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A2YB34 (TRMB_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:OsI_22311
OrganismOryza sativa subsp. indica (Rice)
Taxonomic identifier39946 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370581

Regions

Region103 – 1042S-adenosyl-L-methionine binding By similarity
Region136 – 1372S-adenosyl-L-methionine binding By similarity
Region234 – 2363S-adenosyl-L-methionine binding By similarity

Sites

Active site1591 By similarity
Binding site801S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1561S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A2YB34 [UniParc].

Last modified March 20, 2007. Version 1.
Checksum: 378AC5DAA0F9AAF9

FASTA25929,078
        10         20         30         40         50         60 
MASGDGANGG GGGGQGKLPR KRFYRARAHS NPLSDSHFPI PISPDEVDLS QHYPRYFPSG 

        70         80         90        100        110        120 
EGEARQGDAA VPRIRFADVG CGFGGLLVGL STLFPDTLMI GMELRDKVTE YVKERILALR 

       130        140        150        160        170        180 
ASNPGKYDNI SVVRTNSMKY IPNYFRKAQL SKMFFLFPDP HFKEKNHRRR VISMQLLDEY 

       190        200        210        220        230        240 
AYVMEVGGII YTITDVEELG EWMRSCLEKH PLFEAIPEEE TKADPVVKLL STATEEGQKV 

       250 
ARNGGQTFQA IFRRISLQE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000131 Genomic DNA. Translation: EAZ00295.1.

3D structure databases

ProteinModelPortalA2YB34.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os06g12990.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneA2YB34.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ORYSI
AccessionPrimary (citable) accession number: A2YB34
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 20, 2007
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways