Reviewed, UniProtKB/Swiss-Prot A2Y9C2 (LAC20_ORYSI)

Names and origin

Protein names

Recommended name:
    Laccase-20
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 20
    Urishiol oxidase 20
    Diphenol oxidase 20

Gene names

Name:	LAC20
ORF Names:	OsI_020915

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 580	557	Laccase-20		PRO_0000291906
Regions
	Domain	31 – 147	117	Plastocyanin-like 1
	Domain	156 – 310	155	Plastocyanin-like 2
	Domain	419 – 561	143	Plastocyanin-like 3
Sites
	Metal binding	81	1	Copper 1 By similarity
	Metal binding	83	1	Copper 2 By similarity
	Metal binding	126	1	Copper 2 By similarity
	Metal binding	128	1	Copper 3 By similarity
	Metal binding	478	1	Copper 4 Potential
	Metal binding	481	1	Copper 1 By similarity
	Metal binding	483	1	Copper 3 By similarity
	Metal binding	540	1	Copper 3 By similarity
	Metal binding	541	1	Copper 4 Potential
	Metal binding	542	1	Copper 2 By similarity
	Metal binding	546	1	Copper 4 Potential
	Metal binding	551	1	Copper 4 Potential
Amino acid modifications
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Glycosylation	429	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A2Y9C2-1 [UniParc]. Last modified March 20, 2007. Version 1. Checksum: 5482B2ED2D717F67	FASTA	580	64,907
	10 20 30 40 50 60 MVASLLCTVA VAVLAVAAVG GEAGVVEHTF VVHEMNVTHL CNTTKIFVVN GQLPGPTVDV 70 80 90 100 110 120 TEGDTVVVHV VNKIPHGLTI HWHGVRQLRS CWADGAGFIT ECPIPPGSER TYRFNVTDQV 130 140 150 160 170 180 GTLWWHAHVT CLRSTINGAF IIRPRDGKYP FPTPVKDVPI IIGEWWELDL VELDRRMRDG 190 200 210 220 230 240 NFDDNPLSAT INGKLGDLSN CSGIVEESFV LNVKHGESYL LRVINTAFFS EYYFKVAGHT 250 260 270 280 290 300 FTVVGADGNY LTPFKTDMVT VAPGEAIDVL MVADAPPAHY HMIVLANQPP EPDPQIPEYI 310 320 330 340 350 360 SRGLVRYTSA DANNNGLPVP MPIMPNQHNT MPSYYFHANL TGLMHPKHRR VPMHVDERIF 370 380 390 400 410 420 IILGLGTICR GRKHTCKRQR SLETIELSTM NNVSFTHPYT TALLERYYDG TPEGVYTEDF 430 440 450 460 470 480 PVRPPRPYNY TNPALIPPGP LEEVLEPTFK ATKLKRFKYN TSVEIIFQSS TLLMSDSNPM 490 500 510 520 530 540 HLHGYDVFLL AQGLGSFNAK RDIRKFNYHN PQLRNTILVP RGGWAAVRFI TDNPGMWYLH 550 560 570 580 CHFEFHIIMG MATAFIVEDG PTPETSLPPP PPEFKRCDAS

References

[1]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000131 Genomic DNA. Translation: EAY99682.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC20_ORYSI

Accession

Primary (citable) accession number: A2Y9C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	March 20, 2007
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)