A2ZC67 (ASP1_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartic proteinase Asp1
Short name=OSAP1
Short name=OsAsp1
EC=3.4.23.-

Alternative name(s):
Nucellin-like protein

Gene names

Name:	ASP1
ORF Names:	OsI_034160

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses protease activity in vitro. Ref.1
Tissue specificity	Expressed in pollen, nucellus, ovary wall, shoot and root meristem, coleoptiles of immature seeds, and somatic embryos. Ref.1
Developmental stage	Abundantly expressed in zygotic embryos 1 and 2 days after fertilization. Ref.1
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Aspartyl protease Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 46	23	Removed in mature form Potential		PRO_0000301660
	Chain	47 – 410	364	Aspartic proteinase Asp1		PRO_0000301661
Sites
	Active site	56	1	By similarity
	Active site	257	1	By similarity
Experimental info
	Sequence conflict	45	1	G → S in AAK81698. Ref.1
	Sequence conflict	45	1	G → S in AAK81699. Ref.1
	Sequence conflict	239	1	Q → H in AAK81698. Ref.1
	Sequence conflict	239	1	Q → H in AAK81699. Ref.1
	Sequence conflict	244 – 245	2	SK → KQS in AAK81699. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2ZC67 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: AAC9775F3234FB9A	FASTA	410	44,961
	10 20 30 40 50 60 MTARLALLAS LLLLLQLVPP SSAVVLELHG NVYPIGHFFV TMNIGDPAKP YFLDIDTGST 70 80 90 100 110 120 LTWLQCDYPC INCNKVPHGL YKPELKYAVK CTEQRCADLY ADLRKPMKCG PKNQCHYGIQ 130 140 150 160 170 180 YVGGSSIGVL IVDSFSLPAS NGTNPTSIAF GCGYNQGKNN HNVPTPVNGI LGLGRGKVTL 190 200 210 220 230 240 LSQLKSQGVI TKHVLGHCIS SKGKGFLFFG DAKVPTSGVT WSPMNREHKH YSPRQGTLQF 250 260 270 280 290 300 NSNSKPISAA PMEVIFDSGA TYTYFALQPY HATLSVVKST LSKECKFLTE VKEKDRALTV 310 320 330 340 350 360 CWKGKDKIRT IDEVKKCFRS LSLKFADGDK KATLEIPPEH YLIISQEGHV CLGILDGSKE 370 380 390 400 410 HPSLAGTNLI GGITMLDQMV IYDSERSLLG WVNYQCDRIP RSASAITSRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The rice nucellin gene ortholog OsAsp1 encodes an active aspartic protease without a plant-specific insert and is strongly expressed in early embryo." Bi X., Khush G.S., Bennett J. Plant Cell Physiol. 46:87-98(2005) [PubMed: 15659452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. IR64.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY043216 Genomic DNA. Translation: AAK81698.1. AY043217 mRNA. Translation: AAK81699.1. CM000136 Genomic DNA. No translation available.
UniGene	Os.50636.
3D structure databases
ProteinModelPortal	A2ZC67.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q6YNY7.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASP1_ORYSI

Accession

Primary (citable) accession number: A2ZC67
Secondary accession number(s): Q53NF8 , Q6YNY7, Q6YNY8, Q71SA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program