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A3BMZ5 (BGL26_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 26

Short name=Os7bglu26
EC=3.2.1.21
Gene names
Name:BGLU26
Ordered Locus Names:Os07g0656200, LOC_Os07g46280
ORF Names:OsJ_25416
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides and sophorose. Ref.5

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0) Ref.5

KM=0.52 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)

KM=19.6 mM for cellobiose (at pH 5.0)

KM=0.52 mM for cellotriose (at pH 5.0)

KM=0.09 mM for cellotetraose (at pH 5.0)

KM=0.06 mM for cellopentaose (at pH 5.0)

KM=0.05 mM for cellohexaose (at pH 5.0)

KM=0.86 mM for laminaribiose (at pH 5.0)

KM=8.7 mM for laminaritriose (at pH 5.0)

Sequence caution

The sequence BAF22416.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 510483Beta-glucosidase 26
PRO_0000390343

Regions

Region470 – 4712Substrate binding By similarity

Sites

Active site2061Proton donor By similarity
Active site4161Nucleophile By similarity
Binding site591Substrate By similarity
Binding site1601Substrate By similarity
Binding site2051Substrate By similarity
Binding site3451Substrate By similarity
Binding site4631Substrate By similarity

Amino acid modifications

Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation1271N-linked (GlcNAc...) Potential
Glycosylation2331N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Disulfide bond225 ↔ 228 By similarity

Sequences

Sequence LengthMass (Da)Tools
A3BMZ5 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 9EBEAE24EE62833E

FASTA51058,498
        10         20         30         40         50         60 
MRKFIAALRL ALAAAAHLLL TLPPAQCYWL NPEIYDAGGL SRRAFPEGFV FGTAASAYQV 

        70         80         90        100        110        120 
EGMAKQGGRG PSIWDAFIEK PGTIPNNATA DVTVDEYHRY KEDVNIMKNM GFDAYRFSIS 

       130        140        150        160        170        180 
WSRIFPNGTG MVNQEGVDYY NRLIDYMVKK GIKPYANLYH YDLPLALHEQ YLGWLSPNIV 

       190        200        210        220        230        240 
EAFADYADFC FQTFGDRVKD WFTFNEPRCV AALGYDNGFH APGRCSGCDA GGNSTTEPYL 

       250        260        270        280        290        300 
AAHHLILSHA AAVKRYREKY QLYQKGRIGI LLDFVWYEPF SDSNADRAAA QRARDFHLGW 

       310        320        330        340        350        360 
FLDPIIHGRY PYSMLEIVKD RMPTFSDEES RMVKDSIDYV GINHYTSFYM KDPGPWNLTP 

       370        380        390        400        410        420 
TSYQDDWHVG FAYERNGVPI GAQANSYWLY IVPWGINKAV TYVKETYGNP TMILSENGMD 

       430        440        450        460        470        480 
QPGNVSITQG VHDTVRIRYY RNYITELKKA IDDGAKVIGY FAWSLLDNFE WRLGYTSRFG 

       490        500        510 
IVYVDYKTLK RYPKDSAFWF KNMLSSKKRN 

« Hide

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Rice family GH1 glycoside hydrolases with beta-D-glucosidase and beta-D-mannosidase activities."
Kuntothom T., Luang S., Harvey A.J., Fincher G.B., Opassiri R., Hrmova M., Ketudat Cairns J.R.
Arch. Biochem. Biophys. 491:85-95(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008213 Genomic DNA. Translation: BAF22416.1. Sequence problems.
CM000144 Genomic DNA. Translation: EAZ40934.1.
RefSeqNP_001060502.1. NM_001067037.1.
UniGeneOs.20617.

3D structure databases

ProteinModelPortalA3BMZ5.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEA3BMZ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4344146.
KEGGosa:4344146.

Organism-specific databases

GrameneA3BMZ5.

Phylogenomic databases

eggNOGCOG2723.

Enzyme and pathway databases

SABIO-RKA3BMZ5.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGL26_ORYSJ
AccessionPrimary (citable) accession number: A3BMZ5
Secondary accession number(s): Q0D407
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries