Reviewed, UniProtKB/Swiss-Prot A2Z1C8 (CESA9_ORYSI)

Names and origin

Protein names

Recommended name:
    Cellulose synthase A catalytic subunit 9 [UDP-forming]
    EC=2.4.1.12
Alternative name(s):
    OsCesA9

Gene names

Name:	CESA9
ORF Names:	OsI_030371

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1055 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity. Involved in the secondary cell wall formation.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Disruption phenotype	Plants develop a brittle culm (bc) phenotype with a reduction of up to 90% percent of cellulose content in culm. Ref.2
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1055	1055	Cellulose synthase A catalytic subunit 9 [UDP-forming]		PRO_0000319369
Regions
	Topological domain	1 – 268	268	Cytoplasmic Potential
	Transmembrane	269 – 289	21	Potential
	Topological domain	290 – 291	2	Extracellular Potential
	Transmembrane	292 – 312	21	Potential
	Topological domain	313 – 831	519	Cytoplasmic Potential
	Transmembrane	832 – 852	21	Potential
	Topological domain	853 – 860	8	Extracellular Potential
	Transmembrane	861 – 881	21	Potential
	Topological domain	882 – 899	18	Cytoplasmic Potential
	Transmembrane	900 – 920	21	Potential
	Topological domain	921 – 951	31	Extracellular Potential
	Transmembrane	952 – 972	21	Potential
	Topological domain	973 – 983	11	Cytoplasmic Potential
	Transmembrane	984 – 1004	21	Potential
	Topological domain	1005 – 1013	9	Extracellular Potential
	Transmembrane	1014 – 1034	21	Potential
	Topological domain	1035 – 1055	21	Cytoplasmic Potential
	Zinc finger	37 – 83	47	RING-type; degenerate
	Coiled coil	439 – 468	30	Potential
Sites
	Active site	387	1	Potential
	Active site	753	1	Potential
	Metal binding	37	1	Zinc 1 By similarity
	Metal binding	40	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	64	1	Zinc 1 By similarity
	Metal binding	67	1	Zinc 1 By similarity
	Metal binding	79	1	Zinc 2 By similarity
	Metal binding	82	1	Zinc 2 By similarity
	Binding site	553	1	Substrate Potential
	Binding site	555	1	Substrate Potential
Amino acid modifications
	Glycosylation	927	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A2Z1C8-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 8D050CC7A365D9EC	FASTA	1,055	118,697
	10 20 30 40 50 60 MEASAGLVAG SHNRNELVLI RGHEEPKPLR ALSGQVCEIC GDEVGRTVDG DLFVACNECG 70 80 90 100 110 120 FPVCRPCYEY ERREGTQNCP QCKTRYKRLK GSPRVPGDED EEDIDDLEHE FNIDDEKQKQ 130 140 150 160 170 180 LQQDQDGMQN SHITEAMLHG KMSYGRGPDD GDGNSTPLPP IITGARSVPV SGEFPISNSH 190 200 210 220 230 240 GHGEFSSSLH KRIHPYPVSE PGSAKWDEKK EVSWKERMDD WKSKQGIVAG GAPDPDDYDA 250 260 270 280 290 300 DVPLNDEARQ PLSRKVSIAS SKVNPYRMVI ILRLVVLGFF LRYRILHPVP DAIPLWLTSI 310 320 330 340 350 360 ICEIWFAVSW ILDQFPKWYP IDRETYLDRL SLRYEREGEP SLLSAVDLFV STVDPLKEPP 370 380 390 400 410 420 LVTANTVLSI LAVDYPVDKV SCYVSDDGAS MLTFESLSET AEFARKWVPF CKKFSIEPRA 430 440 450 460 470 480 PEFYFSQKVD YLKDKVHPNF VQERRAMKRE YEEFKVRINA LVAKAQKVPA EGWIMKDGTP 490 500 510 520 530 540 WPGNNTRDHP GMIQVFLGHS GGHDTEGNEL PRLVYVSREK RPGFQHHKKA GAMNALIRVS 550 560 570 580 590 600 AVLTNAPFML NLDCDHYINN SKAIREAMCF LMDPQVGRKV CYVQFPQRFD GIDVHDRYAN 610 620 630 640 650 660 RNTVFFDINM KGLDGIQGPV YVGTGCVFRR QALYGYNPPK GPKRPKMVTC DCCPCFGRKK 670 680 690 700 710 720 RKHGKDGLPE AVAADGGMDS DKEMLMSQMN FEKRFGQSAA FVTSTLMEEG GVPPSSSPAA 730 740 750 760 770 780 LLKEAIHVIS CGYEDKTDWG LELGWIYGSI TEDILTGFKM HCRGWRSVYC MPKRAAFKGS 790 800 810 820 830 840 APINLSDRLN QVLRWALGSV EIFFSRHSPL LYGYKNGNLK WLERFSYINT TIYPFTSLPL 850 860 870 880 890 900 LAYCTLPAVC LLTGKFIMPP ISTFASLFFI ALFISIFATG ILEMRWSGVS IEEWWRNEQF 910 920 930 940 950 960 WVIGGVSAHL FAVVQGLLKV LAGIDTNFTV TSKATGDEDD EFAELYAFKW TTLLIPPTTL 970 980 990 1000 1010 1020 LILNIIGVVA GVSDAINNGS EAWGPLFGKL FFAFWVIVHL YPFLKGLMGR QNRTPTIVVI 1030 1040 1050 WSVLLASIFS LLWVRIDPFT IKARGPDVRQ CGINC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[2]	"Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall." Tanaka K., Murata K., Yamazaki M., Onosato K., Miyao A., Hirochika H. Plant Physiol. 133:73-83(2003) [PubMed: 12970476] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000134 Genomic DNA. Translation: EAZ09139.1.
3D structure databases
SMR	A2Z1C8. Positions 22-95.
ModBase	Search...
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESA9_ORYSI

Accession

Primary (citable) accession number: A2Z1C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 20, 2007
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)