A3A8Q4 (P2C18_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 18
Short name=OsPP2C18
EC=3.1.3.16

Gene names

Ordered Locus Names:	Os02g0599200, LOC_Os02g38710
ORF Names:	OsJ_007176, OSJNBa0038P01.37

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	804 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence BAD22514.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF09251.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 804	804	Probable protein phosphatase 2C 18		PRO_0000363264
Regions
	Transmembrane	19 – 39	21	Helical; Potential
	Domain	128 – 429	302	PP2C-like
Sites
	Metal binding	165	1	Manganese 1 By similarity
	Metal binding	165	1	Manganese 2 By similarity
	Metal binding	166	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	384	1	Manganese 2 By similarity
	Metal binding	425	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	407 – 408	2	VE → LK in CM000139. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	A3A8Q4 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 4965C18EC3B812DC	FASTA	804	84,982
	10 20 30 40 50 60 MGPLLERWIS REGRSDGGDA SGPVLFWCVL IIFAVPDAIR SSSLRLGQVA PRLVLFSNFK 70 80 90 100 110 120 AFRSPKFAKI LPGSDLFSPR VLGFRTRVVL LLDVFEVGFA LFCKASSTMG NSLPVESKVT 130 140 150 160 170 180 VEEENDRIKY IVSSMQGLGH KMEDAHAAIL SLDDTTSTSF FGVYDGHGGA EVASYCAKRF 190 200 210 220 230 240 HIELCNHEDY HNDLTNALDN VFFSMDENLQ QSDAWRELVI PRDNGWMYFL KAGVCANFWP 250 260 270 280 290 300 FPQAYTGPAY EGSTACVVVI RGDQMIVGHA GDSRCVLSRQ GGLAIDLSSD HKPRTSESER 310 320 330 340 350 360 ERVQNAGGIS LGVDCEKVME NYVIKEQWIL GYFGESVTIS RSIGDFAFKQ NKDLNREEQM 370 380 390 400 410 420 LICDPDIHTH DITGDMEFLV IASQGLWSCM ESADVVAYIH VRLLEGVELR VICEELVQSG 430 440 450 460 470 480 LASGENTTVI LVQFKPGAFQ FQYELVDPAA FDTAASNVAS TSAGPAGGSD SDTSATSDEG 490 500 510 520 530 540 VDDTATAGTT TTGYEAGSST GPGSGGGSAN AAFDSGGDLA ANLDIATNFG SDDLAANLDI 550 560 570 580 590 600 ATDIDTEDVF TFINSDDTFG INSDEVELDP NFRPKPQVRR AHDGPSPTPS EIEADLNASP 610 620 630 640 650 660 TRYNMRDIFE AFDKVEAELG GFPLQGHDVS STSTNPNTAT DTGSGSRTGD DDVDGAIARA 670 680 690 700 710 720 MAVASSVMTG AGYEVDSTTT NPSAAADTGS YTGDEIKVDD STSGSARGDS GELVNNDTTV 730 740 750 760 770 780 ADNNASGVAD STTVGDEVDP TATVAADDSN TGDKVDPPAI TKATADSNTS GEVDVDATAT 790 800 ATASASAAVA DDEGTAPDDS EGSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 343-804. Strain: cv. Nipponbare.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006457 Genomic DNA. Translation: BAD22514.1. Sequence problems. AP008208 Genomic DNA. Translation: BAF09251.1. Sequence problems. CM000139 Genomic DNA. No translation available. AK119492 mRNA. No translation available.
RefSeq	NP_001047337.1. NM_001053872.1.
UniGene	Os.49987.
3D structure databases
ProteinModelPortal	A3A8Q4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g38710.1; LOC_Os02g38710.1; LOC_Os02g38710.
GeneID	4329881.
KEGG	dosa:Os02t0599200-01. osa:4329881.
Organism-specific databases
Gramene	A3A8Q4.
Phylogenomic databases
eggNOG	COG0631.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 2 hits. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C18_ORYSJ

Accession

Primary (citable) accession number: A3A8Q4
Secondary accession number(s): Q6K1T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	April 3, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program