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Protein

Putative diaminopimelate epimerase, chloroplastic

Gene

DAPF

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative diaminopimelate epimerase, chloroplastic (DAPF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145By similarity1
Active sitei300By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative diaminopimelate epimerase, chloroplastic (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:DAPF
ORF Names:OsI_037517
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000007015 Componenti: Chromosome 12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47ChloroplastSequence analysisAdd BLAST47
ChainiPRO_000030718048 – 357Putative diaminopimelate epimerase, chloroplasticAdd BLAST310

Interactioni

Protein-protein interaction databases

STRINGi39946.BGIOSGA037614-PA.

Structurei

3D structure databases

ProteinModelPortaliA2ZLS4.
SMRiA2ZLS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi58 – 62Poly-Ala5

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHJB. Eukaryota.
COG0253. LUCA.
HOGENOMiHOG000220466.
OMAiMCGNGGR.
OrthoDBiEOG09360IPX.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase. 1 hit.
InterProiView protein in InterPro
IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiView protein in Pfam
PF01678. DAP_epimerase. 2 hits.
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiView protein in PROSITE
PS01326. DAP_EPIMERASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2ZLS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSATAAATA TIAAAAAAAA KLAATPAPAP SRRRLTLRGN PTARRCVAAM
60 70 80 90 100
AVSTPRSAAA AAFLERRESE RALHFVKYQG LGNDFIMMDN RDSAVPKVTP
110 120 130 140 150
EEAAKLCDRN FGVGADGVIF VMPGVNGADY TMRIFNSDGS EPEMCGNGVR
160 170 180 190 200
CFARFIAELE NLQGTHSFKI HTGAGLIIPE IQNDGKVKVD MGQPILSGPD
210 220 230 240 250
IPTKLPSTKN EAVVQADLAV DGSTWQVTCV SMGNPHCVTF GTKELKVLHV
260 270 280 290 300
DDLKLSDIGP KFEHHEMFPA RTNTEFVEVL SRSHLKMRVW ERGAGATLAC
310 320 330 340 350
GTGACAVVVA AVLEGRAERK CVVDLPGGPL EIEWREDDNH IYMTGPAEAV

FYGSAVH
Length:357
Mass (Da):38,075
Last modified:March 20, 2007 - v1
Checksum:i4454E4D184C04096
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000137 Genomic DNA. Translation: EAY83558.1.

Genome annotation databases

EnsemblPlantsiBGIOSGA037614-TA; BGIOSGA037614-PA; BGIOSGA037614.
GrameneiBGIOSGA037614-TA; BGIOSGA037614-PA; BGIOSGA037614.

Similar proteinsi

Entry informationi

Entry nameiDAPF_ORYSI
AccessioniPrimary (citable) accession number: A2ZLS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 20, 2007
Last modified: August 30, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families