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Protein

Chalcone synthase 1

Gene

CHS1

Organism
Oryza sativa subsp. indica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167Acyl-thioester intermediatePROSITE-ProRule annotation1
Binding sitei200SubstrateBy similarity1
Binding sitei311Coenzyme A; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase 1 (EC:2.3.1.74)
Short name:
OsCHS1
Alternative name(s):
Naregenin-chalcone synthase
Gene namesi
Name:CHS1
Synonyms:CHS
ORF Names:OsI_035120
OrganismiOryza sativa subsp. indica (Rice)
Taxonomic identifieri39946 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000007015 Componenti: Chromosome 11

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002930851 – 398Chalcone synthase 1Add BLAST398

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39946.BGIOSGA033874-PA.

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 14Combined sources8
Beta strandi21 – 28Combined sources8
Beta strandi33 – 35Combined sources3
Helixi36 – 38Combined sources3
Helixi39 – 46Combined sources8
Helixi53 – 65Combined sources13
Beta strandi70 – 72Combined sources3
Helixi77 – 82Combined sources6
Helixi84 – 86Combined sources3
Beta strandi88 – 90Combined sources3
Helixi94 – 120Combined sources27
Helixi124 – 126Combined sources3
Beta strandi129 – 136Combined sources8
Helixi143 – 151Combined sources9
Beta strandi158 – 164Combined sources7
Helixi169 – 182Combined sources14
Beta strandi188 – 195Combined sources8
Helixi197 – 199Combined sources3
Helixi209 – 217Combined sources9
Beta strandi221 – 230Combined sources10
Turni233 – 235Combined sources3
Beta strandi240 – 249Combined sources10
Beta strandi256 – 262Combined sources7
Beta strandi265 – 270Combined sources6
Helixi274 – 290Combined sources17
Helixi291 – 293Combined sources3
Helixi298 – 300Combined sources3
Beta strandi301 – 305Combined sources5
Helixi310 – 320Combined sources11
Helixi324 – 327Combined sources4
Helixi328 – 337Combined sources10
Helixi341 – 343Combined sources3
Helixi344 – 358Combined sources15
Turni364 – 367Combined sources4
Beta strandi369 – 377Combined sources9
Turni378 – 380Combined sources3
Beta strandi381 – 389Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YJYX-ray1.86A/B1-398[»]
ProteinModelPortaliA2ZEX7.
SMRiA2ZEX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 65Coenzyme A bindingBy similarity8
Regioni219 – 220Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Phylogenomic databases

eggNOGiENOG410IKG2. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
OMAiNICEYMA.
OrthoDBiEOG09360E8Q.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A2ZEX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE
60 70 80 90 100
HMVELKEKFK RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI
110 120 130 140 150
VVVEVPKLGK AAAQKAIKEW GQPRSRITHL VFCTTSGVDM PGADYQLAKM
160 170 180 190 200
LGLRPNVSRL MMYQQGCFAG GTVLRVAKDL AENNRGARVL AVCSEITAVT
210 220 230 240 250
FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF QMVSASQTIL
260 270 280 290 300
PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS
310 320 330 340 350
IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD
360 370 380 390
EMRKRSAEDG HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA
Length:398
Mass (Da):43,238
Last modified:March 20, 2007 - v1
Checksum:i583FC50C65BB9755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89859 mRNA. Translation: CAA61955.1.
CM000136 Genomic DNA. Translation: EAY81161.1.
PIRiS58190.

Genome annotation databases

EnsemblPlantsiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
GrameneiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89859 mRNA. Translation: CAA61955.1.
CM000136 Genomic DNA. Translation: EAY81161.1.
PIRiS58190.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YJYX-ray1.86A/B1-398[»]
ProteinModelPortaliA2ZEX7.
SMRiA2ZEX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39946.BGIOSGA033874-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.
GrameneiBGIOSGA033874-TA; BGIOSGA033874-PA; BGIOSGA033874.

Phylogenomic databases

eggNOGiENOG410IKG2. Eukaryota.
COG3424. LUCA.
HOGENOMiHOG000245460.
OMAiNICEYMA.
OrthoDBiEOG09360E8Q.

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHS1_ORYSI
AccessioniPrimary (citable) accession number: A2ZEX7
Secondary accession number(s): P48405, P93710, Q9AVC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 20, 2007
Last modified: November 30, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.