A2ZEX7 (CHS1_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase 1
Short name=OsCHS1
EC=2.3.1.74

Alternative name(s):
Naregenin-chalcone synthase

Gene names

Name:	CHS1
Synonyms:	CHS
ORF Names:	OsI_035120

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	auxin polar transport Inferred from electronic annotation. Source: EnsemblPlants/Gramene chalcone biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of anthocyanin biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to UV-B Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to gravity Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to jasmonic acid stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to oxidative stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to wounding Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleus Inferred from electronic annotation. Source: EnsemblPlants/Gramene plant-type vacuole membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Chalcone synthase 1		PRO_0000293085
Regions
	Region	58 – 65	8	Coenzyme A binding By similarity
	Region	219 – 220	2	Substrate binding By similarity
Sites
	Active site	167	1	Acyl-thioester intermediate By similarity
	Binding site	200	1	Substrate By similarity
	Binding site	311	1	Coenzyme A; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A2ZEX7 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 583FC50C65BB9755	FASTA	398	43,238
	10 20 30 40 50 60 MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE HMVELKEKFK 70 80 90 100 110 120 RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW 130 140 150 160 170 180 GQPRSRITHL VFCTTSGVDM PGADYQLAKM LGLRPNVSRL MMYQQGCFAG GTVLRVAKDL 190 200 210 220 230 240 AENNRGARVL AVCSEITAVT FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF 250 260 270 280 290 300 QMVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS 310 320 330 340 350 360 IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD EMRKRSAEDG 370 380 390 HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chalcone synthase cDNA from Oryza sativa." Scheffler B.E., Reddy A.R., Hoffmann I., Wienand U. Plant Gene Register PGR95-071 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Purple Puttu. Tissue: Leaf.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89859 mRNA. Translation: CAA61955.1. CM000136 Genomic DNA. Translation: EAY81161.1.
PIR	S58190.
UniGene	Os.12416.
3D structure databases
ProteinModelPortal	A2ZEX7.
SMR	A2ZEX7. Positions 6-392.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os11g32650.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os07t0501100-01.
Organism-specific databases
Gramene	A2ZEX7.
Phylogenomic databases
eggNOG	COG3424.
HOGENOM	HOG000245460.
Enzyme and pathway databases
UniPathway	UPA00154.
Gene expression databases
ArrayExpress	A2ZEX7.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS1_ORYSI

Accession

Primary (citable) accession number: A2ZEX7
Secondary accession number(s): P48405, P93710, Q9AVC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 20, 2007
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program