A2XX73 (ICMT_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein-S-isoprenylcysteine O-methyltransferase
EC=2.1.1.100

Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Prenylcysteine carboxyl methyltransferase

Gene names

Name:	ICMT
ORF Names:	B0403H10-OSIGBa0105A11.23, OsI_016666

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Cofactor	Divalent cations. Probably zinc By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	flower development Inferred from electronic annotation. Source: EnsemblPlants/Gramene shoot development Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Probable protein-S-isoprenylcysteine O-methyltransferase		PRO_0000356252
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A2XX73 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 049589ED58DF63EF	FASTA	191	22,071
	10 20 30 40 50 60 MAARAQAWLF AAALVIFHGS EYVLAAAFHG RRNVTATSLL ISKQYVLAMS FAMLEHLTEA 70 80 90 100 110 120 LLFPELKEYW FVSYVGLVMV IIGEVIRKLA VVTAGRSFTH VIRIHYEDQH KLITHGVYRL 130 140 150 160 170 180 MRHPGYSGFL IWAVGTQVML CNPLSTVAFT LVLWRFFSKR IPYEEFFLRQ FFGREYEEYA 190 QKVHSGLPFI E

References

[1]

"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Guang-Lu-Ai No.4.

[2]

"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR855228 Genomic DNA. Translation: CAH67871.1. CM000129 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	A2XX73.
Phylogenomic databases
eggNOG	COG2020.
HOGENOM	HOG000213961.
Gene expression databases
ArrayExpress	A2XX73.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. IPR025770. PPMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICMT_ORYSI

Accession

Primary (citable) accession number: A2XX73
Secondary accession number(s): Q01HS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	April 3, 2013
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program