A2YZ01 (INV7_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 7
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 7
Invertase 7
OsCIN7
Sucrose hydrolase 7

Gene names

Name:	CIN7
Synonyms:	INV1
ORF Names:	OsI_029544

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in sucrose partitioning during seed development By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Sequence caution	The sequence AAD38399.1 differs from that shown. Reason: Frameshift at position 592. The sequence AAV28806.1 differs from that shown. Reason: Frameshift at position 592.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 596	572	Beta-fructofuranosidase, insoluble isoenzyme 7		PRO_0000303013
Regions
	Region	51 – 54	4	Substrate binding By similarity
	Region	115 – 116	2	Substrate binding By similarity
	Region	179 – 180	2	Substrate binding By similarity
Sites
	Active site	54	1	By similarity
	Binding site	70	1	Substrate By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	234	1	Substrate By similarity
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	552	1	N-linked (GlcNAc...) Potential
	Disulfide bond	432 ↔ 478		By similarity
Experimental info
	Sequence conflict	19	1	Missing in CM000134. Ref.2
	Sequence conflict	22	1	T → I in AAD38399. Ref.1
	Sequence conflict	309	1	L → V in AAD38399. Ref.1
	Sequence conflict	318	1	V → A in AAD38399. Ref.1
	Sequence conflict	381	1	W → R in AAD38399. Ref.1
	Sequence conflict	388	1	V → A in AAD38399. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2YZ01 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: EBEA9B1D5BAF188B	FASTA	596	65,561
	10 20 30 40 50 60 MARLGLAVCA ASFHLFLLLA STSSLRRAPT EADTANHARR TAYHFQPAKN WQNDPNGPMY 70 80 90 100 110 120 HNGMYHLFYQ YNPHSALWDI GNLSWGHSVS GDLLNWAALD TALDPTSPFD ANGCWSGSAT 130 140 150 160 170 180 ILPGALPAIL YTGIDASKEQ VQNVAFAKNP SDPLLREWEK PAYNPVIALP ADVPGDKFRD 190 200 210 220 230 240 PSTAWLGRDG LWRIAVSAEV DGVASTLVYR SKDFVRWERN AAPLHASRAA GMVECPDLFP 250 260 270 280 290 300 VAERGEDGLD TSANGAGGVR HVLKLSVMDT LQDYYMVGTY DDAADAFSPA EPERGDDCRS 310 320 330 340 350 360 WRRLDYGHLY ASKSFFDVRK NRRVLWAWAN ESDSQADDVA RGWSGVQTFP RKMWLAKDGK 370 380 390 400 410 420 QLLQWPIEEI ETLRRKRAGL WRGTRLGVGA VQEIVGVASS QADVEVVFKI PSLEEAERVD 430 440 450 460 470 480 DPNRLLDPQK LCGEKGAAVR GGVGPFGLLV MASGDLHEHT AVFFRVFRHH DKYKLLMCTD 490 500 510 520 530 540 LTKSSTRAGV YKPAYGGFVD MDIDDHKTIS LRTLIDHSVV ESFGGGGRAC ITARVYPEHV 550 560 570 580 590 ATSSSHLYVF NNGSDAVKVA KLEAWDLATA TVNVVVGDHH GLVAPALELE PTRTTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of rice invertase genes." Fernandes S.Q., Dennis E.S., Dolferus R. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. IR36.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[3]	"Evolution of rice invertase gene family." Ji X., Kathiresan A., Bennett J. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 503-596. Strain: cv. IR64.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155121 Genomic DNA. Translation: AAD38399.1. Frameshift. CM000134 Genomic DNA. No translation available. AY575555 mRNA. Translation: AAV28806.1. Frameshift.
3D structure databases
ProteinModelPortal	A2YZ01.
SMR	A2YZ01. Positions 36-575.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q56UC9.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV7_ORYSI

Accession

Primary (citable) accession number: A2YZ01
Secondary accession number(s): Q56UC9 , Q56UM5, Q6K311, Q9XGV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program