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Protein

Chalcone synthase 1

Gene

CHS1

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671Acyl-thioester intermediatePROSITE-ProRule annotation
Binding sitei200 – 2001SubstrateBy similarity
Binding sitei311 – 3111Coenzyme A; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase 1 (EC:2.3.1.74)
Short name:
OsCHS1
Alternative name(s):
Naregenin-chalcone synthase
Gene namesi
Name:CHS1
Synonyms:CHS
ORF Names:OsI_035120
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000007015 Componenti: Chromosome 11

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Chalcone synthase 1PRO_0000293085Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39946.BGIOSGA033874-PA.

Structurei

Secondary structure

1
398
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 148Combined sources
Beta strandi21 – 288Combined sources
Beta strandi33 – 353Combined sources
Helixi36 – 383Combined sources
Helixi39 – 468Combined sources
Helixi53 – 6513Combined sources
Beta strandi70 – 723Combined sources
Helixi77 – 826Combined sources
Helixi84 – 863Combined sources
Beta strandi88 – 903Combined sources
Helixi94 – 12027Combined sources
Helixi124 – 1263Combined sources
Beta strandi129 – 1368Combined sources
Helixi143 – 1519Combined sources
Beta strandi158 – 1647Combined sources
Helixi169 – 18214Combined sources
Beta strandi188 – 1958Combined sources
Helixi197 – 1993Combined sources
Helixi209 – 2179Combined sources
Beta strandi221 – 23010Combined sources
Turni233 – 2353Combined sources
Beta strandi240 – 24910Combined sources
Beta strandi256 – 2627Combined sources
Beta strandi265 – 2706Combined sources
Helixi274 – 29017Combined sources
Helixi291 – 2933Combined sources
Helixi298 – 3003Combined sources
Beta strandi301 – 3055Combined sources
Helixi310 – 32011Combined sources
Helixi324 – 3274Combined sources
Helixi328 – 33710Combined sources
Helixi341 – 3433Combined sources
Helixi344 – 35815Combined sources
Turni364 – 3674Combined sources
Beta strandi369 – 3779Combined sources
Turni378 – 3803Combined sources
Beta strandi381 – 3899Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YJYX-ray1.86A/B1-398[»]
ProteinModelPortaliA2ZEX7.
SMRiA2ZEX7. Positions 6-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 658Coenzyme A bindingBy similarity
Regioni219 – 2202Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiENOG410IKG2. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
OMAiNICEYMA.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2ZEX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE
60 70 80 90 100
HMVELKEKFK RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI
110 120 130 140 150
VVVEVPKLGK AAAQKAIKEW GQPRSRITHL VFCTTSGVDM PGADYQLAKM
160 170 180 190 200
LGLRPNVSRL MMYQQGCFAG GTVLRVAKDL AENNRGARVL AVCSEITAVT
210 220 230 240 250
FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF QMVSASQTIL
260 270 280 290 300
PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS
310 320 330 340 350
IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD
360 370 380 390
EMRKRSAEDG HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA
Length:398
Mass (Da):43,238
Last modified:March 20, 2007 - v1
Checksum:i583FC50C65BB9755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89859 mRNA. Translation: CAA61955.1.
CM000136 Genomic DNA. Translation: EAY81161.1.
PIRiS58190.

Genome annotation databases

EnsemblPlantsiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
GrameneiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89859 mRNA. Translation: CAA61955.1.
CM000136 Genomic DNA. Translation: EAY81161.1.
PIRiS58190.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YJYX-ray1.86A/B1-398[»]
ProteinModelPortaliA2ZEX7.
SMRiA2ZEX7. Positions 6-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39946.BGIOSGA033874-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
GrameneiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.

Phylogenomic databases

eggNOGiENOG410IKG2. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
OMAiNICEYMA.

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Purple Puttu.
    Tissue: Leaf.
  2. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. 93-11.

Entry informationi

Entry nameiCHS1_ORYSI
AccessioniPrimary (citable) accession number: A2ZEX7
Secondary accession number(s): P48405, P93710, Q9AVC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 20, 2007
Last modified: May 11, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.