ID   GUN13_ORYSI             Reviewed;         625 AA.
AC   A2XYW8; Q259H9; Q7XQ92;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   22-FEB-2023, entry version 74.
DE   RecName: Full=Endoglucanase 13;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 13;
DE   AltName: Full=OsGLU6;
DE   Flags: Precursor;
GN   Name=GLU6; ORFNames=H0103C06.3, H0403D02.19, OsI_017261;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Guang-Lu-Ai No.4;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AL732344; CAH68191.1; -; Genomic_DNA.
DR   EMBL; AL732351; CAJ86099.1; -; Genomic_DNA.
DR   EMBL; CM000129; EAY96028.1; -; Genomic_DNA.
DR   AlphaFoldDB; A2XYW8; -.
DR   SMR; A2XYW8; -.
DR   STRING; 39946.A2XYW8; -.
DR   CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   GlyCosmos; A2XYW8; 1 site, No reported glycans.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   Proteomes; UP000007015; Chromosome 4.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR019028; CBM_49.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF41; ENDOGLUCANASE 5; 1.
DR   Pfam; PF09478; CBM49; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SMART; SM01063; CBM49; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cellulose degradation; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255"
FT   CHAIN           35..625
FT                   /note="Endoglucanase 13"
FT                   /id="PRO_0000295017"
FT   REGION          509..530
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        91
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        427
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        479
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        488
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CARBOHYD        440
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        230
FT                   /note="D -> H (in Ref. 2; EAY96028)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   625 AA;  68381 MW;  D220BF0D009441F1 CRC64;
     MAATMNKTPA TTFLLIPAAA SLVLLLAAAA SVEASAFDYA GAFDKCLLFF EAQRSGKLPD
     DRLVRWRGDS ALTDGFSQGV DLVGGYYDSG DHVKFGLPMA YAVTMLSWGV VEFEKEMVDG
     NKLHRVLDAI RWGTNYFVKA HTQHNALWVQ VGDGDSDHLC WERAEDMSTP RTAFKIDINN
     PGSEVAGETA AALAAAAKAF KPYDRMYSDL LLLHSKQLFT FADTFRGKYD DSLQSAKKFY
     PSASGYQDEL LWAAAWLYEA TGDEQYLRYV SQNAEAFGGT GWAVTEFSWD NKYAGLQVLL
     SKVLFEQGGS AAGYADTLKQ YQAKAEFFLC ACLQKNNGHN VKMTPGGLMY VSDWSNMQYV
     SSSAFLLTVY ADYLAESRGT LRCPDGEVKP AEILLFARSQ VDYVLGKNPK GMSYMVGYGS
     YYPTHVHHRG ASIPSIYAMN ATVGCMEGFD KYYNSKNADP NVLHGALVGG PDANDAYDDD
     RCNYQHAEPT LAGNAPMSGV FARLAASPAD NTPEYTPAPN APSPSNGGSP LEFVHTVTNT
     WKANGVDYYR HVVTAKNTCG HAITYLKLQI KELSGEIYGV SRTNAKDMYE FPSWMTRLDA
     GAQLTIVYIQ GGPAAKIAVV EYKTA
//