Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A3BMZ5

- BGL26_ORYSJ

UniProt

A3BMZ5 - BGL26_ORYSJ

Protein

Beta-glucosidase 26

Gene

BGLU26

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (20 Mar 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides, laminari-oligosaccharides and sophorose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Kineticsi

    1. KM=0.27 mM for p-nitrophenyl beta-D-glucoside (at pH 5.0)1 Publication
    2. KM=0.52 mM for p-nitrophenyl beta-D-mannoside (at pH 5.0)1 Publication
    3. KM=19.6 mM for cellobiose (at pH 5.0)1 Publication
    4. KM=0.52 mM for cellotriose (at pH 5.0)1 Publication
    5. KM=0.09 mM for cellotetraose (at pH 5.0)1 Publication
    6. KM=0.06 mM for cellopentaose (at pH 5.0)1 Publication
    7. KM=0.05 mM for cellohexaose (at pH 5.0)1 Publication
    8. KM=0.86 mM for laminaribiose (at pH 5.0)1 Publication
    9. KM=8.7 mM for laminaritriose (at pH 5.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei59 – 591SubstrateBy similarity
    Binding sitei160 – 1601SubstrateBy similarity
    Binding sitei205 – 2051SubstrateBy similarity
    Active sitei206 – 2061Proton donorBy similarity
    Binding sitei345 – 3451SubstrateBy similarity
    Active sitei416 – 4161NucleophileBy similarity
    Binding sitei463 – 4631SubstrateBy similarity

    GO - Molecular functioni

    1. beta-D-fucosidase activity Source: UniProtKB
    2. beta-galactosidase activity Source: UniProtKB
    3. beta-glucosidase activity Source: UniProtKB
    4. beta-L-arabinosidase activity Source: UniProtKB
    5. beta-mannosidase activity Source: UniProtKB
    6. cellobiose glucosidase activity Source: UniProtKB
    7. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKA3BMZ5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 26 (EC:3.2.1.21)
    Short name:
    Os7bglu26
    Gene namesi
    Name:BGLU26
    Ordered Locus Names:Os07g0656200, LOC_Os07g46280
    ORF Names:OsJ_25416
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 7

    Organism-specific databases

    GrameneiA3BMZ5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 510483Beta-glucosidase 26PRO_0000390343Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi225 ↔ 228By similarity
    Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiA3BMZ5.

    Structurei

    3D structure databases

    ProteinModelPortaliA3BMZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni470 – 4712Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A3BMZ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKFIAALRL ALAAAAHLLL TLPPAQCYWL NPEIYDAGGL SRRAFPEGFV    50
    FGTAASAYQV EGMAKQGGRG PSIWDAFIEK PGTIPNNATA DVTVDEYHRY 100
    KEDVNIMKNM GFDAYRFSIS WSRIFPNGTG MVNQEGVDYY NRLIDYMVKK 150
    GIKPYANLYH YDLPLALHEQ YLGWLSPNIV EAFADYADFC FQTFGDRVKD 200
    WFTFNEPRCV AALGYDNGFH APGRCSGCDA GGNSTTEPYL AAHHLILSHA 250
    AAVKRYREKY QLYQKGRIGI LLDFVWYEPF SDSNADRAAA QRARDFHLGW 300
    FLDPIIHGRY PYSMLEIVKD RMPTFSDEES RMVKDSIDYV GINHYTSFYM 350
    KDPGPWNLTP TSYQDDWHVG FAYERNGVPI GAQANSYWLY IVPWGINKAV 400
    TYVKETYGNP TMILSENGMD QPGNVSITQG VHDTVRIRYY RNYITELKKA 450
    IDDGAKVIGY FAWSLLDNFE WRLGYTSRFG IVYVDYKTLK RYPKDSAFWF 500
    KNMLSSKKRN 510
    Length:510
    Mass (Da):58,498
    Last modified:March 20, 2007 - v1
    Checksum:i9EBEAE24EE62833E
    GO

    Sequence cautioni

    The sequence BAF22416.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008213 Genomic DNA. Translation: BAF22416.1. Sequence problems.
    CM000144 Genomic DNA. Translation: EAZ40934.1.
    RefSeqiNP_001060502.1. NM_001067037.1.
    UniGeneiOs.20617.

    Genome annotation databases

    GeneIDi4344146.
    KEGGiosa:4344146.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008213 Genomic DNA. Translation: BAF22416.1 . Sequence problems.
    CM000144 Genomic DNA. Translation: EAZ40934.1 .
    RefSeqi NP_001060502.1. NM_001067037.1.
    UniGenei Os.20617.

    3D structure databases

    ProteinModelPortali A3BMZ5.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi A3BMZ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4344146.
    KEGGi osa:4344146.

    Organism-specific databases

    Gramenei A3BMZ5.

    Phylogenomic databases

    eggNOGi COG2723.

    Enzyme and pathway databases

    SABIO-RK A3BMZ5.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
      Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
      BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Rice family GH1 glycoside hydrolases with beta-D-glucosidase and beta-D-mannosidase activities."
      Kuntothom T., Luang S., Harvey A.J., Fincher G.B., Opassiri R., Hrmova M., Ketudat Cairns J.R.
      Arch. Biochem. Biophys. 491:85-95(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiBGL26_ORYSJ
    AccessioniPrimary (citable) accession number: A3BMZ5
    Secondary accession number(s): Q0D407
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3