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Protein

Beta-glucosidase 26

Gene

BGLU26

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides and sophorose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
  2. KM=0.52 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)1 Publication
  3. KM=19.6 mM for cellobiose (at pH 5.0)1 Publication
  4. KM=0.52 mM for cellotriose (at pH 5.0)1 Publication
  5. KM=0.09 mM for cellotetraose (at pH 5.0)1 Publication
  6. KM=0.06 mM for cellopentaose (at pH 5.0)1 Publication
  7. KM=0.05 mM for cellohexaose (at pH 5.0)1 Publication
  8. KM=0.86 mM for laminaribiose (at pH 5.0)1 Publication
  9. KM=8.7 mM for laminaritriose (at pH 5.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei59 – 591SubstrateBy similarity
    Binding sitei160 – 1601SubstrateBy similarity
    Binding sitei205 – 2051SubstrateBy similarity
    Active sitei206 – 2061Proton donorBy similarity
    Binding sitei345 – 3451SubstrateBy similarity
    Active sitei416 – 4161NucleophileBy similarity
    Binding sitei463 – 4631SubstrateBy similarity

    GO - Molecular functioni

    • beta-D-fucosidase activity Source: UniProtKB
    • beta-galactosidase activity Source: UniProtKB
    • beta-glucosidase activity Source: UniProtKB
    • beta-L-arabinosidase activity Source: UniProtKB
    • beta-mannosidase activity Source: UniProtKB
    • cellobiose glucosidase activity Source: UniProtKB
    • glucan exo-1,3-beta-glucosidase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKA3BMZ5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 26 (EC:3.2.1.21)
    Short name:
    Os7bglu26
    Gene namesi
    Name:BGLU26
    Ordered Locus Names:Os07g0656200, LOC_Os07g46280
    ORF Names:OsJ_25416
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763 Componenti: Chromosome 7

    Organism-specific databases

    GrameneiA3BMZ5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 510483Beta-glucosidase 26PRO_0000390343Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi225 ↔ 228By similarity
    Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiA3BMZ5.

    Structurei

    3D structure databases

    ProteinModelPortaliA3BMZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni470 – 4712Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    InParanoidiA3BMZ5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A3BMZ5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRKFIAALRL ALAAAAHLLL TLPPAQCYWL NPEIYDAGGL SRRAFPEGFV
    60 70 80 90 100
    FGTAASAYQV EGMAKQGGRG PSIWDAFIEK PGTIPNNATA DVTVDEYHRY
    110 120 130 140 150
    KEDVNIMKNM GFDAYRFSIS WSRIFPNGTG MVNQEGVDYY NRLIDYMVKK
    160 170 180 190 200
    GIKPYANLYH YDLPLALHEQ YLGWLSPNIV EAFADYADFC FQTFGDRVKD
    210 220 230 240 250
    WFTFNEPRCV AALGYDNGFH APGRCSGCDA GGNSTTEPYL AAHHLILSHA
    260 270 280 290 300
    AAVKRYREKY QLYQKGRIGI LLDFVWYEPF SDSNADRAAA QRARDFHLGW
    310 320 330 340 350
    FLDPIIHGRY PYSMLEIVKD RMPTFSDEES RMVKDSIDYV GINHYTSFYM
    360 370 380 390 400
    KDPGPWNLTP TSYQDDWHVG FAYERNGVPI GAQANSYWLY IVPWGINKAV
    410 420 430 440 450
    TYVKETYGNP TMILSENGMD QPGNVSITQG VHDTVRIRYY RNYITELKKA
    460 470 480 490 500
    IDDGAKVIGY FAWSLLDNFE WRLGYTSRFG IVYVDYKTLK RYPKDSAFWF
    510
    KNMLSSKKRN
    Length:510
    Mass (Da):58,498
    Last modified:March 20, 2007 - v1
    Checksum:i9EBEAE24EE62833E
    GO

    Sequence cautioni

    The sequence BAF22416.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP008213 Genomic DNA. Translation: BAF22416.1. Sequence problems.
    CM000144 Genomic DNA. Translation: EAZ40934.1.
    RefSeqiNP_001060502.1. NM_001067037.1.
    UniGeneiOs.20617.

    Genome annotation databases

    GeneIDi4344146.
    KEGGiosa:4344146.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP008213 Genomic DNA. Translation: BAF22416.1. Sequence problems.
    CM000144 Genomic DNA. Translation: EAZ40934.1.
    RefSeqiNP_001060502.1. NM_001067037.1.
    UniGeneiOs.20617.

    3D structure databases

    ProteinModelPortaliA3BMZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiA3BMZ5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    GeneIDi4344146.
    KEGGiosa:4344146.

    Organism-specific databases

    GrameneiA3BMZ5.

    Phylogenomic databases

    eggNOGiCOG2723.
    InParanoidiA3BMZ5.

    Enzyme and pathway databases

    SABIO-RKA3BMZ5.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
      Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
      BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Rice family GH1 glycoside hydrolases with beta-D-glucosidase and beta-D-mannosidase activities."
      Kuntothom T., Luang S., Harvey A.J., Fincher G.B., Opassiri R., Hrmova M., Ketudat Cairns J.R.
      Arch. Biochem. Biophys. 491:85-95(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiBGL26_ORYSJ
    AccessioniPrimary (citable) accession number: A3BMZ5
    Secondary accession number(s): Q0D407
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: March 20, 2007
    Last modified: January 7, 2015
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.