A2Y053 (METK1_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 1
Short name=AdoMet synthase 1
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 1
Short name=MAT 1

Gene names

Name:	SAM1
Synonyms:	SAMS
ORF Names:	OsI_017696

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AdoMet synthase family.
Sequence caution	The sequence EAY96463.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthase 1		PRO_0000302887
Regions
	Nucleotide binding	122 – 127	6	ATP Potential
	Nucleotide binding	270 – 277	8	ATP Potential
Sites
	Metal binding	20	1	Magnesium By similarity
	Metal binding	46	1	Potassium By similarity
	Metal binding	274	1	Potassium By similarity
	Metal binding	282	1	Magnesium By similarity
	Binding site	150	1	ATP Potential
Experimental info
	Sequence conflict	157	1	E → K in CAC82203. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	A2Y053 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 16FE43474045FEB9	FASTA	396	43,220
	10 20 30 40 50 60 MAALDTFLFT SESVNEGHPD KLCDQVSDAV LDACLAEDPD SKVACETCTK TNMVMVFGEI 70 80 90 100 110 120 TTKANVDYEK IVRETCRNIG FVSADVGLDA DHCKVLVNIE QQSPDIAQGV HGHFTKRPEE 130 140 150 160 170 180 IGAGDQGHMF GYATDETPEL MPLSHVLATK LGARLTEVRK NGTCAWLRPD GKTQVTVEYR 190 200 210 220 230 240 NESGARVPVR VHTVLISTQH DETVTNDEIA ADLKEHVIKP VIPEQYLDEK TIFHLNPSGR 250 260 270 280 290 300 FVIGGPHGDA GLTGRKIIID TYGGWGAHGG GAFSGKDPTK VDRSGAYVAR QAAKSIVASG 310 320 330 340 350 360 LARRCIVQVS YAIGVPEPLS VFVDTYGTGR IPDKEILKIV KENFDFRPGM IIINLDLKKG 370 380 390 GNGRYLKTAA YGHFGRDDPD FTWEVVKPLK WEKPSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a S-adenosylmethionine synthetase gene in rice." van Breusegem F., Dekeyser R., Gielen J., van Montagu M., Caplan A. Plant Physiol. 105:1463-1464(1994) [PubMed: 7972513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Taichung native 1.
[2]	"Isolation and characterization of a new member of the rice S-adenosyl-L-methionine synthetase family." Mukhopadhyay A., Sharma S., Tyagi A.K. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Pusa Basmati. Tissue: Root.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26867 Genomic DNA. Translation: CAA81481.1. AJ296743 mRNA. Translation: CAC82203.1. CM000130 Genomic DNA. Translation: EAY96463.1. Sequence problems.
UniGene	Os.10130.
3D structure databases
ProteinModelPortal	A2Y053.
SMR	A2Y053. Positions 6-391.
ModBase	Search...
Protein-protein interaction databases
STRING	A2Y053.
Proteomic databases
PRIDE	A2Y053.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g04510.1; LOC_Os05g04510.1; LOC_Os05g04510. LOC_Os05g04510.2; LOC_Os05g04510.2; LOC_Os05g04510.
Organism-specific databases
Gramene	P46611.
Phylogenomic databases
PhylomeDB	A2Y053.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_ORYSI

Accession

Primary (citable) accession number: A2Y053
Secondary accession number(s): P46611, Q6AVZ2, Q8VXC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program