A2YVR7 (PSA2_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-2
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit B
20S proteasome subunit alpha-2

Gene names

Name:	PAB1
ORF Names:	OsI_028410

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.
Sequence caution	The sequence AAB82138.1 differs from that shown. Reason: Frameshift at position 229.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Proteasome subunit alpha type-2		PRO_0000301662
Experimental info
	Sequence conflict	23	1	E → N in AAB82138. Ref.1
	Sequence conflict	45 – 46	2	VI → FV in AAB82138. Ref.1
	Sequence conflict	72	1	I → V in AAB82138. Ref.1
	Sequence conflict	91	1	R → P in AAB82138. Ref.1
	Sequence conflict	97 – 98	2	YY → CC in AAB82138. Ref.1
	Sequence conflict	105	1	I → M in AAB82138. Ref.1
	Sequence conflict	178	1	Y → H in AAB82138. Ref.1
	Sequence conflict	191	1	A → G in AAB82138. Ref.1
	Sequence conflict	194	1	T → I in AAB82138. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A2YVR7 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: D98998C0BC6CD676	FASTA	235	25,844
	10 20 30 40 50 60 MGDSQYSFSL TTFSPSGKLV QIEHALTAVG SGQTSLGIKA ANGVVIATEK KLPSILVDET 70 80 90 100 110 120 SVQKIQSLTP NIGVVYSGMG PDFRVLVRKS RKQAQQYYRL YKETIPVTQL VRETAAVMQE 130 140 150 160 170 180 FTQSGGVRPF GVSLLIAGYD DNGPQLYQVD PSGSYFSWKA SAMGKNVSNA KTFLEKRYTE 190 200 210 220 230 DMELDDAIHT AILTLKEGYE GQISANNIEI GIIRSDREFK VLSPAEIKDF LEEVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of proteasome from rice." Lee M.C., Kim C.S., Eun M.Y. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Milyang 23. Tissue: Seed.
[2]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022735 mRNA. Translation: AAB82138.1. Frameshift. CM000133 Genomic DNA. No translation available.
PIR	T02089.
UniGene	Os.7900.
3D structure databases
ProteinModelPortal	A2YVR7.
SMR	A2YVR7. Positions 6-234.
ModBase	Search...
Protein-protein interaction databases
STRING	A2YVR7.
Proteomic databases
PRIDE	A2YVR7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g26970.1; LOC_Os03g26970.1; LOC_Os03g26970.
Organism-specific databases
Gramene	Q9LSU2.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA2_ORYSI

Accession

Primary (citable) accession number: A2YVR7
Secondary accession number(s): O22539, Q6AVF6, Q9LSU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program