43882.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000387; ABN43882.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011836509.1; NC_009009.1.
DR   RefSeq; YP_001034432.2; NC_009009.1.
DR   AlphaFoldDB; A3CL27; -.
DR   SMR; A3CL27; -.
DR   STRING; 388919.SSA_0432; -.
DR   KEGG; ssa:SSA_0432; -.
DR   PATRIC; fig|388919.9.peg.418; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002148; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..557
FT                   /note="Formate--tetrahydrofolate ligase 1"
FT                   /id="PRO_0000293069"
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   557 AA;  59000 MW;  E84BD4012405BAEC CRC64;
     MVLSDIEIAN SVQMKSIKEV AEKLGIAEDA LSLYGNYKAK ISAGQLEALK DKPDGKLILV
     TAISPTPAGE GKTTTSVGLV DALAAIGKKA VIALREPSLG PVFGIKGGAA GGGHAQVVPM
     EDINLHFTGD FHAIGVANNL LAALIDNHIH HGNALGIDSR RITWKRAVDM NDRQLRHIVD
     GLQGKVNGVP REDGFDITVA SEVMAILCLS ENITDLKNRL EKIIIGYSFE GKPVTAKDLK
     AGGAMAAVLK DAIHPNLVQT LEHTPALIHG GPFANIAHGC NSVLATKLAL KYADYAVTEA
     GFGADLGAEK FIDIKCRTSG LRPAAVVLVA TIRALKMHGG VAKSDLAEEN VQAVVDGLPN
     LEKHLENIQD VYGLPAVVAI NKFPLDTEAE LQAVYDACQK RGVDVVISDV WANGGAGGKE
     LAEKVVELAE GDNHFQFVYN EEDSIETKLN KIVTKVYGGK GVRLTPAAKR ELKQLDELGF
     SNYPICMAKT QYSFSDDAKK LGAPKDFVVT ISQLKVSAGA GFIVALTGAI MTMPGLPKVP
     ASEKIDVDKD GNISGLF
//